Kinetics of anti-Neospora antibodies during the period of two consecutive pregnancies in chronically infected dairy cows
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The course of anti-Neospora antibody kinetics during two consecutive pregnancies has been evaluated in five chronically infected dairy cows. The blood samples of cows were collected monthly. Anti-Neospora antibodies in blood sera were detected by indirect ELISA (ID-VET, France). During whole period of the study cows remained seropositive; with S/P% values (iELISA) ranging from 94 to 214%. The antibody kinetics determined by iELISA showed a significant increase (P<0.0059) of specific IgG antibodies in the third trimester of both pregnancies. The monitored cows gave birth to 10 healthy calves (4 steers and 6 heifers). To confirm the occurrence of vertical transmission of Neospora from mothers to offspring in the herd, dam-daughter serology was performed. Anti-Neospora antibodies was found in 4 from 6 heifers (>6 months old). Study presents original data reporting on the very similar persistent pattern of anti-Neospora antibody levels during the third trimester of pregnancies in all five dairies. Based on the high seropositivity of female offspring, the reactivation of a latent infection of cows rather than a re-infection can be supposed.
KeywordsNeospora caninum cows pregnancy indirect ELISA
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- Andrianarivo A.G., Anderson M.L., Rowe J.D., Gardne I.A., Reynolds J.P., Choromanski L., Conrad P.A. 2005. Immune responses during pregnancy in heifers naturally infected with Neospora caninum with and without immunization. Parasitology Research, 96, 24–31. DOI: 10.1007/s00436-005-1313-y.PubMedCrossRefGoogle Scholar
- Björkman C, Johansson O, Stenlund S, Holmdahl OJM, Uggla A. 1996. Neospora species infection in a herd of dairy cattle. Journal of American Veterinary Medicine Association, 208, 1441–1444.Google Scholar
- Fioretti D. P., Pasquai P., Diaferia M., Mangili V., Rosignoli L. 2003. Neospora caninum infection and congenital transmission: serological and parasitological study of cows up to the fourth gestation. Journal of Veterinary Medicine B, 50, 399–404. DOI: 10.1046/j.1439-0450.2003.00686.x.CrossRefGoogle Scholar
- Guy C.S., Williams D.J.L., Kelly D.F., McGarry J.W., Guy F., Björkman C., Smith R.F., Trees A.J. 2001. Neospora caninum in persistently infected pregnant cows: spontaneous transplacental infection is associated with an acute increase in maternal antibody. Veterinary Record, 149, 443–449.PubMedCrossRefGoogle Scholar
- Pereira-Bueno J., Quintanilla-Gozalo A., Seijas-Carbalfedo A., Costas E., Ortega-Mora L.M. 2000. Observational studies in Neospora caninum infected dairy cattle: pattern of transmission and age-related antibody fluctuations. In: (Eds. A. Hemphill; B. Gottstein) A European perspective on Neospora caninum. International Journal for Parasitology, 30, 906–909.Google Scholar
- Rinaldi L., Fusco G., Musella V., Veneziano V., Guarino A., Taddei R., Cringoli G. 2005. Neospora caninum in pastured cattle: determination of climatic, environmental, farm management and individual animal risk factors using remote sensing and geographical information systems. Veterinary Parasitology, 128, 219–30. DOI: 10.1016/j.vetpar.2004.12.011.PubMedCrossRefGoogle Scholar
- Stenlund S., Kindahl H., Magnusson U., Uggla A., Bjorkman C. 1999. Serum antibody profi1e and reproductive performance during two consecutive pregnancies of cows naturally infected with Neospora caninum. Veterinary Parasitology, 85, 227–234. DOI: 10.1016/S0304-4017(99)00120-X.PubMedCrossRefGoogle Scholar
- Youssefi M.R., Ebrahimpour S., Esfandiari B. 2010. Survey of Neospora caninum antibody in aborting cattle from three climate regions of Iran. World Applied Sciences Journal, 10, 1448–1451.Google Scholar