Skip to main content

Advertisement

SpringerLink
  • Log in
  1. Home
  2. Cellular & Molecular Biology Letters
  3. Article
Human adipose-derived stem cells for the treatment of intracerebral hemorrhage in rats via femoral intravenous injection
Download PDF
Your article has downloaded

Similar articles being viewed by others

Slider with three articles shown per slide. Use the Previous and Next buttons to navigate the slides or the slide controller buttons at the end to navigate through each slide.

Mannitol Augments the Effects of Systemical Stem Cell Transplantation without Increasing Cell Migration in a Stroke Animal Model

08 September 2020

Sang-Hoon Lee, Ho-Young Kang, … Dong-Hyuk Park

Immunological effects of the intraparenchymal administration of allogeneic and autologous adipose-derived mesenchymal stem cells after the acute phase of middle cerebral artery occlusion in rats

05 December 2018

Zhang Yu, Tang Wenyan, … Luan Zuo

p5 Peptide-Loaded Human Adipose-Derived Mesenchymal Stem Cells Promote Neurological Recovery After Focal Cerebral Ischemia in a Rat Model

06 May 2020

Arjun Paudyal, Flavia Semida Ghinea, … Aurel Popa-Wagner

Rebuilding hippocampus neural circuit with hADSC-derived neuron cells for treating ischemic stroke

04 April 2022

Jian Wang, Rui Hao, … Shane Gao

Early-phase administration of human amnion-derived stem cells ameliorates neurobehavioral deficits of intracerebral hemorrhage by suppressing local inflammation and apoptosis

12 February 2022

Yoji Kuramoto, Mitsugu Fujita, … Shinichi Yoshimura

Intravenous delivery of adipose tissue-derived mesenchymal stem cells improves brain repair in hyperglycemic stroke rats

17 July 2019

Mari Carmen Gómez-de Frutos, Fernando Laso-García, … on behalf of RESSTORE consortium

Human intracerebroventricular (ICV) injection of autologous, non-engineered, adipose-derived stromal vascular fraction (ADSVF) for neurodegenerative disorders: results of a 3-year phase 1 study of 113 injections in 31 patients

20 July 2019

Christopher Duma, Oleg Kopyov, … Benjamin Rapaport

Efficacy of adipose derived stem cells on functional and neurological improvement following ischemic stroke: a systematic review and meta-analysis

10 August 2020

Mahmoud Yousefifard, Jebreil Shamseddin, … Arash Sarveazad

Topical application of adipose tissue-derived mesenchymal stem cells (ADMSCs) reduced cerebral edema in experimental traumatic brain injury (TBI)—a preliminary study

04 January 2021

Hui Ma, Lian Xu Cui, … Wai Sang Poon

Download PDF
  • Research Article
  • Published: 28 April 2012

Human adipose-derived stem cells for the treatment of intracerebral hemorrhage in rats via femoral intravenous injection

  • Kuo-Liang Yang1,2,
  • Jiunn-Tat Lee3,
  • Cheng-Yoong Pang4,5,
  • Ting-Yi Lee1,
  • Shee-Ping Chen1,
  • Hock-Kean Liew4,
  • Shin-Yuan Chen6,
  • Tzu-Yung Chen7 &
  • …
  • Py-Yu Lin1 

Cellular & Molecular Biology Letters volume 17, pages 376–392 (2012)Cite this article

  • 661 Accesses

  • 30 Citations

  • 1 Altmetric

  • Metrics details

Abstract

Human adipose-derived stem cells (huADSC) were generated from fat tissue of a 65-year-old male donor. Flow cytometry and reverse transcription polymerase chain reaction (RT-PCR) analyses indicated that the huADSC express neural cell proteins (MAP2, GFAP, nestin and β-III tubulin), neurotrophic growth factors (BDNF and GDNF), and the chemotactic factor CXCR4 and its corresponding ligand CXCL12. In addition, huADSC expressed the characteristic mesenchymal stem cell (MSC) markers CD29, CD44, CD73, CD90, CD105 and HLA class I. The huADSC were employed, via a right femoral vein injection, to treat rats inflicted with experimental intracerebral hemorrhage (ICH). Behavioral measurement on the experimental animals, seven days after the huADSC therapy, showed a significant functional improvement in the rats with stem cell therapy in comparison with rats of the control group without the stem cell therapy. The injected huADSC were detectable in the brains of the huADSC treated rats as determined by histochemistry analysis, suggesting a role of the infused huADSC in facilitating functional recovery of the experimental animals with ICH induced stroke.

Download to read the full article text

Abbreviations

ADAS:

adipose-derived adult stem

ADSC:

adipose-derived stem cell

FCS:

fetal calf serum

hAdMSC:

adipose derived mesenchymal stem cell

hASC:

adipose tissue-derived stem cell

hATSC:

adipose tissue stromal cell

ICH:

intracerebral hemorrhage

MCAO:

middle cerebral artery occlusion

MSC:

mesenchymal stem cell

SVF:

stromal-vascular fraction

References

  1. Xi, G., Keep, R.F. and Hoff, J.T. Mechanisms of brain injury after intracerebral haemorrhage. Lancet Neurol. 53 (2006) 53–63.

    Article  Google Scholar 

  2. Broderick, J.P., Adams, H.P. Jr., Barsan, W., Feinberg, W., Feldmann, E., Grotta, J., Kase, C., Krieger, D., Mayberg, M., Tilley, B., Zabramski, J.M. and Zuccarello, M. Guidelines for the management of spontaneous intracerebral hemorrhage: A statement for healthcare professionals from a special writing group of the Stroke Council, American Heart Association. Stroke, 30 (1999) 905–915.

    Article  PubMed  CAS  Google Scholar 

  3. Mendelow, A.D., Gregson, B.A., Fernandes, H.M., Murray, G.D., Teasdale, G.M., Hope, D.T., Karimi, A., Shaw, M.D., Barer, D.H. and STICH investigators. Early surgery versus initial conservative treatment in patients with spontaneous supratentorial intracerebral haematomas in the International Surgical Trial in Intracerebral Haemorrhage (STICH): a randomised trial. Lancet, 365 (2005) 387–397.

    PubMed  Google Scholar 

  4. Del Bigio, M.R., Yan, H.J., Buist, R. and Peeling, J. Experimental intracerebral hemorrhage in rats. Magnetic resonance imaging and histopathological correlates. Stroke, 27 (1996) 2312–2319; discussion 2319–2320.

    Article  PubMed  Google Scholar 

  5. Gong, C., Hoff, J.T. and Keep, R.F. Acute inflammatory reaction following experimental intracerebral hemorrhage in rat. Brain Res. 871 (2000) 57–65.

    Article  PubMed  CAS  Google Scholar 

  6. Xue, M. and Del Bigio, M.R. Intracerebral injection of autologous whole blood in rats: time course of inflammation and cell death. Neurosci. Lett. 283 (2000) 230–232.

    Article  PubMed  CAS  Google Scholar 

  7. Fenstermacher, J.D., Knight, R.A., Ewing, J.R., Nagaraja, T., Nagesh, V., Yee, J.S. and Arniego, P.A. Estimating blood-brain barrier opening in a rat model of hemorrhagic transformation with Patlak plots of Gd-DTPA contrast-enhanced MRI. Acta Neurochir. Suppl. 86 (2003) 35–37.

    Article  PubMed  CAS  Google Scholar 

  8. Hoff, J.T. and Xi, G. Brain edema from intracerebral hemorrhage. Acta Neurochir. Suppl. 86 (2003) 11–15.

    Article  PubMed  CAS  Google Scholar 

  9. Kitaoka, T., Hua, Y., Xi, G., Hoff, J.T. and Keep, R.F. Delayed argatroban treatment reduces edema in a rat model of intracerebral hemorrhage. Stroke 33 (2002) 3012–3018.

    Article  PubMed  CAS  Google Scholar 

  10. MacLellan, C.L., Silasi, G., Poon, C.C., Edmundson, C.L., Buist, R., Peeling, J. and Colbourne, F. Intracerebral hemorrhage models in rat: comparing collagenase to blood infusion. J. Cereb. Blood Flow Metab. 28 (2008) 516–525.

    Article  PubMed  CAS  Google Scholar 

  11. Rincon, F. and Mayer, S.A. Novel therapies for intracerebral hemorrhage. Curr. Opin. Crit. Care, 10 (2004) 94–100.

    Article  PubMed  Google Scholar 

  12. Wu, J., Hua, Y., Keep, R.F., Nakamura, T., Hoff, J.T. and Xi, G. Iron and iron-handling proteins in the brain after intracerebral hemorrhage. Stroke, 34 (2003) 2964–2969.

    Article  PubMed  CAS  Google Scholar 

  13. Bai, X., Ma, J., Pan, Z., Song, Y.H., Freyberg, S., Yan, Y., Vykoukal, D. and Alt, E. Electrophysiological properties of human adipose tissue-derived stem cells. Am. J. Physiol. Cell Physiol. 293 (2007) C1539–C1550.

    Article  PubMed  CAS  Google Scholar 

  14. Bunnell, B.A., Flaat, M., Gagliardi, C., Patel, B. and Ripoll, C. Adiposederived stem cells: isolation, expansion and differentiation. Methods, 45 (2008) 115–120.

    Article  PubMed  CAS  Google Scholar 

  15. Gimble, J.M., Katz, A.J. and Bunnell, B.A. Adipose-derived stem cells for regenerative medicine. Circ. Res. 100 (2007) 1249–1260.

    Article  PubMed  CAS  Google Scholar 

  16. Hong, L., Peptan, I.A., Colpan, A. and Daw, J.L. Adipose tissue engineering by human adipose-derived stromal cells. Cells Tissues Organs, 183 (2006) 133–140.

    Article  PubMed  CAS  Google Scholar 

  17. Kang, S.K., Lee, D.H., Bae, Y.C., Kim, H.K., Baik, S.Y. and Jung, J.S. Improvement of neurological deficits by intracerebral transplantation of human adipose tissue-derivrd stromal cells after cerebral ischemia in rats. Exp. Neurol. 183 (2003) 355–366.

    Article  PubMed  CAS  Google Scholar 

  18. Lin, S.D., Wang, K.H. and Kao, A.P. Engineered adipose tissue of predefined shape and dimensions from human adipose-derived mesenchymal stem cells. Tissue Eng. Part A, 14 (2008) 571–581.

    Article  PubMed  CAS  Google Scholar 

  19. Witkowska-Zimny, M. and Walenko, K. Stem cells from adipose tissue. Cell. Mol. Biol. Lett. 16 (2011) 236–257.

    Article  PubMed  Google Scholar 

  20. Ohta, Y., Takenaga, M., Tokura, Y., Hamaguchi, A., Matsumoto, T., Kano, K., Mugishima, H., Okano, H. and Igarashi, R. Mature adipocyte-derived cells, dedifferentiated fat cells (DFAT), promoted functional recovery from spinal cord injury-induced motor dysfunction in rats. Cell Transplant. 17 (2008) 877–886.

    Article  PubMed  Google Scholar 

  21. Safford, K.M., Hicok, K.C., Safford, S.D., Halvorsen, Y.D., Wilkison, W.O., Gimble, J.M. and Rice, H.E. Neurogenic differentiation of murine and human adipose-derived stromal cells. Biochem. Biophy. Res. Commun. 294 (2002) 371–379.

    Article  CAS  Google Scholar 

  22. Planat-Bénard, V., Menard, C., André, M., Puceat, M., Perez, A., Garcia-Verdugo, J.M., Pénicaud, L., Casteilla, L. Spontaneous cardiomyocyte differentiation from adipose tissue stroma cells. Circ. Res. 94 (2004) 223–229.

    Article  PubMed  Google Scholar 

  23. Kim, J.M., Lee, S.T., Chu, K., Jung, K.H., Song, E.C., Kim, S.J., Sinn, D.I., Kim, J.H., Park, D.K., Kang, K.M., Hyung Hong, N., Park, H.K., Won, C.H., Kim, K.H., Kim, M., Kun Lee, S. and Roh, J.K. Systemic transplantation of human adipose stem cells attenuated cerebral inflammation and degeneration in a hemorrhagic stroke model. Brain Res. 1183 (2007) 43–50.

    Article  PubMed  CAS  Google Scholar 

  24. Yang, K.L., Chen, M.F., Liao, C.H., Pang, C.Y. and Lin, P.Y.. A simple and efficient method for generating Nurr1-positive neuronal stem cells from human wisdom teeth (tNSC) and the potential of tNSC for stroke therapy. Cytotherapy, 11 (2009) 606–617.

    Article  PubMed  CAS  Google Scholar 

  25. Rosenberg, G.A., Mun-Bryce, S., Wesley, M. and Kornfeld, M. Collagenase-induced intracerebral hemorrhage in rats. Stroke, 21 (1990) 801–807.

    Article  PubMed  CAS  Google Scholar 

  26. MacLellan, C.L., Silasi, G., Poon, C.C., Edmundson, C.L., Buist, R., Peeling, J. and Colbourne, F. Intracerebral hemorrhage models in rat: comparing collagenase to blood infusion. J. Cereb. Blood Flow Metab. 28 (2008) 516–525.

    Article  PubMed  CAS  Google Scholar 

  27. Chen, J., Li, Y., Wang, L., Zhang, Z., Lu, D., Lu, M. and Chopp, M. Therapeutic benefit of intravenous administration of bone marrow stromal cells after cerebral ischemia in rats. Stroke, 32 (2001) 1005–1011.

    Article  PubMed  CAS  Google Scholar 

  28. Witkowska-Zimny, M. and Wrobel, E. Perinatal sources of mesenchymal stem cells: Wharton’s jelly, amnion and chorion. Cell. Mol. Biol. Lett. 16 (2011) 493–514.

    Article  PubMed  Google Scholar 

  29. Klassen, H., Schwartz, M.R., Bailey, A.H. and Young, M.J. Surface markers expressed by multipotent human and mouse neural progenitor cells include tetraspanins and non-protein epitopes. Neurosci. Lett. 312 (2001) 180–182.

    Article  PubMed  CAS  Google Scholar 

  30. Zuk, P.A., Zhu, M., Ashjian, P., De Ugarte, D.A., Huang, J.I., Mizuno, H., Alfonso, Z.C., Fraser, J.K., Benhaim, P. and Hedrick, M.H. Human adipose tissue is a source of multipotent stem cells. Mol. Biol. Cell. 13 (2002) 4279–4295.

    Article  PubMed  CAS  Google Scholar 

  31. Chen, L., He, D.M. and Zhang, Y. The differentiation of human placentaderived mesenchymal stem cells into dopaminergic cells in vitro. Cell. Mol. Biol. Lett. 14 (2009) 528–536.

    Article  PubMed  CAS  Google Scholar 

  32. Mahmood, A., Lu, D., Wang, L. and Chopp, M. Intracerebral transplantation of marrow stromal cells cultured with neurotrophic factors promotes functional recovery in adult rats subjected to traumatic brain injury. J. Neurotrauma, 19 (2002) 1609–1617.

    Article  PubMed  Google Scholar 

  33. Nakagami, H., Maeda, K., Morishita, R., Iguchi, S., Nishikawa, T., Takami, Y., Kikuchi, Y., Saito, Y., Tamai, K., Ogihara, T. and Kaneda, Y. Novel autologous cell therapy in ischemic limb disease through growth factor secretion by cultured adipose tissue-derived stromal cells. Arterioscler. Thromb. Vasc. Biol. 25 (2005) 2542–2547.

    Article  PubMed  CAS  Google Scholar 

  34. Tate, C.C., Fonck, C., McGrogan, M. and Case, C.C. Human mesenchymal stromal cells and their derivative, SB623 cells, rescue neural cells via trophic support following in vitro ischemia. Cell Transplant. 19 (2010) 973–984.

    Article  PubMed  Google Scholar 

  35. Harting, M.T., Jimenez, F., Xue, H., Fischer, U.M., Baumgartner, J., Dash, P.K. and Cox, C.S. Intravenous mesenchymal stem cell therapy for traumatic brain injury. J. Neurosurg. 110 (2009) 1189–1197.

    Article  PubMed  CAS  Google Scholar 

  36. Schrepfer, S., Deuse, T., Reichenspurner, H., Fischbein, M.P., Robbins, R.C., Pelletier, M.P. Stem cell transplantation: the lung barrier. Transplant. Proc. 39 (2007) 573–576.

    Article  PubMed  CAS  Google Scholar 

  37. Mahmood, A., Lu, D. and Chopp, M. Intravenous administration of marrow stromal cells (MSCs) increases the expression of growth factors in rat brain after traumatic brain injury. J. Neurotrauma, 21 (2004) 33–39.

    Article  PubMed  Google Scholar 

  38. Lin, G., Yang, R., Banie, L., Wang, G., Ning, H., Li, L.C., Lue, T.F., Lin, C.S. Effects of transplantation of adipose tissue-derived stem cells on prostate tumor. Prostate, 70 (2010) 1066–1073.

    Article  PubMed  Google Scholar 

  39. Chung, S., Sonntag, K.C., Andersson, T., Bjorklund, L.M., Park, J.J., Kim, D.W., Kang, U.J., Isacson, O. and Kim, K.S. Genetic engineering of mouse embryonic stem cells by Nurr1 enhances differentiation and maturation into dopaminergic neurons. Eur. J. Neurosci. 16 (2002) 1829–1838.

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Buddhist Tzu Chi Stem Cells Center, Buddhist Tzu Chi Medical Center, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan

    Kuo-Liang Yang, Ting-Yi Lee, Shee-Ping Chen & Py-Yu Lin

  2. Institute of Medical Biotechnology, Buddhist Tzu Chi University, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan

    Kuo-Liang Yang

  3. Division of Plastic Surgery, Department of Surgery, Buddhist Tzu Chi Medical Center and Buddhist Tzu Chi University, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan

    Jiunn-Tat Lee

  4. Department of Research, Buddhist Tzu Chi Medical Center, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan

    Cheng-Yoong Pang & Hock-Kean Liew

  5. Graduate Institute of Clinical Medicine, Buddhist Tzu Chi University, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan

    Cheng-Yoong Pang

  6. Division of Functional Neuroscience, Department of Neurosurgery, Neuro-Medical Scientific Center, Buddhist Tzu Chi Medical Center, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan

    Shin-Yuan Chen

  7. Department of Neurosurgery, Buddhist Tzu Chi Medical Center Taichung Branch, Buddhist Compassion Relief Tzu Chi Foundation, Taichung, Taiwan

    Tzu-Yung Chen

Authors
  1. Kuo-Liang Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jiunn-Tat Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Cheng-Yoong Pang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ting-Yi Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shee-Ping Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hock-Kean Liew
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Shin-Yuan Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Tzu-Yung Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Py-Yu Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kuo-Liang Yang.

Electronic supplementary material

Supplementary material, approximately 1 MB.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Yang, KL., Lee, JT., Pang, CY. et al. Human adipose-derived stem cells for the treatment of intracerebral hemorrhage in rats via femoral intravenous injection. Cell Mol Biol Lett 17, 376–392 (2012). https://doi.org/10.2478/s11658-012-0016-5

Download citation

  • Received: 27 September 2011

  • Accepted: 18 April 2012

  • Published: 28 April 2012

  • Issue Date: September 2012

  • DOI: https://doi.org/10.2478/s11658-012-0016-5

Share this article

Anyone you share the following link with will be able to read this content:

Sorry, a shareable link is not currently available for this article.

Provided by the Springer Nature SharedIt content-sharing initiative

Key words

  • Adipose-derived stem cells
  • Adult stem cells
  • Adipose tissue
  • Intracerebral hemorrhage
  • Intravenous stem cell injection
  • Neural stem cells
  • Regenerative medicine
  • Stem cell therapy
  • Stroke
  • Stromal cells
Download PDF

Advertisement

Over 10 million scientific documents at your fingertips

Switch Edition
  • Academic Edition
  • Corporate Edition
  • Home
  • Impressum
  • Legal information
  • Privacy statement
  • California Privacy Statement
  • How we use cookies
  • Manage cookies/Do not sell my data
  • Accessibility
  • FAQ
  • Contact us
  • Affiliate program

Not logged in - 34.232.63.94

Not affiliated

Springer Nature

© 2023 Springer Nature Switzerland AG. Part of Springer Nature.