Central European Journal of Medicine

, Volume 7, Issue 5, pp 599–603 | Cite as

Macrophages, TGF-β1 expression and iron deposition in development of NASH

  • Julian Ananiev
  • Mariana Penkova
  • Georgi Tchernev
  • Maya Gulubova
Research Article


A wide range of molecular markers and different types of cells in liver are possible factors for progression of non-alcoholic fatty liver disease (NAFLD), non-alcoholic steatohepatitis (NASH) development of liver fibrosis. We investigated biopsies from 57 patients with NASH. The material was obtained from livers and was proceed immunohistochemistry antibodies against CD68 and TGF-beta 1. In addition, biopsies were evaluated for iron content. Macrophages/-positive/could be found in all 57 cases. The number of macrophages in the sinusoids correlated with the degree of portal fibrosis:64.% of the patients with mild or intensive fibrosis had high infiltration with CD68-positive cells, while 100% of the patients without fibrosis hadlow infiltration (χ2=8.56; p=0.003). In specimens we, 69.% of patients with different degree of fibrosis expressed TGF-β1 in their portal tracts, and 100% of patients without fibrosis did demonstrate expression of the protein (χ2=23.7; p<0.001). Hepatic iron was found in 100% (9) of patients with intensive fibrosis vs. 10.3% of the patients mild fibrosis (χ2=23.4; p<0.001). Our results suggest that the macrophages and macrophage-derived TGF-beta1 are the major factors responsible for development of fibrosis and progression of chronic liver disease.


NASH CD68 TGF-beta1 Iron 


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Copyright information

© Versita Warsaw and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Julian Ananiev
    • 1
  • Mariana Penkova
    • 2
  • Georgi Tchernev
    • 3
  • Maya Gulubova
    • 1
  1. 1.Department of General and Clinical Pathology and Forensic Medicine, Medical FacultyTrakia UniversityStara ZagoraBulgaria
  2. 2.Department of Internal medicine, Medical FacultyTrakia UniversityStara ZagoraBulgaria
  3. 3.Department of Dermatology and Venerology, Medical FacultyTrakia UniversityStara ZagoraBulgaria

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