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Ultrastructure of the spermatozoon of Calliobothrium verticillatum (Cestoda, Tetraphyllidea, Oncobothriidae)

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Abstract

The ultrastructure of the spermatozoon of Calliobothrium verticillatum (Cestoda, Tetraphyllidea, Oncobothriidae) parasite of the smoothhound shark, Mustelus mustelus L. (Pisces, Carcharhiniformes), was studied by transmission electron microscopy. This spermatozoon presents five regions characterized by several ultrastructural elements: an apical cone, a crested body, two axonemes of 9 + “1” pattern, electron-dense granules, a nucleus and cortical microtubules. In the present study, three of these features were the subject of a detailed attention. The first is the presence of two axonemes, which confirms that the Tetraphyllidea, Oncobothriidae possess two axonemes whereas the Tetraphyllidea, Phyllobothriidae possess only one axoneme. The second is the presence of one crested body, a criterion homogeneous in the Tetraphyllidea but heterogeneous among the different orders of Cestoda. The third is the number and the disposition of cortical microtubules. These three criteria seem to be interesting for phylogeny.

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References

  • Bâ A., Bâ C.T., Marchand B. 2000. Ultrastructure of spermiogenesis and the spermatozoon of Sudarikovina taterae (Cestoda, Cyclophyllidea, Anoplocephalidae) intestinal parasite of Tatera gambiana (Rodentia, Gerbillidae). Journal of Submicroscopic Cytology and Pathology, 32, 137–144.

    PubMed  Google Scholar 

  • Bâ A., Bâ C.T., Marchand B. 2002. Ultrastructural study of the spermatozoon of Echinocotyle dolosa (Cestoda, Cyclophyllidea, Hymenolepididae). Acta Parasitologica, 47, 131–136.

    Google Scholar 

  • Bâ C.T., Bâ A., Marchand B. 2005. Ultrastructure of the spermatozoon of Paroniella reynoldsae (Cyclophyllidea, Davaineidae) an intestinal parasite of Corvus albus (Aves, Corvidae). Acta Parasitologica, 50, 208–214.

    Google Scholar 

  • Bâ C.T., Marchand B. 1992a. Reinvestigation of the ultrastructure and the spermatozoon of Hymenolepis nana (Cestoda, Cyclophyllidea), parasite of the small intestine of Rattus rattus. Molecular Reproduction and Development, 33, 39–45. DOI: 10.1002/mrd.1080330106.

    Article  PubMed  Google Scholar 

  • Bâ C.T., Marchand B. 1992b. Ultrastructural particularities of the spermatozoon of Stilesia globipunctata (Cestoda) parasite of the small intestine of sheep and goats in Senegal. Journal of Submicroscopic Cytology and Pathology, 24, 29–34.

    Google Scholar 

  • Bâ C.T., Marchand B. 1994a. Similitude ultrastructurale des spermatozoïdes de quelques Cyclophyllidea. Parasite, 1, 51–55.

    PubMed  Google Scholar 

  • Bâ C.T., Marchand B. 1994b. Ultrastructure of spermiogenesis and the spermatozoon of Mathevotaenia herpestis (Cestoda), intestinal parasite of Atelerix albiventris in Senegal. Acta Zoologica, 75, 167–175.

    Article  Google Scholar 

  • Bâ C.T., Marchand B. 1994c. Ultrastructure of the spermatozoon of Sandonella sandoni (Cestoda, Proteocephalidea, Sandonellinae). Invertebrate Reproduction and Development, 25, 9–17.

    Google Scholar 

  • Bâ C.T., Marchand B. 1995. Spermiogenesis, spermatozoa and phyletic affinities in the Cestoda. In: (Eds. B.G.M. Jamieson, J. Ausio and J.-L. Justine) Advances in spermatozoal phylogeny and taxonomy. Mémoires du Muséum National d’Histoire Naturelle, 166, 87–95.

  • Bâ C.T., Marchand B., Mattei X. 1991. Demonstration of the orientation of the cestodes spermatozoon illustrated by the ultrastructural study of spermiogenesis of a Cyclophyllidea: Thysaniezia ovilla Rivolta, 1874. Journal of Submicroscopic Cytology and Pathology, 23, 605–612.

    PubMed  Google Scholar 

  • Brooks D.R. 1989. A summary of the database pertaining to the phylogeny of the major groups of the parasitic platyhelminths, with a revised classification. Canadian Journal of Zoology, 67, 714–720.

    Google Scholar 

  • Brooks D.R., O’Grady R.T., Glen D.R. 1985. The phylogeny of the Cercomeria Brooks, 1982 (Platyhelminthes). Proceedings of the Helminthological Society of Washington, 52, 1–20.

    Google Scholar 

  • Bruňanská M., Nebesářová J., Scholz T., Fagerholm H.P. 2002. Ultrastructure of the spermatozoon of the pseudophyllidean cestode Eubothrium crassum (Bloch, 1779). Parasitology Research, 88, 285–291. DOI: 10.1007/s00436-001-0532-0.

    Article  PubMed  Google Scholar 

  • Bruňanská M., Scholz T., Ibraheem M.H. 2004. Ultrastructural characters of the spermatozoon of the cestode Corallobothrium solidum Fritsch, 1886 (Cestoda: Proteocephalidea), a parasite of the electric catfish Malapterurus electricus. Parasitology Research, 94, 421–426. DOI: 10.1007/s00436-004-1236-z.

    Article  PubMed  Google Scholar 

  • Ehlers U. 1984. Phylogenetisches system der Plathelminthes. Verhandlungen des Naturwissenschaftlichen Verein in Hamburg (NF), 27, 291–294.

    Google Scholar 

  • Ehlers U. 1986. Comments on a phylogenetic system of the Platy-helminthes. Hydrobiologia, 132, 1–12. DOI: 10.1007/BF00046222.

    Article  Google Scholar 

  • Eira C., Miquel J., Vingada J., Torres J. 2006. Spermiogenesis and spermatozoon ultrastructure of the cestode Mosgovoyia ctenoides (Cyclophyllidea: Anoplocephalidae), an intestinal parasite of Oryctolagus cuniculus (Lagomorpha: Leporidae). Journal of Parasitology, 92, 708–718. DOI: 1645/GE-818R.1.

    Article  PubMed  Google Scholar 

  • Euzet L., Świderski Z., Mokhtar-Maamouri F. 1981. Ultrastructure comparée du spermatozoïde des cestodes. Relations avec la phylogénèse. Annales de Parasitologie Humaine et Comparée, 56, 247–259.

    PubMed  CAS  Google Scholar 

  • Justine J.-L. 1986. Ultrastructure of the spermatozoon of the cestode Duthiersia fimbriata (Pseudophyllidea, Diphyllobothriidae). Canadian Journal of Zoology, 64, 1545–1548.

    Article  Google Scholar 

  • Justine J.-L. 1991. Phylogeny of parasitic Platyhelminthes: a critical study of synapomorphies proposed on the basis of the ultrastructure of spermiogenesis and spermatozoa. Canadian Journal of Zoology, 69, 1421–1440.

    Article  Google Scholar 

  • Justine J.-L. 1997. La classification générale des Plathelminthes parasites: changements récents et utilisation des caractères ultrastructuraux, en particulier des spermatozoïdes. Bulletin de la Société Zoologique de France, 122, 269–277.

    Google Scholar 

  • Justine J.-L. 1998. Systématique des grands groupes de Plathelminthes parasites: quoi de neuf? Bulletin de la Société Française de Parasitologie, 16, 34–52.

    Google Scholar 

  • Levron C., Bruňanská M., Marchand B. 2005. Spermiogenesis and sperm ultrastructure of the pseudophyllidean cestode Triaenophorus nodulosus (Pallas, 1781). Parasitology Research, 98, 26–33. DOI: 10.1007/s00436-005-0009-7.

    Article  PubMed  Google Scholar 

  • Levron C., Bruňaská M., Poddubnaya L.G. 2006a. Spermatological characters in Diphyllobothrium latum (Cestoda, Pseudophyllidea). Journal of Morphology, 267, 1110–1119. DOI:10.1002/jmor.10460.

    Article  PubMed  Google Scholar 

  • Levron C., Bruňanská M., Kuchta R., Freeman M., Scholz T. 2006b. Spermatozoon ultrastructure of the pseudophyllidean cestode Paraechinophallus japonicusi, a parasite of deep-sea fish Psenopsis anomala (Perciformes, Centrolophidae). Parasitology Research, 100, 115–121. DOI: 10.1007/s00436-006-0224-x.

    Article  PubMed  Google Scholar 

  • Levron C., Bruňanská M., Poddubnaya L.G. 2006c. Spermatological characters of the pseudophyllidean cestode Bothriocephalus scorpii (Müller, 1776). Parasitology International, 55, 113–120. DOI: 10.1016/j.parint.2005.11.055.

    Article  PubMed  CAS  Google Scholar 

  • MacKinnon B.M., Burt M.D.B. 1984. The comparative ultrastructure of spermatozoa from Bothrimonus sturionis Duv. 1842 (Pseudophyllidea), Pseudanthobothrium hanseni Baer, 1956 (Tetraphyllidea), and Monoecocestus americanus Stiles, 1895 (Cyclophyllidea). Canadian Journal of Zoology, 62, 1059–1066.

    Article  Google Scholar 

  • Mahendrasingam S., Fairweather I., Halton D.W. 1989. Spermatogenesis and the fine structure of the mature spermatozoon in the free proglottis of Trilocularia acanthiaevulgaris (Cestoda, Tetraphyllidea). Parasitology Research, 75, 287–298. DOI: 10.1007/BF00931813.

    Article  PubMed  CAS  Google Scholar 

  • Miquel J., Feliu C., Marchand B. 1999. Ultrastructure of spermiogenesis and the spermatozoon of Mesocestoides litteratus (Cestoda, Mesocestoididae). International Journal for Parasitology, 29, 499–510. DOI: 10.1016/S0020-7519(98)00202-1.

    Article  PubMed  CAS  Google Scholar 

  • Miquel J., Marchand B. 1997. Ultrastructure of the spermatozoon of Dipylidium caninum (Cestoda, Cyclophyllidea, Dilepididae), intestinal parasite of Canis familiaris. Parasitology Research, 83, 349–355. DOI: 10.1007/s004360050260.

    Article  PubMed  CAS  Google Scholar 

  • Miquel J., Świderski Z., Marchand B. 2005. Spermatological characters in the Dipylidiidae Stiles, 1896 (Cestoda, Cyclophyllidea). Acta Parasitologica, 50, 65–73.

    Google Scholar 

  • Mokhtar-Maamouri F. 1979. Étude en microscopie électronique de la spermiogenèse et du spermatozoïde de Phyllobothrium gracile Wedl, 1855 (Cestoda, Tetraphyllidea, Phyllobothriidae). Zeitschrift für Parasitenkunde, 59, 245–258.

    Article  Google Scholar 

  • Mokhtar-Maamouri F. 1980. Particularités des processus de la fécondation chez Acanthobothrium filicolle Zschokke, 1888 (Cestoda: Tetraphyllidea, Onchobothriidae). Archives de l’Institut Pasteur de Tunis, 57, 191–205.

    PubMed  CAS  Google Scholar 

  • Mokhtar-Maamouri F. 1982. Étude ultrastructurale de la spermiogenèse de Acanthobothrium filicolle var. filicolle Zschokke, 1888 (Cestoda, Tetraphyllidea, Onchobothriidae). Annales de Parasitologie, 57, 429–442.

    CAS  Google Scholar 

  • Mokhtar-Maamouri F., Świderski Z. 1975. Étude en microscopie électronique de la spermatogénèse de deux cestodes Acanthobothrium filicolle benedenii Loennberg, 1889 et Onchobothrium uncinatum (Rud., 1819) (Tetraphyllidea, Onchobothriidae). Zeitschrift für Parasitenkunde, 47, 269–281.

    Article  Google Scholar 

  • Mokhtar-Maamouri F., Świderski Z. 1976. Ultrastructure du spermatozoïde d’un cestode Tetraphyllidea Phyllobothriidae, Echeneibothrium beauchampi Euzet, 1959. Annales de Parasitologie, 51, 673–674.

    CAS  Google Scholar 

  • Reynolds E.S. 1963. The use of lead citrate at high pH as an electronopaque stain in electron microscopy. Journal of Cell Biology, 17, 208–212.

    Article  PubMed  CAS  Google Scholar 

  • Rohde K. 1990. Phylogeny of Platyhelminthes, with special reference to parasitic groups. International Journal for Parasitology, 20, 979–1007.

    Article  PubMed  CAS  Google Scholar 

  • Schmidt G.D. 1986. CRC handbook of tapeworm identification. CRC Press, Inc., Boca Raton, Florida, 675 pp.

    Google Scholar 

  • Sène A., Bâ C.T., Marchand B. 1997. Ultrastructure of spermiogenesis and the spermatozoon of Nomimoscolex sp. (Cestoda, Proteocephalidea) intestinal parasite of Clarotes laticeps (fish, teleost) in Senegal. Journal of Submicroscopic Cytology and Pathology, 29, 115–124.

    PubMed  Google Scholar 

  • Świderski Z., Mokhtar-Maamouri F. 1980. Étude de la spermatogénèse de Bothriocephalus clavibothrium Ariola, 1899 (Cestoda: Pseudophyllidea). Archives de l’Institut Pasteur de Tunis, 57, 323–347.

    PubMed  Google Scholar 

  • Świderski Z., Mackiewicz J.S. 2002. Ultrastructure of spermatogenesis and spermatozoa of the caryophyllidean cestode Glaridacris catostomi Cooper, 1920. Acta Parasitologica, 47, 83–104.

    Google Scholar 

  • Yoneva A., Georgieva K., Mizinska Y., Georgiev B.B., Stoitsova S.R. 2006. Ultrastructure of spermiogenesis and mature spermatozoon of Skrjabinoporus merops (Cyclophyllidea, Metadilepididae). Acta Parasitologica, 51, 200–208. DOI: 10.2478/s11686-006-0031-7.

    Article  Google Scholar 

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Authors and Affiliations

  1. Laboratory “Parasites and Mediterranean Ecosystems”, Faculty of Sciences and Technics, University of Corsica, F-20250, Corte, France

    Yann Quilichini, Joséphine Foata, Cheikh Tidiane Bâ & Bernard Marchand

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  1. Yann Quilichini
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  2. Joséphine Foata
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  3. Cheikh Tidiane Bâ
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  4. Bernard Marchand
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Correspondence to Yann Quilichini.

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Quilichini, Y., Foata, J., Bâ, C.T. et al. Ultrastructure of the spermatozoon of Calliobothrium verticillatum (Cestoda, Tetraphyllidea, Oncobothriidae). Acta Parasit. 52, 219–224 (2007). https://doi.org/10.2478/s11686-007-0037-9

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