Skip to main content
Log in

Mechanical treatment and autoclaving of middle ear ossicles from cholesteatomatous ears

  • Research Article
  • Published:
Central European Journal of Medicine

Abstract

Aim of the study: The authors present a prospective histomorphological study of middle ear ossicles, harvested from cholesteatomous ears, to evaluate for presence of residual cholesteatoma after mechanical treatment and short-time autoclaving.

Materials and methods

Sixty ossicles were used as specimens in this study. The ossicles were grouped as follows: Group A — ossicles stripped and burred using a fine diamond burr under microscopic control. Group B — ossicles autoclaved for 4 min at 134°C after mechanical burring under microscopic control.

Results

In group A, 30 ossicles were histologicaly examined after mechanical treatment. The residual disease was found in one badly eroded ossicle. In group B, 30 ossicles were histologicaly examined after mechanical treatment and autoclaving. That treatment eliminated cholesteatoma in all cases. The difference of results in these two treatment methods was not statistically significant.

Conclusion

Authors recommend using mechanical cleaning in cases of mildly eroded ossicles. In cases of badly eroded ossicles authors recommend to either apply a combination of mechanical and autoclaving treatment. The study proved that 4 minutes of autoclaving at 134°C after mechanical cleaning provides safe bone autografts.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Hall A, Rytzner C. Stapedectomy and autotransplantation of ossicles. Acta Otolaryngol. 1957 Apr;47(4):318–324

    Article  PubMed  CAS  Google Scholar 

  2. Dornhoffer JL, Colvin GB, North P. Evidence of residual disease in ossicles of patients undergoing cholesteatoma removal. Acta Otolaryngol. 1999 Jan;119(1):89–92

    Article  PubMed  CAS  Google Scholar 

  3. el Seifi A, Fouad B. Autograft ossiculoplasty in cholesteatoma. ORL J Otorhinolaryngol Relat Spec. 1992;54(6):324–327

    Article  PubMed  Google Scholar 

  4. Miman MC, Aydin NE, Oncel S, Ozturan O, Erdem T. Autoclaving the ossicles provides safe autografts in cholesteatoma. Auris Nasus Larynx. 2002 Apr;29(2):133–139

    Article  PubMed  Google Scholar 

  5. Navratil J, Kotrle M. Morphological changes of the auditory ossicles in chronic otitis. Cs. Otolaryng. 1964, 13(5):305–308

    CAS  Google Scholar 

  6. Ng SK, Yip WW, Suen M, Abdullah VJ, van Hasselt CA. Autograft ossiculoplasty in cholesteatoma surgery: is it feasible? Laryngoscope. 2003 May;113(5):843–847

    Article  PubMed  Google Scholar 

  7. Quaranta A, Bartoli R, Lozupone E, Resta L, Iurato S. Cholesteatoma in children: histopathologic findings in middle ear ossicles. ORL J Otorhinolaryngol Relat Spec. 1995 Sep-Oct;57(5):296–298

    Article  PubMed  CAS  Google Scholar 

  8. Rupa V, Krishnaswami H, Job A. Autograft ossicle selection in cholesteatomatous ear disease: histopathological considerations. J Laryngol Otol. 1997 Sep;111(9):807–809

    Article  PubMed  CAS  Google Scholar 

  9. Sadé J. Epithelial invasion of intraossicular spaces. J Laryngol Otol. 1972 Jan;86(1):15–21

    Article  PubMed  Google Scholar 

  10. Subotić R, Femenić B. Histological changes of incus with cholesteatoma in the attic. Acta Otolaryngol. 1991;111(2):358–361

    Article  PubMed  Google Scholar 

  11. Khan I, Jan AM. Middle ear reconstruction: a review of 150 cases. J Laryngol Otol. 2002 Jun;116(6):435–439

    Article  PubMed  Google Scholar 

  12. Yung M. Long-term results in ossiculoplasty: reason for surgical failure. Otol Neurotol. 2006 Jan;27(1):20–26

    Article  PubMed  Google Scholar 

  13. Yung M, Vowler SL. Long-term results in ossiculoplasty: an analysis of prognostic factors. Otol Neurotol. 2006 Sep;27(6):874–881

    Article  PubMed  Google Scholar 

  14. O’Reilly RC, Cass SP. Hirsch BE, Kameren DB, Bernat RA, Poznanovic SP. Ossiculoplasty using incus interposition: hearing results and analysis of the middle ear index. Otol Neurotol. 2005 Sep;26:853–858

    Article  PubMed  Google Scholar 

  15. Bahmad F, Merchant SN. Histopathology of ossicular grafts and implants in chronic otitis media. Ann Otol Rhinol Laryngol. 2007 Mar;116(3):181–191

    PubMed  Google Scholar 

  16. Mills RP, Cree IA. Histological fate of cortical bone autografts in middle ears. Clin Otolaryngol Allied Sci. 1995 Aug;20:365–367

    Article  PubMed  CAS  Google Scholar 

  17. Merchant SN, Nadol JB Jr. Histopathology of ossicular implants. Otolaryngol Clin North Am. 1994 Mar;27:813–833

    PubMed  CAS  Google Scholar 

  18. Vartiainen E, Karjalainen S. Autologous ossicle and cortical bone in ossicular reconstruction. Clin Otolaryngol Allied Sci. 1985 Dec;10(6):307–310

    Article  PubMed  CAS  Google Scholar 

  19. Actis AB, Obwegeser JA, Rupérez C. Influence of different sterilization procedures and partial demineralization of screws made of bone on thein mechanical properties. J Biomater Appl. 2004 Jan;18(3):193–207

    Article  PubMed  CAS  Google Scholar 

  20. Inokuchi T, Ninomiya H, Hironaka R, Yoshida S, Araki M, Sano K. Studies on heat treatment for immediate reimplantation of resected bone. J Craniomaxillofac Surg. 1991 Jan;19(1):31–39

    Article  PubMed  CAS  Google Scholar 

  21. Köhler P, Kreicbergs A, Strömberg L. Physical properties of autoclaved bone Torsion test of rabbit diaphyseal bone. Acta Orthop Scand. 1986 Apr;57(2):141–145

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Lukas Skoloudik.

About this article

Cite this article

Skoloudik, L., Vokurka, J. & Simakova, E. Mechanical treatment and autoclaving of middle ear ossicles from cholesteatomatous ears. cent.eur.j.med 7, 194–197 (2012). https://doi.org/10.2478/s11536-011-0140-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2478/s11536-011-0140-z

Keywords

Navigation