Toxoplasma gondii, Neospora caninum and tick-transmitted bacterium Anaplasma phagocytophilum infections in one selected goat farm in Slovakia
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Parasitic diseases of livestock together with poor welfare conditions can negatively affect the health status and production of small ruminants. Protozoan parasites and tick-borne infectious agents are common threat of livestock including small ruminants mostly during the pasture season. Therefore the priority of the study was to analyse the circulation and presence of two protozoan parasites Toxoplasma gondii and Neospora caninum as well as tick-transmitted bacterium Anaplasma phagocytophilum in one selected goat farm in Eastern Slovakia. Throughout a three-year study period we have repeatedly screened the sera and blood of goats and dogs from monitored farm. In total, 343 blood serum samples from 116 goats were examined by ELISA. The mean seropositivity for T. gondii was 56.9% (66/116, CI (95%) = 48–66.0) and 15.5% (18/116, CI (95%) = 9.3–22.7) for N. caninum. The permanent occurrence of anti-Toxoplasma and anti-Neospora antibodies was detected in repeatedly examined goats during the whole monitored period. The presence of both parasites in the flock was analysed by PCR. DNA of T. gondii was confirmed in 12 out of 25 Toxoplasma-seropositive goats and N. caninum in 14 samples out of 18 Neospora-seropositive animals; four goats were co-infected with both pathogens. The risk of endogenous transmission of both parasites was pursued by examination of 41 kid’s sera, where seropositivity for toxoplasmosis was 31.7% and for neosporosis 14.6%. In dogs 61.1% seropositivity for T. gondii and 38.9% for N. caninum was found, however, their faeces were negative for coccidian oocysts. Eight out of 108 tested animals were infected with A. phagocytophilum, the causative agent of tick-borne fever. Seven of them were simultaneously infected with T. gondii and A. phagocytophilum, out of which four goats were concurrently infected with all three pathogens.
KeywordsToxoplasma gondii Neospora caninum Anaplasma phagocytophilum ELISA PCR goats dogs
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- Bártová E., Sedlák K. 2012. Toxoplasma gondii and Neospora caninum antibodies in goats in the Czech Republic. Veterinární Medicína. 57, 111–114.Google Scholar
- Derdáková M., Štefančíková A., Špitálska E., Tarageľová V., Košťálová T., Hrkľová G., Kybicová K., Schánilec P., Majláthová V., Várady M., Peťko B. 2011. Emergence and genetic variability of Anaplasma species in small ruminants and ticks from Central Europe. Veterinary Microbiology, 153, 293–298. DOI: 10.1016/j.vetmic.2011.05.044PubMedCrossRefGoogle Scholar
- Dubey J.P., Rajendran C., Ferreira L.R., Martins J., Kwok O.C.H., Hill D.E., Villena I., Zhou, H., Su C., Jones J.L. 2011a. High prevalence and genotypes of Toxoplasma gondii isolated from goats, from a retail meat store, destined for human consumption in the USA. International Journal for Parasitology, 4, 827–833. DOI: 10.1016/j.ijpara.2011.03.006.CrossRefGoogle Scholar
- Dumler J.S., Barbet A.F., Bekker C.P., Dasch G.A., Palmer G.H., Ray S.C., Rikihisa Y., Rurangirwa F.R. 2001. Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and ‘HGE agent’ as subjective synonyms of Ehrlichia phagocytophila. International Journal of Systematic and Evolutionary Microbiology, 51, 2145–2165.PubMedCrossRefGoogle Scholar
- De la Fuente J., Massung R.F., Wong S.J., Chu F.K., Lutz H., Meli M., Loewenich F.D., Grzeszczuk A., Torina A., Caracappa S., Mangold A.J., Naranjo V., Stuen S., Kocan K.M. 2005. Sequence analysis of the msp4 gene of Anaplasma phagocytophilum strains. Journal of Clinical Microbiology, 43, 1309–1317. DOI: 10.1128/JCM.43.3.1309-1317.2005.PubMedCrossRefPubMedCentralGoogle Scholar
- Lamoril J., Molina J.M., De Gouvello A., Garin Y.J., Deybach J.C., Modai J., Derouin F. 1996. Detection by PCR of Toxoplasma gondii in blood in the diagnosis of cerebral toxoplasmosis in patients with AIDS. Journal of Clinical Pathology, 49, 89–92. DOI: 10.1136/jcp.49.1.89.PubMedCrossRefPubMedCentralGoogle Scholar
- Neto J.O.A., Azevedo S.S., Gennari S.M., Funada M.R., Pena H.F.J., Araújo A.R.C.P., Batista C.S.A., Silva M.L.C.R., Gomes A.A.B., Piatti R.M., Alves C.J. 2008. Prevalence and risk factors for anti-Toxoplasma gondii antibodies in goats of the Seridó Oriental microregion, Rio Grande do Norte state, Northeast region of Brazil. Veterinary Parasitology, 156, 329–332. DOI: 10.1016/j.vetpar.2008.05.013.PubMedCrossRefGoogle Scholar
- Scott G. R., 1991. Tick-borne infections. In: (Eds. W.B. Martin, I.D. Aitken) Diseases of Sheep. 2nd edition Blackwell Scientific Publications, Oxford, 327–336.Google Scholar
- Spišák F., Turčeková L., Reiterová K., Špilovská S., Dubinský P. 2010. Prevalence estimation and genotypization of Toxoplasma gondii in goats. Biologica. 65, 670–674. DOI:10.2478/s11756-010-0070-2.Google Scholar
- Štefanidesová K., Kocianová E., Boldiš V., Košťanová Z., Kanka P., Nemethová D., Špitalská E. 2008. Evidence of Anaplasma phagocytophilum and Rickettsia helvetica infection in free-ranging ungulates in central Slovakia. European Journal of Wildlife Research, 54, 519–524. DOI: 10.1007/s10344-007-0161-8.CrossRefGoogle Scholar