Acta Parasitologica

, Volume 58, Issue 4, pp 541–546 | Cite as

Toxoplasma gondii, Neospora caninum and tick-transmitted bacterium Anaplasma phagocytophilum infections in one selected goat farm in Slovakia

  • Andrea Čobádiová
  • Katarina Reiterová
  • Markéta Derdáková
  • Silvia Špilovská
  • Ľudmila Turčeková
  • Ivana Hviščová
  • Vladimir Hisira
Original Paper
  • 126 Downloads

Abstract

Parasitic diseases of livestock together with poor welfare conditions can negatively affect the health status and production of small ruminants. Protozoan parasites and tick-borne infectious agents are common threat of livestock including small ruminants mostly during the pasture season. Therefore the priority of the study was to analyse the circulation and presence of two protozoan parasites Toxoplasma gondii and Neospora caninum as well as tick-transmitted bacterium Anaplasma phagocytophilum in one selected goat farm in Eastern Slovakia. Throughout a three-year study period we have repeatedly screened the sera and blood of goats and dogs from monitored farm. In total, 343 blood serum samples from 116 goats were examined by ELISA. The mean seropositivity for T. gondii was 56.9% (66/116, CI (95%) = 48–66.0) and 15.5% (18/116, CI (95%) = 9.3–22.7) for N. caninum. The permanent occurrence of anti-Toxoplasma and anti-Neospora antibodies was detected in repeatedly examined goats during the whole monitored period. The presence of both parasites in the flock was analysed by PCR. DNA of T. gondii was confirmed in 12 out of 25 Toxoplasma-seropositive goats and N. caninum in 14 samples out of 18 Neospora-seropositive animals; four goats were co-infected with both pathogens. The risk of endogenous transmission of both parasites was pursued by examination of 41 kid’s sera, where seropositivity for toxoplasmosis was 31.7% and for neosporosis 14.6%. In dogs 61.1% seropositivity for T. gondii and 38.9% for N. caninum was found, however, their faeces were negative for coccidian oocysts. Eight out of 108 tested animals were infected with A. phagocytophilum, the causative agent of tick-borne fever. Seven of them were simultaneously infected with T. gondii and A. phagocytophilum, out of which four goats were concurrently infected with all three pathogens.

Keywords

Toxoplasma gondii Neospora caninum Anaplasma phagocytophilum ELISA PCR goats dogs 

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Copyright information

© Versita Warsaw and Springer-Verlag Wien 2013

Authors and Affiliations

  • Andrea Čobádiová
    • 1
  • Katarina Reiterová
    • 1
  • Markéta Derdáková
    • 1
    • 3
  • Silvia Špilovská
    • 1
  • Ľudmila Turčeková
    • 1
  • Ivana Hviščová
    • 1
  • Vladimir Hisira
    • 2
  1. 1.Institute of Parasitology of the Slovak Academy of SciencesKošiceSlovak Republic
  2. 2.University of Veterinary Medicine and Pharmacy in KošiceKošiceSlovak Republic
  3. 3.Institute of Zoology of the Slovak Academy of SciencesBratislava — Karlova VesSlovak Republic

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