Cell phenotypic change due to Cryptosporidium parvum infection in immunocompetent mice
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Cryptosporidium parvum is an intracellular parasite causing enteritis which can become life-threatening in immunocompromised host. Immunoregulatory T cells play a central role in the regulatory network of the host. Here, we proposed to characterize the populations of immune cells during infection and reinfection with C. parvum. Four-week-old BALB/C mice were inoculated with oocysts of C. parvum at days 0 and 22. Fecal and blood samples, spleens, and small intestines were collected for analysis. Peripheral blood and spleen cell populations were characterized by flow cytometry. After infection (days 0 to 21), mice presented higher values of neutrophils, eosinophils, NK cells and CD4+CD25high T cells in peripheral blood. After reinfection, this upward trend continued in the following days for all four populations in infected mice. At day 35, infected mice presented similar values to the control group, except for CD4+CD25high T cells, which remained higher in infected mice. A possible correlation between alterations in blood and spleen cell populations was also studied, but no consistent association could be established. Small intestine sections were screened for intracellular stages of the parasite but no evidence of pathology was observed. Here, we report information which may be important for the understanding of the specific cell-mediated response in immunocompetent mice to C. parvum infection. Although some questions remain unanswered and complementary studies are needed, our results are expected to contribute to a better understanding of innate and Treg cells role in the clearance process of this parasite.
KeywordsCryptosporidium immunophenotype flow cytometry B-lymphocytes T-lymphocytes
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- Borrego L.M., Arroz M.J., Videira P., Martins C., Guimarães H., Nunes G., Papoila A.L., Trindade H. 2009. Regulatory cells, cytokine pattern and clinical risk factors for asthma in infants and young children with recurrent wheeze. Clinical and Experimental Allergy, 39, 1160–1169. DOI: 10.1111/j.1365-2222.2009.03253.x.PubMedCrossRefGoogle Scholar
- Certad G., Ngouanesavanh T., Guyot K., Gantois N., Chassat T., Mouray A., Fleurisse L., Pinon A., Cailliez J.C., Dei-Cas E., Creusy C. 2007. Cryptosporidium parvum, a potential cause of colic adenocarcinoma. Infectious Agents and Cancer, 21, 2–22. DOI: 10.1186/1750-9378-2-22.Google Scholar
- Del Coco V.F., Córdoba M.A., Sidoti A., Santín M., Drut R., Basualdo J.A. 2012. Experimental infection with Cryptosporidium parvum IIaA21G1R1 subtype in immunosuppressed mice. Veterinary Parasitology, http://dx.doi.org/10.1016/j.vetpar.2012.06.033.
- Glaberman S., Moore J.E., Lowery C.J., Chalmers R.M., Sulaiman I., Elwin K., Rooney P.J., Millar B.C., Dooley J.S., Lal A.A., Xiao L. 2002. Three drinking-water-associated cryptosporidiosis outbreaks, Northern Ireland. Emerging Infectious Diseases, 8, 631–633. DOI: 10.3201/eid0806.010368.PubMedCrossRefGoogle Scholar
- Gomez Morales M.A., Mele R., Ludovisi A., Bruschi F., Tosini F., Pozio E. 2004. Cryptosporidium parvum-specific CD4 Th1 cells from sensitized donors responding to both fractionated and recombinant antigenic proteins. Infection and Immunity, 72, 1306–1310. DOI: 10.1128/IAI.72.3.1306-1310.2004.PubMedCrossRefGoogle Scholar
- Liu W., Putnam A.L., Xu-Yu Z., Szot G.L., Lee M.R., Zhu S., Gottlieb P.A., Kapranov P., Gingeras T.R., Fazekas de St Groth B., Clayberger C., Soper D.M., Ziegler S.F., Bluestone J.A. 2006. CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4+ Treg cells. Journal of Experimental Medicine, 203, 1701–1711. DOI: 10.1084/jem.20060772.PubMedCrossRefGoogle Scholar
- Mac Kenzie W.R., Hoxie N.J., Proctor M.E., Gradus M.S., Blair K.A., Peterson D.E., Kazmierczak J.J., Addiss D.G., Fox K.R., Rose J.B., Davis J.P. 1994. A massive outbreak in Milwaukee of Cryptosporidium infection transmitted through the public water supply. New England Journal of Medicine, 331, 161–167.PubMedCrossRefGoogle Scholar
- Male D., Brostoff J., Roth D.B., Roitt I. 2006. Immunity to protozoa and worms. In: (Ed. Mosby Elservier) Immunology. 7th. Canada, 277–297.Google Scholar
- Pinchuk L.M., Filipov N.M. 2008. Differential effects of age on circulating and splenic leukocyte populations in C57BL/6 and BALB/c male mice. Immunity and Ageing, 11, 5–11. DOI: 10.1186/1742-4933-5-1.Google Scholar
- Xiao L., Ryan U.M. 2008. Molecular epidemiology. In: (Eds. R. Fayer and L. Xiao) Cryptosporidium and Cryptosporidiosis. CRC Press and IWA Publishing, Boca Raton, FL, 119–171.Google Scholar