Excretory/secretory products of Fasciola hepatica but not recombinant phosphoglycerate kinase induce death of human hepatocyte cells
The liver fluke Fasciola hepatica infects a wide range of hosts, and has a considerable impact on the agriculture industry, mainly through infections of sheep and cattle. Further, human infection is now considered of public health importance and is hyperendemic in some regions. The fluke infection causes considerable damage to the hosts’ liver. However, the mechanisms of liver destruction have not yet been completely elucidated. In the present report we incubated a human liver cell line in the presence of either F. hepatica excretory/secretory material (FhES) or recombinant phosphoglycerate kinase (FhPGK). Dosedependent cytotoxicity in the presence of FhES was observed, indicating that FhES is capable of killing human hepatocytes, supporting a role for FhES in damaging host liver cells during infection; while treatment with a recombinant intracellular protein — FhPGK, had no impact on cell survival.
KeywordsHuman hepatocyte F. hepatica ES recombinant FhPGK
Unable to display preview. Download preview PDF.
- Behm C.A., Sangster N.C. 1999. Pathology, pathophysiology and clinical aspects. In: (Ed. J.P. Dalton) Fasciolosis. CABI Publishing, Wallingford, 185–224.Google Scholar
- Gajewska A., Smaga-Kozlowska K., Kotomski G. 2006. Effect of excretory-secretory products of Fasciola hepatica on the functioning of rat hepatocytes. Medycyna Weterynaryjna, 62, 459–462.Google Scholar
- Morphew R.M., Wright H.A., LaCourse E.J., Woods D.J., Brophy P.M. 2007. Comparative proteomics of excretory-secretory proteins released by the liver fluke Fasciola hepatica in sheep host bile and during in vitro culture ex host. Molecular & Cellular Proteomics: MCP, 6, 963–972. DOI: 10.1074/mcp.M600375-MCP200.CrossRefGoogle Scholar
- Robinson M.W., Menon R., Donnelly S.M., Dalton J.P., Ranganathan S. 2009. An integrated transcriptomics and proteomics analysis of the secretome of the helminth pathogen Fasciola hepatica: proteins associated with invasion and infection of the mammalian host. Molecular & Cellular Proteomics: MCP, 8, 1891–1907. DOI: 10.1074/mcp.M900045-MCP200.CrossRefGoogle Scholar
- Serradell M.C., Guasconi L., Masih D.T. 2009. Involvement of a mitochondrial pathway and key role of hydrogen peroxide during eosinophil apoptosis induced by excretory-secretory products from Fasciola hepatica. Molecular and Biochemical Parasitology, 163, 95–106. DOI: 10.1016/j.molbiopara.2008.10.005.PubMedCrossRefGoogle Scholar
- WHO. 1995. Control of foodborne trematode infections: report of a WHO study group. World Health Organization technical report series, 849, 1–157.Google Scholar
- Wilson R.A., Wright J.M., de Castro-Borges W., Parker-Manuel S.J., Dowle A.A., Ashton P.D., Young N.D., Gasser R.B., Spithill T.W. 2011. Exploring the Fasciola hepatica tegument proteome. International Journal for Parasitology, 41, 1347–1359. DOI: DOI 10.1016/j.ijpara.2011.08.003.PubMedCrossRefGoogle Scholar