Acta Parasitologica

, Volume 58, Issue 3, pp 376–383 | Cite as

Ancylostoma ceylanicum metalloprotease 6 DNA vaccination induces partial protection against hookworm challenge infection

  • Marcin Wiśniewski
  • Sławomir Jaros
  • Piotr Bąska
  • Michael Cappello
  • Halina Wędrychowicz
Original Paper

Abstract

Hookworms are blood feeding intestinal nematodes that infect more than 500 million people and cause iron deficiency anemia. Infected children suffer from physical and cognitive growth retardation. Because of potential anthelminthic drug resistance, the need for vaccine development is urgent. Numerous antigens have been tested in animal models as vaccines against hookworm infection, but there is no effective human vaccine. We cloned a cDNA encoding Ancylostoma ceylanicum metalloprotease 6 (Acemep-6). Ace-MEP-6 is a protein with a predicted molecular mass of 101.87 kDa and based on computational analysis it is very likely to be engaged in food processing via hemoglobin digestion. Groups of hamsters were immunized with an Ace-mep-6 cDNA vaccine, either once or three times. Animals that were administered one dose developed high resistance (80%, p < 0.01) against challenge infection, whereas triple immunization resulted in no worm burden reduction. These results suggest that DNA vaccines can be powerful tools in ancylostomiasis control, although the mechanisms through which protection is conferred remain unclear.

Keywords

Hookworm Ancylostoma metalloprotease DNA vaccination 

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Copyright information

© Versita Warsaw and Springer-Verlag Wien 2013

Authors and Affiliations

  • Marcin Wiśniewski
    • 1
  • Sławomir Jaros
    • 1
    • 2
  • Piotr Bąska
    • 3
  • Michael Cappello
    • 4
  • Halina Wędrychowicz
    • 1
    • 5
  1. 1.Division of Parasitology and Parasitic Diseases, Department of Preclinical Sciences, Faculty of Veterinary MedicineWarsaw University of Life SciencesWarsawPoland
  2. 2.Laboratory of Molecular Biology, Mabion Ltd.ŁódzPoland
  3. 3.Division of Pharmacology and Toxicology, Department of Preclinical Sciences, Faculty of Veterinary MedicineWarsaw University of Life SciencesWarsawPoland
  4. 4.Program in International Child Health, Departments of Pediatrics, Microbial Pathogenesis, and Public HealthYale University School of MedicineNew HavenUSA
  5. 5.Laboratory of Molecular ParasitologyW. Stefański Institute of Parasitology PASWarsawPoland

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