Central European Journal of Medicine

, Volume 6, Issue 2, pp 137–147 | Cite as

RNA interference and its therapeutic potential

  • Anubrata Ghosal
  • Ahmad Humayan Kabir
  • Abul Mandal
Review Article


RNA interference is a technique that has become popular in the past few years. This is a biological method to detect the activity of a specific gene within a cell. RNAi is the introduction of homologous double stranded RNA to specifically target a gene’s product resulting in null or hypomorphic phenotypes. This technique involves the degradation of specific mRNA by using small interfering RNA. Both microRNA (miRNA) and small interfering RNA (siRNA) are directly related to RNA interference. RNAi mechanism is being explored as a new technique for suppressing gene expression. It is an important issue in the treatment of various diseases. This review considers different aspects of RNAi technique including its history of discovery, molecular mechanism, gene expression study, advantages of this technique against previously used techniques, barrier associated with this technique, and its therapeutic application.


Cancer MicroRNA Interfering RNA Disease 


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Copyright information

© © Versita Warsaw and Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Anubrata Ghosal
    • 1
  • Ahmad Humayan Kabir
    • 2
  • Abul Mandal
    • 3
  1. 1.Institute of Cellular and Molecular MedicineUniversity of CopenhagenCopenhagenDenmark
  2. 2.School of Biological SciencesFlinders UniversityFlindersAustralia
  3. 3.System Biology Research Center, School of Life SciencesUniversity of SkövdeSkövdeSweden

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