Acta Parasitologica

, Volume 58, Issue 2, pp 127–131 | Cite as

Metabolomics and protozoan parasites

  • Timothy Paget
  • Nicolas Haroune
  • Sushmita Bagchi
  • Edward Jarroll
Invited Article

Abstract

In this review, we examine the state-of-the-art technologies (gas and liquid chromatography, mass spectroscopy and nuclear magnetic resonance, etc.) in the well-established area of metabolomics especially as they relate to protozoan parasites.

Keywords

Metabolomics protists nuclear magnetic resonance gas and liquid chromatography mass spectroscopy 

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References

  1. Baker M. 2013. The ‘omes’ puzzle. Nature, 494, 416–419. DOI: 10.1038/494416a.PubMedCrossRefGoogle Scholar
  2. Besteiro S., VoDuy S., Perigaud C., Lefebvre-Tournier I., Vial H. 2010. Exploring metabolomic approaches to analyze phospholipid biosynthetic pathways in Plasmodium. Parasitology, 137, 1343–1356. DOI: http://dx.doi.org/10.1017/S0031182009991934.PubMedCrossRefGoogle Scholar
  3. Brauer M., Yuan J., Bennett B., Lu W., Kimball E., Botstein D., Rabinowitz J. 2006. Conservation of the metabolomic response to starvation across two divergent microbes. Proceedings of National Academy of Science of United States of America, 103, 19302–19307. DOI: 10.1073/pnas.0609508103.CrossRefGoogle Scholar
  4. Cho K., Shibato J., Agrawal G., Jung Y., Kubo A., Jwa N., Tamogami S., Satoh K., Kikuchi S., Higashi T., Kimura S., Saji H., Tanaka Y., Iwahashi H., Masuo Y., Rakwal R. 2008. Integrated transcriptomics, proteomics and metabolomics analyses to survey ozone responses in the leaves of rice seedling. Journal of Proteome Research, 7, 2980–2998. DOI: 10.1021/pr800128q.PubMedCrossRefGoogle Scholar
  5. Creek D., Anderson J., McConville M., Barrett M. 2012. Metabolomic analysis of trypanosomatid protozoa. Molecular and Biochemical Parasitology, 181, 73–84. DOI: org/10.1016/j.molbiopara.2011.10.003.PubMedCrossRefGoogle Scholar
  6. Holmes E. 2010. The evolution of metabolic profiling in parasitology. Parasitology, 137, 1437–1449. DOI:http://dx.doi.org/10.1017/S0031182010000260.PubMedCrossRefGoogle Scholar
  7. Husain A., Sato D., Jeelani G., Mi-ichi F., Ali V., Suematsu M., Soga T., Nozaki T. 2010. Metabolome analyses revealed increase in S-methylcysteine and phosphatidylisopropanolamine synthesis upon L-cysteine deprivation in the anaerobic protozoan parasite Entamoeba histolytica. Journal of Biological Chemistry, 285, 39160–39170. DOI: 10.1074/jbc.M110.167304.PubMedCrossRefGoogle Scholar
  8. Jeelani G., Sato D., Husain A., Escueta-de Cadiz A., Sugimoto M. Soga T., Suematsu M., Nozaki T. 2012. Metabolic profiling of the protozoan parasite Entamoeba invadens revealed activation of unpredicted pathway during encystation. PLoS ONE, 7, e37740. DOI: 10.1371/journal.pone.0037740.PubMedCrossRefGoogle Scholar
  9. Kafsack B., Llinás M. 2010. Eating at the table of another: metabolomics of host-parasite interactions. Cell Host & Microbe, 7, 90–99. DOI: http://dx.doi.org/10.1016/j.chom.2010.01.008.CrossRefGoogle Scholar
  10. Khoo S., Al-Rubeai M. 2007. Metabolomics as a complementary tool in cell culture. Biotechnology and Applied Biochemistry, 47, 71–84. DO:10.1042/BA20060221.PubMedCrossRefGoogle Scholar
  11. Lakshmanan V., Rhee K., Daily J. 2011. Metabolomics and malaria biology. Molecular and Biochemical Parasitology, 175, 104–111. DOI: http://dx.doi.org/10.1016/j.molbiop2012.PubMedCrossRefGoogle Scholar
  12. Lakshmanan V., Rhee K., Wang W., Yu Y., Khafizov K., Fiser A., Wu P., Ndir O., Mboup S., Ndiaye D., Daily J. 2012. Metabolomic analysis of patient plasma yields evidence of plant-likelinolenic acid metabolism in Plasmodium falciparum. Journal of Infectious Diseases, 206, 238–248. DOI: 10.1093/infdis/jis339.PubMedCrossRefGoogle Scholar
  13. Lindmark D., van Keulen H., Paget T., Jarroll E. 2011. Giardia metabolism. In: S. Svärd and H. Luján, (Eds.), Giardia. New York, Springer, Chapter 8, 127–137.Google Scholar
  14. Lisec J., Meyer R., Steinfath M., Redestig H., Becher M., Witucka-Wall H., Fiehn O., Törjék O., Selbig J., Altmann T., Willmitzer L. 2008. Identification of metabolic and biomass QTL in Arabidopsis thaliana in a parallel analysis of RIL and IL populations. Plant Journal: for Cell and Molecular Biology, 53, 960–972. DOI: 10.1111/j.1365-313X.2007.03383.x.CrossRefGoogle Scholar
  15. Maier K., Hofmann U., Reuss M., Mauch K. 2008. Identification of metabolic fluxes in hepatic cells from transient 13C-labeling experiments: Part II. Flux estimation. Biotechnology and Bioengineering, 100, 355–370. DOI: 10.1002/bit.21746.PubMedCrossRefGoogle Scholar
  16. Meng Q., Mäkinen V.P., Luk H., Yang X. 2013. Systems Biology Approaches and Applications in Obesity, Diabetes, and Cardiovascular Diseases. Current Cardiovascular Risk Report, 7, 73–83. DOI: 10.1007/s12170-012-0280-yCrossRefGoogle Scholar
  17. Nevedomskaya E., Mayboroda O., Deelder A. 2011. Cross-platform analysis of longitudinal data in metabolomics. Molecular Biosystems, 7, 3214–3222. DOI: 10.1039/C1MB05280B.PubMedCrossRefGoogle Scholar
  18. Pedersen K., Kristensen T., Loeschcke V., Petersen B.O., Duus J., Nielsen N., Malmendal A. 2008. Metabolomic signatures of inbreeding at benign and stressful temperatures in Drosophila melanogaster. Genetics, 180, 1233–1243. DOI: 10.1534/genetics.108.089144.PubMedCrossRefGoogle Scholar
  19. Rabinowitz J. 2007. Cellular metabolomics of Escherichia coli. Expert Review of Proteomics, 4, 187–198. DOI: 10.1586/14789450.4.2.187.PubMedCrossRefGoogle Scholar
  20. Russell C., Rahman A., Mohammed R. 2013. Application of genomics, proteomics and metabolomics in drug discovery, development and clinic. Therapeutic Delivery, 4, 395–413. DOI: 10.4155/tde.13.4.PubMedCrossRefGoogle Scholar
  21. Scheltema R., Decuypere S., T’Kindt R., Dujardin J., Coombs G., Breitling R. 2010. The potential of metabolomics for Leishmania research in the post-genomics era. Parasitology, 137, 1291–1302. DOI: http://dx.doi.org/10.1017/S0031182009992022.PubMedCrossRefGoogle Scholar
  22. Shulaev V. 2006. Metabolomics technology and bioinformatics. Briefings in Bioinformatics, 7, 128–39. DOI: 10.1093/bib/bbl012.PubMedCrossRefGoogle Scholar
  23. Sun G., Yang K., Zhao Z., Guan S., Han X., Gross R. 2007. Shotgun metabolomics approach for the analysis of negatively charged water — soluble cellular metabolites from mouse heart tissue. Analytical Chemistry, 79, 6629–6640. DOI: 10.1021/ac07 0843+PubMedCrossRefGoogle Scholar
  24. Trauger S., Kalisak E., Kalisiak J., Morita H., Weinberg M., Menon A., Poole F. Adams M., Siuzdak G. 2008. Correlating the transcriptome, proteome and metabolome in the environmental adaptation of hyperthermophile. Journal of Proteome Research, 7, 1027–1035. DOI: 10.1021/pr700609.PubMedCrossRefGoogle Scholar
  25. Vincent I., Creek D., Burgess K., Woods D., Burchmore R., Barrett M. 2012. Untargeted metabolomics reveals a lack of synergy between nifurtimox and eflornithine against Trypanosoma brucei. PLoS Neglected Tropical Disease, 6, e1618. DOI: 10.1371/journal.pntd.0001618.CrossRefGoogle Scholar

Copyright information

© Versita Warsaw and Springer-Verlag Wien 2013

Authors and Affiliations

  • Timothy Paget
    • 1
    • 2
  • Nicolas Haroune
    • 2
  • Sushmita Bagchi
    • 1
  • Edward Jarroll
    • 3
  1. 1.Department of Chemistry, Lehman CollegeCity University of New YorkBronxUSA
  2. 2.Sunderland School of PharmacyUniversity of SunderlandSunderlandUK
  3. 3.Department of Biological Sciences, D-130, Lehman CollegeCUNYBronxUSA

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