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Effect of Giardia duodenalis in protein malnourished and renourished mice

  • Research Article
  • Published:
Central European Journal of Medicine

Abstract

The present study was designed to delineate the effect of Giardia duodenalis in malnourished and renourished BALB/c mice. Control and renourished mice were fed with a standard pellet diet while malnourished mice were fed with a low protein (4.3 %) diet both before and after being challenged orally with actively growing G. duodenalis trophozoites. It was observed that malnourished mice had a greater severity and longer duration of Giardia infection compared with renourished mice. These malnourished mice also had less body mass but higher cyst and trophozoite counts. Malnourished mice infected with Giardia had significantly decreased level of total serum proteins, albumin, globulins, hemoglobin, leukocyte, and differential leukocyte counts compared with renourished mice. From the data it is concluded that protein malnutrition profoundly affects the anthropometric and physiological parameters of the body indicating greater susceptibility and severity of the disease.

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References

  1. McLaren S., Disease related malnutrition in hospital and the community, Readers response, 2009, http://www.nursingtimes.net

  2. Maitland K., Berkley J.A., Shebbe M., Peshu N., English M., Children with severe malnutrition: can those at highest risk of death be identified with the WHO protocol? PLoS Med, 2006, 3, 2431–2439

    Article  Google Scholar 

  3. Rice A.L., Sacco L., Hyder A., Black R.E., Malnutrition as an underlying cause childhood deaths associated with infectious diseases in developing countries, Bulletin W.H.O., 2000, 78, 1207–1221

    CAS  Google Scholar 

  4. Girman T., Molgaard C., Michaelsen K.F., Appropriate management of severe malnutrition greatly contributes of reduction of child mortality rate, J. Pedia Gastroenterol. and Nutr., 2006, 43, 436–438

    Article  Google Scholar 

  5. Solomons N.W, Malnutrition and infection: an update, Brit. J. of Nutr, 98, 2007, S5–S10.

    CAS  Google Scholar 

  6. Prentice A.M., Gershwin M.E., Schaible U.E., Kausch G.T., Victoria C.G., Gordon. J.I., New Challenges in studying nutrition — diseases interactions in the developing world, J. Clin. Invest., 2008, 118, 1322–1329

    Article  PubMed  CAS  Google Scholar 

  7. Blackburn F.L., Pasteur’s quardrant and malnutrition, Nature, 2001, 409, 397–401.

    Article  PubMed  CAS  Google Scholar 

  8. Katelaris P.H., Naeem A., Fathing M.J.G., Attachment of Giardia lamblia trophozoites to a cultures human intestinal cell line, Gut, 1995, 37, 512–518

    Article  PubMed  CAS  Google Scholar 

  9. Eckmann L., Mucosal defences against Giardia, Parasite Immunol., 2003, 25, 259–270.

    Article  PubMed  CAS  Google Scholar 

  10. Gardner T.B., Hill D.R., Treatment of giardiasis, Clin. Mircobiol. Rev. 2001, 14, 114–128

    Article  CAS  Google Scholar 

  11. Scott K.G.E., Xu L.C.H., Buret A.G., Role of CD8+ and CD4+ T lymphocytes in jejunal mucosal injury during murine giardiasis, Infect. Immun., 2004, 72, 3536–3542

    Article  PubMed  CAS  Google Scholar 

  12. Diamond L.S., Techniques of anexic culture of E. histolytica Schaudinn, 1903 and E. histolytica like amoebae, J. Parasitol. 1963, 64, 1047–1056

    Google Scholar 

  13. Villena J., Racedo S., Aguero G, Alvarez S., Yoghurt accelerates and recovery of defense mechanisms against Streptococcus pneumoniae in protein-malnourished mice, Brit. J. Nutr., 2006, 95, 591–602

    Article  PubMed  CAS  Google Scholar 

  14. Tiwari R.P., Hoondal G.S., Tewari R., Laboratory Techniques in Microbiology and Biotechnology, Abhishek Publication., 2001

  15. Cano P.G., Ageuro G., Pergigon G., Adjuvant effects of Lactobacillus casei added to a renutrition diet in a malnourished mouse model, Biocell, 2002, 26, 35–48

    Google Scholar 

  16. Dock D.B., Nascimento J.E., Lactorraca M.Q., Probiotics enhance the recovery of gut atrophy in experimental malnutrition, Biocell., 2004, 28, 143–150

    PubMed  Google Scholar 

  17. Shukla G., Devi P., Sehgal R., Effect of Lactobacillus casei as a probiotic on modulation of giardiasis, Dig. Dis. Sci., 2008, 53, 2671–2679

    Article  PubMed  Google Scholar 

  18. Udall J.N., Bhutta Z.A., Firmansyah A., Gyoyens P., Lantze M.J., Fifschitz C,. Malnutrition and Diarrhea: working group report of the first world congress and pediatric gastroenterology, hepotology and nutrition, J. Pedia. Gastroenterol. and Nutr., 2002. 35, 173–179

    Article  Google Scholar 

  19. Muller O., Krawinkel M., Malnutrition and healthy in developing countries, Can.. Med. Assoc. J., 2005, 173, 279–286

    Article  Google Scholar 

  20. Hughes S., Kelly P, Interaction of malnutrition and immune impairment, with specific reference to immunity against parasites, Parasite Immunol., 2006, 28, 577–588

    PubMed  CAS  Google Scholar 

  21. Louria D.B., Under nutrition can affect the invading micro-organism, Clin. Inf. Dis., 2007, 45, 470–474.

    Article  Google Scholar 

  22. Lopez-Pedrosa J.M., Torres M.I., Fernandez M.I., Rios A., Gil A., Severe malnutrition alters lipid composition and fatty acid profile of small intestine in newborn piglets,. J. Nutr., 1997, 128, 224–233

    Google Scholar 

  23. Herems C., Azevedo J.F., Avaujo E.J.A., Sant — Ana D.M.G., Intestinal ascending colon morphmetrics in rats submitted to severe protein malnutrition, Intr. J. Morphol., 2008, 26, 5–11

    Google Scholar 

  24. Upadhay P., Ganguly N.K., Mahajan R.C., Walia B.N.S., Intestinal uptake of nutrients in normal and malnourished animals infected with Gairdia lamblia, Digestion., 1985, 32, 243–248

    Article  Google Scholar 

  25. Delgado M.G.H., Gilman R.H., Bern C., Cabrera. L., Sterling C.R, Black R.E, Checkley W, Lack of an adverse effect of Giardia duodenalis infection on health of Peruvian children, Am. J. Epi., 2008, 168: 647–655.

    Article  Google Scholar 

  26. Awasthi S., Pande V.K., Prevalence of malnutrition and intestinal parasites in preschool slum children in Lucknow, Ind. Pediatr., 1997, 34, 599–604

    CAS  Google Scholar 

  27. Matos S.M.A., Assis A.M.O., Prado M.B., Strina A., Santos L.A., Jesus S.R., Barreto M.L., Giardia duodenalis infections and antropometric status in preschooler in Salvador, Bahia state, Brazil, Cad. Saude. Publica., 2008, 24, 1527–1535.

    Google Scholar 

  28. Leitch G.J., Udezulu I.A., He Q., Visvesvara G.S., Effect of protein malnutrition on experimental giardiasis in Mangolian Gerbil, Scand. J. Gastroenterol., 1993, 28, 885–893.

    Article  PubMed  CAS  Google Scholar 

  29. Khanna R., Vinayak V.K., Mehta F.S., Kumkum R.K., Cain C.K., Giardia lamblia infection in immunosuppressed animals causes severe alternations to brush border membrane enzymes, Dig. Dis. Sci., 1988, 33, 1147–1152

    Article  PubMed  CAS  Google Scholar 

  30. Nascimento D.B.D., Junqueira K., Nascimento J.E.A., Rapid restoration of colonic goblet cells induced by a hydrolyzed diet containing probiotic in experimental malnourished, Acta. Circurgica. Brasileria., 2007, 22, 72–76

    Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, Panjab University, Chandigarh, 160014, India

    Geeta Shukla & Ramandeep Kaur Sidhu

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  1. Geeta Shukla
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  2. Ramandeep Kaur Sidhu
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Correspondence to Geeta Shukla.

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Shukla, G., Sidhu, R.K. Effect of Giardia duodenalis in protein malnourished and renourished mice. cent.eur.j.med 6, 762–769 (2011). https://doi.org/10.2478/s11536-011-0078-1

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  • DOI: https://doi.org/10.2478/s11536-011-0078-1

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