Acta Parasitologica

, Volume 58, Issue 1, pp 13–17 | Cite as

First detection and genotyping of human-associated microsporidia in wild waterfowl of Slovakia

  • B. Malčeková
  • A. ValenčákováEmail author
  • L. Molnár
  • A. Kočišová
Original Paper


A total of 47 avian faecal samples of wild waterfowl (great cormorant — Phalacrocorax carbo, great crested grebe — Podiceps cristatus, white stork — Ciconia ciconia) trapped in the eastern Slovakia were screened for the presence of human pathogenic microsporidia by microscopy and real-time SYBR Green PCR method using species primers and sequenced. Microscopic analysis showed presence in 32 samples (29 cormorants, 3 dippers). Microsporidial DNA (Encephalitozoon cuniculi genotype I) was identified in 19 faeces samples (40.4%) namely cormorants in 17 out of 40, one dipper of 5 and a stork out of 2. The present work describes three new host species of the bird population in microsporidium Encephalitozoon cuniculi genotype I which confirms the theory of low specificity of this species.


Waterfowl SYBR Green PCR microscopic method Encephalitozoon cuniculi genotype I 


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Copyright information

© Versita Warsaw and Springer-Verlag Wien 2013

Authors and Affiliations

  • B. Malčeková
    • 1
  • A. Valenčáková
    • 1
    Email author
  • L. Molnár
    • 2
  • A. Kočišová
    • 3
  1. 1.Department of Biology and GeneticsUniversity of Veterinary Medicine and Pharmacy in KošiceKošiceSlovakia
  2. 2.Avian and Exotic ClinicUniversity of Veterinary Medicine and Pharmacy in KošiceKošiceSlovakia
  3. 3.Department of Infectious Disease and EpizootologyUniversity of Veterinary Medicine and Pharmacy in KošiceKošiceSlovakia

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