A novel approach to control brown dog tick, Rhipicephalus sanguineus using sustained release poly-ɛ-caprolactone-pheromone microspheres
Control of brown dog tick, Rhipicephalus sanguineus was attempted by utilizing sustained release preparations of synthetic analogues of assembly pheromones. The assembly pheromone, in defined ratio, was encapsulated using poly-ɛ-caprolactone by water-in-oil-in-water double emulsion solvent evaporation technique. In the in vitro bioassay, percent mortality with test microspheres was 95.6, 64 and 44 among the unfed larvae, unfed nymph and unfed adults respectively, 24 hours post-exposure. Field trials were carried out to evaluate the efficacy of microspheres in luring and killing environmental stages of R. sanguineus in dog houses/kennels. Engorged and unfed stages in the environment were found adhered and dead on the specially designed lure.
KeywordsRhipicephalus sanguineus assembly pheromone sustained release poly-ɛ-caprolactone microspheres tick control
Unable to display preview. Download preview PDF.
- Benoit J.B., Lopez-Martinez G., Philips S.A., Elnitsky M.A., Yoder J.A., Lee R.E., Denlinger D.L. 2008. The seabird tick, Ixodes uriae, uses uric acid in penguin guano as a kairomone guanine in tick feces as an assembly pheromone on the Antarctic Peninsula. Polar Biology, 31, 1445–1451.CrossRefGoogle Scholar
- Leahy M.G. 1979. Pheromones of Argasid ticks. In: (Eds. J.G. Rodriguez) Recent Advances in Acarology, Academic Press, New York, 298–308.Google Scholar
- Lin W.J., Flanagan D.R., Linhardt R.J. 1999. A novel fabrication of poly-ɛ-caprolactone particles for cotrolled insulin delivery.Google Scholar
- Mukerjee A., Sinha V.R., Pruthi V. 2007. Preparation and characterization of poly-ɛ-caprolactone particles for controlled insulin delivery. Journal of Biomedical and Pharmaceutical Engineering, 1, 40–44.Google Scholar
- Ranju R.S. 2011. Application of semiochemicals for the control of Ixodid ticks. M.V.Sc., thesis. Submitted to Tamil Nadu Veterinary and Animal Sciences University, Chennai.Google Scholar