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Acta Parasitologica

, Volume 59, Issue 1, pp 1–4 | Cite as

PCR detection of Neospora caninum in water buffalo foetal tissues

  • Clementina Auriemma
  • Maria Gabriella Lucibelli
  • Giorgia Borriello
  • Esterina De Carlo
  • Alessandra Martucciello
  • Lorena Schiavo
  • Amalia Gallo
  • Francesca Bove
  • Federica Corrado
  • Santa Girardi
  • Maria Grazia Amoroso
  • Barbara Ďegli Uberti
  • Giorgio Galiero
Original Paper

Abstract

The seroprevalence of Neospora caninum was surveyed by an ELISA kit on two water buffalo herds of Southern Italy. Seropositive samples were detected in 47% and 59% of individuals, respectively, thus indicating high level of exposure to the parasite even if the possibility of vertical transmission cannot be excluded. Tissue samples collected from three aborted fetuses from the same herds were investigated for N. caninum presence by PCR assays targeting the 18S and the Nc5 DNA sequences, respectively. Both methods have shown the presence of N. caninum DNA in heart and brain. Sequencing of the Nc5 genomic DNA confirmed the presence of N. caninum in the samples; phylogenetic analysis of the obtained sequences showed high homology among the Neospora recovered from different samples. The present study suggests an important role of N. caninum as a possible abortive agent for water buffaloes.

Keywords

Neospora caninum water buffalo abortion PCR 

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Copyright information

© Versita Warsaw and Springer-Verlag Wien 2014

Authors and Affiliations

  • Clementina Auriemma
    • 1
  • Maria Gabriella Lucibelli
    • 1
  • Giorgia Borriello
    • 1
  • Esterina De Carlo
    • 2
  • Alessandra Martucciello
    • 2
  • Lorena Schiavo
    • 2
  • Amalia Gallo
    • 1
  • Francesca Bove
    • 1
  • Federica Corrado
    • 1
  • Santa Girardi
    • 1
  • Maria Grazia Amoroso
    • 1
  • Barbara Ďegli Uberti
    • 1
  • Giorgio Galiero
    • 1
  1. 1.Department of Animal HealthIstituto Zooprofilattico Sperimentale del MezzogiornoPortici, NAItaly
  2. 2.Centro di Referenza Nazionale sull’Igiene e le Tecnologie dell’Allevamento e delle Produzioni BufalineFuorni, SAItaly

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