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Central European Journal of Medicine

, Volume 9, Issue 2, pp 193–199 | Cite as

Scutellarein ameliorates tongue cancer cells via mitochondria

Research Article
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Abstract

To investigate the mechanism in Scutellarein-induced apoptosis of SAS human tongue cancer cells, inhibitory effects of Scutellarein on SAS cells were detected by MTT assay. Cell apoptosis was analyzed by flow cytometry. Ultrastructural changes of SAS cells were observed by transmission electron microscopy (TEM). Mitochondrial transmembrane potential (ΔΨm) were analyzed by JC-1 [5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide]. Western blotting was used to examine the expression level of Bcl-2, Bax and caspase-3 in SAS cells treated with Scutellarein. Scutellarein inhibited the proliferation of SAS cells in a time and dose-dependent manner and increased the percent of apoptotic cells. The mitochondrial cristae were swollen and had vacuolar degeneration. ΔΨm decreased when the concentration of scutellarein increased. Scutellarein effectively up-regulated the expression of mitochondrial Bax and caspase-3 and down-regulated the expression of Bcl-2. Scutellarein induces apoptosis of SAS human tongue cancer cells via activating mitochondrial signaling pathway.

Keywords

Scutellarein Mitochondria Apoptosis SAS Tongue cancer cell 

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Copyright information

© Versita Warsaw and Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Department of Oral Anatomy & Physiology, Stomatological CollegeHarbin Medical UniversityHarbinChina
  2. 2.Department of Anatomy, Basic Medical Science CollegeHarbin Medical UniversityHarbinChina
  3. 3.Department of Oral & Maxillofacial Surgery, Stomatological CollegeHarbin Medical UniversityHarbinChina

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