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Central European Journal of Medicine

, Volume 6, Issue 2, pp 181–184 | Cite as

The effect of a leukodepletion model on the activation stage of platelets

  • Miodrag Vucic
  • Ivan Tijanic
  • Nenad Govedarevic
  • Lana Macukanovic
  • Zoran Pavlovic
Research Article
  • 48 Downloads

Abstract

The preparation of thrombocyte concentrates with filtration before storage (in-line) makes it possible to avoid the presence of mononuclear cells in the concentrate and proinflammatory cytokines. Therefore, this filtration may result with decreased activation of trombocyte receptors in vitro, which may improve therapeutic efficiancy. Methods. We compared two groups, each with 30 therapeutic doses of concentrated thrombocytes. We prepared the first group using the classic model from the buffy coat and the other with concentrated thrombocyte samples filtrated during sampling, so-called in-line, with the WBC filter Imuflex (Terumo). Mononuclear cells (MNC), thrombocyte, and erythrocyte counts in the units of concentrated thrombocytes were obtained on an automatic cell counter, and we used flow cytometry to measure the expression of surface thrombocyte receptors. The results demonstrated that the trombocytes prepared with pre-storage filtration contained a very low level of mononuclear cells and markedly reduced trombocyte receptors. Conclusion. The number of MNC and expression of surface thrombocyte receptors were markedly lower in the concentrated thrombocyte units prepared with in-line filtration. The thrombocytes prepared in this way contain fewer mononuclear cells, are of higher quality, are more functional, and may produce a better therapeutic effect in vivo.

Keywords

Thrombocytes Thrombocyte receptors In-line WBC filtration 

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Copyright information

© © Versita Warsaw and Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Miodrag Vucic
    • 1
  • Ivan Tijanic
    • 1
  • Nenad Govedarevic
    • 1
  • Lana Macukanovic
    • 1
  • Zoran Pavlovic
    • 1
  1. 1.Clinic of Hematology, Clinical Centre NisUniversity in NisNisSerbia

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