Acta Parasitologica

, Volume 58, Issue 3, pp 304–308 | Cite as

Susceptibility of Acanthamoeba to multipurpose lens-cleaning solutions

  • Ana Paula Costa de Aguiar
  • Caroline de Oliveira Silveira
  • Mari Aline Todero Winck
  • Marilise Brittes Rott
Original Paper


The present study investigated the susceptibility of Acanthamoeba spp. trophozoites to two multipurpose systems for cleaning and maintenance of contact lenses. Three strains of trophozoites from the ATCC (A. castellani T4, A. castellani Neff, and A. polyphaga) and two Acanthamoeba isolates obtained from swimming pools (PT5 and PO1) were placed in monoxenic culture. To test their survival in cleaning solutions for contact lenses, the trophozoites were exposed for 4 and 24 h to two multipurpose solutions (A and B), and were then inoculated into a new monoxenic culture. Amoebic growth on the plates was observed after 72 h of incubation. Trophozoites from all three ATCC strains and one isolate from a swimming pool (PO1) grew in all plates after 4 h of exposure to solutions A and B. After 24 h, the ATCC strains and the PO1 isolate showed growth in most of the plates treated. Only the PT5 isolate showed susceptibility to both solutions over the time intervals tested. The two solutions were not completely effective against most strains and isolates over the time intervals tested. These results are important, since species of Acanthamoeba are widely distributed in the environment and are potential agents of eye pathologies.


Acanthamoeba multipurpose solutions keratitis 


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  1. Booton G.C., Visvesvara G.S., Byers T.J., Byers T.J., Kelly D.J., Fuerst P.A. 2005. Identification and distribution of Acanthamoeba species genotypes associated with nonkeratitis infections. Journal of Clinical Microbiology, 43, 1689–1693. DOI: 10.1128/JCM.43.4.1689-1693.2005.PubMedCrossRefGoogle Scholar
  2. BRASIL, 2011. Agência Nacional de Vigilancia Sanitária. Serviçp de Registro de Produtos para Saúde [ANVISA web site]. Available in: Accessed November 7, 2010.Google Scholar
  3. Bryan K., Bugant J., Chang T., Chen S., Rosenberg J., Hammond R., McConnell K., Sanderson R., Nakata M., Wakida C., Austin C., Bestudik J., Bordson M.G., Conover C., Granzow L., Rea V., Chu A., Luckman E., Signs K., Harper J., Damrow T., Mosher E., Kruger K., Saheli E., Cassidy M., Hatch J., Weltman A., Garcia Rivera E.J., Garcia Y., Kainer M.A., Archer J., Joslin C., Cernoch P., Jones D., Hamill M., Matoba A., Pflugfelder S., Wilhelmus K., Beavers S., Chen T., Christian K., Cooper M., Dufficy D., Gershman M., Glenshaw M., Hall A., Holzbauer S., Huang A., Langer A., Moore Z., Patel A.S., Carpenter L.R., Schaffzin J., Su J., Trevino I., Weiser T., Wiersma P., Lorick S., Verani J.R. Acanthamoeba keratitis multiple states, 2005–2007 MMWR [CDC web site]. Available in: Accessed October 20, 2010.Google Scholar
  4. Carlesso A.M., Artuso G.L., Simonetti A.M., Rott M.B. 2007. Isolation and identification of potentially pathogenic free-living amoebae in samples from environments in a public hospital in the city of Porto Alegre, Rio Grande do Sul. Revista da Sociedade Brasileira de Medicina Tropical, 40, 316–320. DOI: 10.1590/S0037-86822007000300013.PubMedCrossRefGoogle Scholar
  5. Caumo K., Frasson A.P., Pens C.J., Panatieri L.F., Frazzon A.P., Rott M.B. 2009. Potentially pathogenic Acanthamoeba in swimming pools: a survey in the southern Brazilian city of Porto Alegre. Annals of Tropical Medicine and Parasitology, 103, 477–485. DOI: 10.1179/136485909X451825.PubMedCrossRefGoogle Scholar
  6. Caumo K., Rott MB. 2011. Acanthamoeba T3, T4 and T5 in swimming-pool waters from Southern Brazil. Acta Tropica, 117, 233–235. DOI: 10.1016/j.actatropica.2010.12.008.PubMedCrossRefGoogle Scholar
  7. Cohen E.J., Buchanan H.W., Laughrea P.A., Adams C.P., Galentine P.G., Visvesvara G.S., Folberg R., Arentsen J.J., Laibson P.R. 1985. Diagnosis and management of Acanthamoeba keratitis. American Journal of Ophthalmology, 100, 389–395.PubMedGoogle Scholar
  8. Corsaro D., Venditti D. 2010. Phylogenetic evidence for a new genotype of Acanthamoeba (Amoebozoa, Acanthamoebida). Parasitology Research, 107, 233–238. DOI: 10.1007/s00436-010-1870-6.PubMedCrossRefGoogle Scholar
  9. De Carli G.A., Moura H. 2007. Amebas de Vida Livre. In: De Carli GA. 2nd Ed. Parasitologia Clínica, Seleção de Métodos e Técnicas de Laboratório para o Diagnóstico das Parasitoses Humanas. Atheneu, Rio de Janeiro, 417–427.Google Scholar
  10. De Jonckheere J.F. 2003. Epidemiological typing of Acanthamoeba strains isolated from keratitis cases in Belgium. Bull Societ Belge Ophtalmology, 287, 27–33.Google Scholar
  11. Gast R.J. 2001. Development of Acanthamoeba-specific reverse dotblot and the discovery of a new ribotype. Journal of Eukaryotic Microbiology, 48, 609–615.PubMedCrossRefGoogle Scholar
  12. Hiti K., Walochnik J., Haller-Schober E.M., Faschinger C., Aspöck H. 2002. Viability of Acanthamoeba after exposure to a multipurpose disinfecting contact lens solution and two hydrogen peroxide systems. British Journal of Ophthalmology, 86, 144–146. DOI: 10.1136/bjo.86.2.144.PubMedCrossRefGoogle Scholar
  13. Iovieno A., Oechsler R.A., Ledee D.R., Miller D., Alfonso E.C. 2010. Drug-resistant severe Acanthamoeba keratitis caused by rare T5 Acanthamoeba genotype. Eye & Contact Lens, 36, 183–184. DOI: 10.1097/ICL.0b013e3181da2350.CrossRefGoogle Scholar
  14. Khan N.A., Jarrol E.L., Paget T.A. 2002. Molecular and physiological differentiation between pathogenic and nonpathogenic Acanthamoeba. Current Microbiology, 45, 197–202. DOI: 10.1007/s00284-001-0108-3.PubMedCrossRefGoogle Scholar
  15. Lipener C., Ray C.B. 2008. Sistemas atuais de cuidado e manutenção de lentes de contato. Arquivos Brasileiros de Oftalmologia, 71, 9–13.PubMedCrossRefGoogle Scholar
  16. Maghsood A.H., Sissons J., Rezaian M., Nolder D., Warhurst D., Khan N.A. 2005. Acanthamoeba genotypes T4 from the UK and Iran and isolation of the T2 genotype from clinical isolates. Journal of Medical Microbiology, 74, 755–759. DOI: 10.1099/jmm.0.45970-0.CrossRefGoogle Scholar
  17. Marciano-Cabral, Cabral G. 2003. Acanthamoeba spp. as agents of diseases in humans. Clinical Microbiology Reviews, 16, 273–307. DOI: 10.1128/CMR.16.2.273-307.2003.PubMedCrossRefGoogle Scholar
  18. McClellan K., Coster D.J. 1987. Acanthamoebic keratitis diagnosed by paracentesis and biopsy and treated with propamidine. British Journal of Ophthalmology, 71, 734–736.PubMedCrossRefGoogle Scholar
  19. Molmeret M., Horn M., Wagner M., Santic M., Abu Kwaik Y. 2005. Amoebae as training grounds for intracellular bacterials pathogens. Applied and Environmental Microbiology, 71, 20–28. DOI: 10.1128/AEM.71.1.20-28.2005.PubMedCrossRefGoogle Scholar
  20. Morlet N., Duguid G., Radford C., Matheson M., Dart J. 1997. Incidence of Acanthamoeba keratitis associated with contact lens wear. Lancet, 350, 414–416.PubMedCrossRefGoogle Scholar
  21. Patel A., Hammersmith K. 2008. Contact lens-related microbial keratitis: recent outbreaks. Current Opinion in Ophthalmology, 19, 302–306. DOI: 10.1097/ICU.0b013e3283045e74.PubMedCrossRefGoogle Scholar
  22. Pens C.J., da Costa M., Fadanelli C., Caumo K., Rott M. 2008. Acanthamoeba spp. and bacterial contamination in contact lens storage cases and the relationship to user profiles. Parasitology Research, 103, 1241–1245. DOI: 10.1007/s00436-008-1120-3.PubMedCrossRefGoogle Scholar
  23. Radfort C.F., Minassian D.C., Dart J.K.G. 2002. Acanthamoeba keratitis in England and Wales: incidence, outcome, and risks factors. Britsh Journal of Ophthalmology, 86, 536–542. DOI: 10.1136/bjo.86.5.536.CrossRefGoogle Scholar
  24. Schaumberg D.A., Snow K.K., Dana M.R. 1998. The epidemic of Acanthamoeba keratitis: where do we stand? Cornea, 17, 3–10.PubMedCrossRefGoogle Scholar
  25. Shoff M., Rogerson A., Schatz S., Seal D. 2007.Variable responses of Acanthamoeba strains to three multipurpose lens cleaning solutions. Investigative Ophthalmology & Visual Science, 84, 202–207. DOI: 10.1097/OPX.0b013e3180339f81.Google Scholar
  26. Shoff M.E., Joslin C.E., Tu E.Y., Kubatko L., Fuerst P.A. 2008. Efficacy of contact lens systems against recent clinical and tap water Acanthamoeba isolates. Cornea, 27, 713–719. DOI: 10.1097/QAI.0b013e31815e7251.PubMedGoogle Scholar
  27. Spanakos G., Tzanetou K., Miltsakakis D., Patsoula E., Malamou-Lada E., Vakalis N.C. 2006. Genotyping of pathogenic Acanthamoeba isolated from clinical samples in Greece — report of a clinical isolate presenting T5 genotype. Parasitology International, 55, 147–149. DOI: 10.1016/j.parint.2005.12.001.PubMedCrossRefGoogle Scholar
  28. Stothard D.R., Schroeder-Diedrich J.M., Awwad M.H., Gast R.J., Ledee D.R., Rodriguez-Zaragoza S., Dean C.L., Fuerst P.A., Byers T.J. 1998. The evolutionary history of the genus Acanthamoeba and identification of eight new 18S rRNA gene sequence types. Journal of Eukaryotic Microbiology, 45, 45–54. DOI: 10.1111/j.1550-7408.1998.tb05068.x.PubMedCrossRefGoogle Scholar
  29. Theodore F.H., Jakobiec F.A., Juechter K.B., Ma P., Troutman R.C., Panq P.M., Iwamoto T. 1985. The diagnostic value of a ring infiltrate in acanthamoebic keratitis. Ophthalmology, 2, 1471–1479.Google Scholar
  30. Walochnick J., Obwaller A., Aspock H. 2000. Correlations between morphological, molecular, biological and physiological characteristics in clinical and nonclinical isolates of Acanthamoeba spp. Applied and Environmental Microbiology, 66, 4408–4413. DOI: 10.1128/AEM.66.10.4408-4413.2000.CrossRefGoogle Scholar
  31. Yu H.S., Kong H.H., Kim S.Y., Hahn Y.H., Hahn T.W., Chung D.I. 2004. Laboratory investigation of Acanthamoeba lugdunensis from patients with keratitis. Investigative Ophthalmology & Visual Science, 45, 1418–1426. DOI: 10.1167/iovs.03-0433.CrossRefGoogle Scholar
  32. Zheng X., Uno T., Goto T., Zhang W., Hill J.H. 2004. Pathogenic Acanthamoeba Induces Apoptosis of Human Corneal Epithelial cells. Japanese Journal of Ophthalmology, 48, 23–29. DOI: 10.1007/s10384-003-0018-y.PubMedCrossRefGoogle Scholar

Copyright information

© Versita Warsaw and Springer-Verlag Wien 2013

Authors and Affiliations

  • Ana Paula Costa de Aguiar
    • 1
  • Caroline de Oliveira Silveira
    • 1
  • Mari Aline Todero Winck
    • 1
  • Marilise Brittes Rott
    • 1
  1. 1.Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da SaúdeUniversidade Federal do Rio Grande do SulPorto Alegre, RSBrazil

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