Acta Parasitologica

, Volume 58, Issue 2, pp 218–222 | Cite as

Detection of Theileria equi in spleen and blood of asymptomatic piroplasm carrier horses

  • Isabel B. Ribeiro
  • Antônio Carlos L. Câmara
  • Marta V. Bittencourt
  • Tatiana G. Marçola
  • Giane R. Paludo
  • Benito Soto-Blanco
Research Note
  • 258 Downloads

Abstract

This study aimed to determine whether asymptomatic horses naturally infected with Theileria equi retain infected erythrocytes in the spleen and whether the presence of the hemoparasite in this organ is associated with parasitemia. We collected samples from 25 adult horses without clinical signs of any disease. From each animal, we collected whole blood samples from the jugular vein and a splenic puncture blood sample. All samples were submited to blood cell counts and detection of Theileria or Babesia. DNA extraction and PCR were performed in all samples for identification of piroplasm infection (T. equi and B. caballi). From the 25 horses evaluated for piroplasm detection by PCR, seven horses (28%) were positive in jugular vein blood but negative in splenic blood samples, five horses (20%) were positive in splenic blood samples but negative in jugular vein blood samples, and 13 horses (52%) were positive in both jugular vein and splenic blood samples. The hematological evaluation revealed anemia in 13 of 25 (52%) infected horses, lymphopenia in five (20%), neutrophilia in two (8%), neutropenia in one (4%), and thrombocytopenia in one (4%) infected horse. The present study demonstrated that several (20%) of the asymptomatic piroplasm carrier horses did not show parasitemia, but show infected erythrocytes in the spleen.

Keywords

Theileria equi Babesia caballi hemoparasites piroplasm carrier parasitemia 

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Copyright information

© Versita Warsaw and Springer-Verlag Wien 2013

Authors and Affiliations

  • Isabel B. Ribeiro
    • 1
  • Antônio Carlos L. Câmara
    • 1
  • Marta V. Bittencourt
    • 2
  • Tatiana G. Marçola
    • 2
  • Giane R. Paludo
    • 2
  • Benito Soto-Blanco
    • 1
    • 3
  1. 1.Programa de Pós-Graduação em Ciência AnimalUniversidade Federal Rural do Semi-árido (UFERSA)MossoróBrazil
  2. 2.Laboratório de Patologia Clínica Veterinária, Faculdade de Agronomia e Medicina VeterináriaUniversidade de Brasília (UnB), Campus Universitário Darcy Ribeiro, ICC SulAsa Norte, BrasíliaBrazil
  3. 3.Departamento de Clínica e Cirurgia Veterinárias, Escola de VeterináriaUniversidade Federal de Minas GeraisBelo HorizonteBrazil

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