Acta Parasitologica

, Volume 58, Issue 4, pp 504–514 | Cite as

Gonad-infecting philometrid Philometra philippinensis sp. nov. (Nematoda, Philometridae) from the bigeye barracuda Sphyraena forsteri Cuvier (Sphyraenidae) off Mariveles, Bataan Province, Philippine archipelago

  • Karl Marx A. Quiazon
  • Tomoyoshi Yoshinaga
Original Paper


We conducted a parasitological study to identify nematodes infecting the gonads of bigeye barracuda Sphyraena forsteri Cuvier (Sphyraenidae) caught in the West Philippine Sea, off the Mariveles coast, Bataan Province, the Philippine archipelago. Males and very tiny nongravid female philometrid nematodes were collected from the ovaries of the fish. Based on light and scanning electron microscope observations, and supported by molecular analyses of the partial nucleotide sequences of the 18S small subunit ribosomal DNA, the philometrid nematode was determined to be a new species belonging to the genus Philometra Costa, 1845 in the family Philometridae (Nematoda: Dracunculoidea), and was named P. philippinensis sp. nov. This new species was clearly distinguishable morphologically from other congeners that parasitize the gonads of marine fishes. Males have unique sickle-shaped spicules and gubernaculum, and a vulva-like cloacal opening. Following Dentirumai philippinensis Quiazon et Moravec, 2013 and Philometra robusta Moravec, Möller et Heeger, 1992, this is the third philometrid species and the first gonad-infecting philometrid from the Philippine archipelago.


Philometridae 18S small subunit rDNA gonad-infecting philometrid nematodes Philometra philippinensis Sphyraena forsteri Philippine archipelago 


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  1. Arthur R.J., Lumanlan-Mayo S. 1997. Checklist of the parasites of fishes of the Philippines. FAO Fisheries Tech Paper 369. FAO, Rome.Google Scholar
  2. Baruš V., Jarkovsky J., Prokeš M. 2007. Philometra ovata (Nematoda: Philometridae): a potential sentinel species of heavy metal accumulation. Parasitology Research, 100, 929–933.PubMedCrossRefGoogle Scholar
  3. Carpenter K.E., Springer V.G. 2005. The center of the center of marine shore fish biodiversity: the Philippine Islands. Environmental Biology of Fishes, 72, 467–480.CrossRefGoogle Scholar
  4. Clarke L.M., Dove A.D.M., Conover D.O. 2006. Prevalence, intensity, and effect of a nematode (Philometra saltatrix) in the ovaries of bluefish (Pomatomus saltatrix). Fisheries Bulletin, 104, 118–124.Google Scholar
  5. Deepthi G.R., Sreekala L.K., Sunil M.S., Radhakrishnan T., Radhakrishnan S. 2007. Molecular stress response of the longarm mullet, Valamugil cunnesius (Val.), to Philometra cephalus (Nematoda) infection. Indian Journal of Multidisciplinary Research, 3, 327–334.Google Scholar
  6. Froese R., Pauly D. (Eds). 2013. FishBase. World Wide Web electronic publication,, version 2/2013.Google Scholar
  7. Gaglio G., Giannetto S., Panebianco A., Moravec F. 2009. First description of the male of Philometra filiformis (Nematoda: Philometridae), a gonad-infecting parasite of the marine fish Pagellus erythrinus (Sparidae) in Mediterranean. Folia Parasitologica, 56, 317–318.PubMedGoogle Scholar
  8. González-Solís D., Moravec F., Tuz Paredes V.M. 2007. A new species of Dentiphilometra (Nematoda: Philometridae) from the musculature of the gray snapper Lutjanus griseus (Osteichthyes) off the Caribbean coast of Mexico. Journal of Parasitology, 93, 1132–1135.PubMedCrossRefGoogle Scholar
  9. Hall T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.Google Scholar
  10. Hesp S.A., Hobbs R.P., Potter I.C. 2002. Infection of the gonads of Glaucosoma hebraicum by the nematode Philometra lateolabracis: occurrence and host response. Journal of Fish Biology, 60, 663–673.Google Scholar
  11. Hutson K.S., Brock E.L., Steer M.A. 2011. Spatial variation in parasite abundance: evidence of geographical population structuring in southern garfish Hyporhamphus melanochir. Journal of Fish Biology, 78, 166–182.PubMedCrossRefGoogle Scholar
  12. Kåll S., Fagerholm H.P., Sarvala J. 2004. Pathogenicity of the gill artery worm Philometra obturans (Nematoda) in northern pike (Esox lucius) in Southwest Finland. Journal of Parasitology, 90, 177–181.PubMedCrossRefGoogle Scholar
  13. Merella P., Reñones O., Garippa G. 2005. Reinstatement of Philometra jordanoi (López-Neyra, 1951) (Nematoda: Philometridae): a parasite of the Mediterranean dusky grouper Epinephelus marginatus (Lowe) (Osteichthyes: Serranidae). Systematic Parasitology, 61, 203–206.PubMedCrossRefGoogle Scholar
  14. Moravec F. 2004. Some aspects of the taxonomy and biology of dracunculoid nematodes parasitic in fishes: a review. Folia Parasitologica, 51, 1–13.PubMedGoogle Scholar
  15. Moravec F. 2006. Dracunculoid and anguillicoloid nematodes parasitic in vertebrates. Academia, Prague, 634 pp.Google Scholar
  16. Moravec F., Bakenhaster M. 2010. A new species of Philometra (Nematoda: Philometridae) from the sand perch Diplectrum formosum (Serranidae) off Florida, northern Gulf of Mexico. Journal of Parasitology, 96, 987–992.PubMedCrossRefGoogle Scholar
  17. Moravec F., Bakenhaster M., Fajer-Avila E.J. 2010a. New philometrids (Nematoda, Philometridae) from head tissues of two serranid fishes (Epinephelus morio and Mycteroperca microlepis) off Florida, northern Gulf of Mexico. Acta Parasitologica, 55, 359–368.CrossRefGoogle Scholar
  18. Moravec F., Chávez R.A., Oliva M.E. 2011b. A new gonad-infecting species of Philometra (Nematoda: Philometridae) from the red cusk-eel Genypterus chilensis (Osteichthyes: Ophidiidae) off Chile. Parasitology Research, 108, 227–232.PubMedCrossRefGoogle Scholar
  19. Moravec F., Crosby M.D., de Buron I., González-Solís D., Roumillat W.A. 2008c. Three new species of philometrids (Nematoda: Philometridae) from centrarchid fishes in the USA. Journal of Parasitology, 94, 1103–1113.PubMedCrossRefGoogle Scholar
  20. Moravec F., de Buron I. 2013. A synthesis of our current knowledge of philometrid nematodes, a group of increasingly important fish parasites. Folia Parasitologica, 60, 81–101.PubMedGoogle Scholar
  21. Moravec F., de Buron I., Baker T.G., González-Solís D. 2008a. Some gonad-infecting species of Philometra (Nematoda, Philometridae) from offshore fishes of South Carolina and Georgia, USA, including Philometra charlestonensis sp. nov. from the scamp Mycteroperca phenax. Acta Parasitologica, 53, 382–391.CrossRefGoogle Scholar
  22. Moravec F., de Buron I. 2009. New data on three gonad-infecting species of Philometra (Nematoda, Philometridae) from estuarine fishes in South Carolina, USA. Acta Parasitologica, 54, 244–252.CrossRefGoogle Scholar
  23. Moravec F., Fajer-Avila E.J., Bakenhaster M. 2010b. Philometra floridensis sp. n. (Nematoda: Philometridae) from the ovary of red drum Sciaenops ocellatus (Osteichthyes: Sciaenidae) off the coast of Florida, USA. Journal of Helminthology, 84, 49–54.PubMedCrossRefGoogle Scholar
  24. Moravec F., Gopalakrishnan A., Rajkumar M., Saravanakumar A., Kaliyamoorthy S. 2011a. A new gonad-infecting species of Philometra Costa, 1845 (Nematoda: Philometridae) from the marine fish Terapon jarbua (Forsskål) (Terapontidae) off the eastern coast of India. Systematic Parasitology, 80, 23–33.PubMedCrossRefGoogle Scholar
  25. Moravec F., Justine J.-L. 2008. Some philometrid nematodes (Philometridae), including four new species of Philometra, from marine fishes off New Calendonia. Acta Parasitologica, 53, 369–381.CrossRefGoogle Scholar
  26. Moravec F., Justine J.-L. 2009. New data on dracunculoid nematodes from fishes off New Caledonia, including four new species of Philometra (Philometridae) and Ichthyofilaria (Guyanemidae). Folia Parasitologica, 56, 129–142.PubMedGoogle Scholar
  27. Moravec F., Justine J.-L. 2011. Two new gonad-infecting Philometra species (Nematoda: Philometridae) from the marine fish Lutjanus vita (Perciformes: Lutjanidae) off New Caledonia. Folia Parasitologica, 58, 302–310.PubMedGoogle Scholar
  28. Moravec F., Magi M., Macchioni F. 2008b. Redescription of the gonad-infecting nematode Philometra saltatrix Ramachandran, 1973 (Philometridae) based on specimens from the type host Pomatomus saltatrix (L.) (Osteichthyes) from the Tuscan Sea, Italy. Folia Parasitologica, 55, 219–223.PubMedGoogle Scholar
  29. Moravec F., Manoharan J. 2013. Gonad-infecting philometrids (Nematoda: Philometridae) including four new species from marine fishes off the eastern coast of India. Folia Parasitologica, 60, 105–122.PubMedGoogle Scholar
  30. Moravec F., Möller H., Heeger T. 1992. Philometra robusta sp. n. (Nematoda: Philometridae) from the abdominal cavity of the scribbled toadfish, Arothron mappa (Lesson) from the Philippines. Folia Parasitologica, 39, 227–234.Google Scholar
  31. Moravec F., Salgado-Maldonado G. 2007. A new species of Philometra (Nematoda, Philometridae) from the gonads of the rock hind Epinephelus adscensionis (Osteichthyes) from the southern Gulf of Mexico. Acta Parasitologica, 52, 376–381.CrossRefGoogle Scholar
  32. Moravec F., Walter T., Yuniar A.T. 2012. Five new species of philometrid nematodes (Philometridae) from marine fishes off Java, Indonesia. Folia Parasitologica, 59, 115–130.PubMedGoogle Scholar
  33. Parukhin A.M. 1971. Nematodes from fishes of the Red Sea and Indian Ocean. Vol. “Voprosy ekologii ryb yuzhnykh morey”, Biologiya Morya 23. Naukova Dumka, Kiev, pp. 177–193. (In Russian).Google Scholar
  34. Perez G.R., Roumillat W.A., Levesque E.M., Connors V.A., De Buron I. 2009. Synchronization of occurrence of the ovarian philometrid, Philometra cardinensis, with the spawning season of its host, the spotted sea trout, Cynoscion nebulosus. Parasitology Research, 104, 1079–1085.PubMedCrossRefGoogle Scholar
  35. Petersen F., Palm H., Möller H., Cuzi M.A. 1993. Flesh parasites of fish from central Philippine waters. Diseases of Aquatic Organisms, 15, 81–86.CrossRefGoogle Scholar
  36. Portes Santos C., Moravec F. 2009. Tissue-dwelling philometrid nematodes of the fish Arapaima gigas in Brazil. Journal of Helminthology, 83, 295–301.PubMedCrossRefGoogle Scholar
  37. Quiazon K.M.A., Moravec F. 2013. Dentirumai philippinensis n. gen., n. sp. (Nematoda: Philometridae), a new tissue-infecting philometrid nematode from the loach goby Rhyacichthys aspro (Valenciennes) (Rhyacichthyidae) in the Philippines. Journal of Helminthology, 87, 59–65.PubMedCrossRefGoogle Scholar
  38. Quiazon K.M.A., Yoshinaga T., Ogawa K. 2008a. Taxonomical study into two new species of Philometra (Nematoda: Philometridae) previously identified as Philometra lateolabracis (Yamaguti, 1935). Folia Parasitologica, 55, 29–41.PubMedGoogle Scholar
  39. Quiazon K.M.A., Yoshinaga T., Ogawa K. 2008b. Philometra sawara sp. n. and a redescription of Philometra sciaenae Yamaguti, 1945 and Philometra nemipteri Luo, 2001 (Nematoda: Philometridae): a morphological and molecular approach. Folia Parasitologica, 55, 277–290.PubMedGoogle Scholar
  40. Quiazon K.M.A., Yoshinaga T., Ogawa K. 2010. Taxonomic note on the redescription of Philometroides seriolae (Ishii, 1931) infecting Japanese amberjack (Seriola quinqueradiata Temminck et Schlegel, 1845) in Japan. Journal of Tropical Biology, 8, 29–34.Google Scholar
  41. Radhakrishnan S., Beevi A.M., Deepthi G.R., Radhakrishnan T. 2010. Philometra cephalus (Nematoda) infection in the gonads of the long-arm mullet Valamugil cunnesius: host-parasite relation. Indian Journal of Fisheries, 57, 33–36.Google Scholar
  42. Sakaguchi S., Shibahara T., Yamagata Y. 1987. Parasitic ecology of a Philometra lateolabracis parasite on the red sea bream. Bulletin of National Research Institute of Aquaculture, 12, 73–78. (In Japanese, English summary).Google Scholar
  43. Séguin G., Bouchard F., Measures L.N., Uhland C.F., Lair S. 2011. Infections with Philometra sp. associated with mortalities in wild-hatched captive-raised striped bass, Morone saxatilis (Walbaum). Journal of Fish Diseases, 34, 475–481.PubMedCrossRefGoogle Scholar
  44. Schmidt G.D., Kuntz R.E. 1969. Nematode parasites of Oceanica. V. Four new species from fishes of Palawan, P. I., with a proposal for Oceanicucullanus gen. nov. Parasitology, 59, 389–396.PubMedCrossRefGoogle Scholar
  45. Tamura K., Dudley J., Nei M., Kumar S. 2007. Molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24, 1596–1599.PubMedCrossRefGoogle Scholar
  46. Wijová M., Moravec F., Horák A., Lukeš J. 2006. Evolutionary relationships of Spirurina (Nematoda: Chromadorea: Rhabditida) with special emphasis on dracunculoid nematodes inferred from SSU rRNA gene sequences. International Journal for Parasitology, 36, 1067–1075.PubMedCrossRefGoogle Scholar
  47. Zischke M.T., Cribb T.H., Welch D.J., Sawynok W., Lester R.J.G. 2009. Stock structure of blue threadfin Eleutheronema tetradactylum on the Queensland east coast, as determined by parasites and conventional tagging. Journal of Fish Biology, 75, 156–171.PubMedCrossRefGoogle Scholar

Copyright information

© Versita Warsaw and Springer-Verlag Wien 2013

Authors and Affiliations

  1. 1.Freshwater Aquaculture Center and College of FisheriesCentral Luzon State University, Science City of MuñozNueva EcijaPhilippines
  2. 2.Department of Aquatic Bioscience, Graduate School of Agricultural and Life SciencesThe University of TokyoTokyoJapan

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