Abstract
The impact of in vitro isolation and molecular characterisation of Neospora caninum as well as sequence analyses was studied. The brain homogenate of a naturally Neospora-infected dairy cow (positive in ELISA and Western blot) was intraperitoneally inoculated into Mongolian gerbils (Meriones unguiculatus). The brain of gerbils on day 60 post-inoculation was homogenized, and, after trypsin-digestion, cultured on Vero cells. Neospora-like tachyzoites were first observed after 77 days of cultivation. The parasite was confirmed by polymerase chain reaction (PCR) using Neospora-specific primers Np21 and Np6. The PCR product of the first Slovak isolate (NC-SKB1) was subsequently sequenced and published in GenBank under accession number GU300774. Sequencing and BLAST search identified the isolate as N. caninum.
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Reiterová, K., Špilovská, S., Čobádiová, A. et al. First in vitro isolation of Neospora caninum from a naturally infected adult dairy cow in Slovakia. Acta Parasit. 56, 111–115 (2011). https://doi.org/10.2478/s11686-011-0019-9
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DOI: https://doi.org/10.2478/s11686-011-0019-9