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The effect of vitamin C on amiodarone-induced toxicity in rat thymocytes

Central European Journal of Medicine volume 6, pages 58–63 (2011)Cite this article

Abstract

Although, the antiarrhythmic effect of amiodarone (AMD) is well characterized, the mechanism of its toxicity on extracardiac tissues is still poorly understood. Several antioxidants have been shown to prevent AMD-induced toxicity by antioxidant and/or non-antioxidant mechanisms. In the current study, we evaluated the possible protective effect, in vitro, of vitamin C on AMD-induced toxicity in rat thymocytes. Rat thymocytes were cultured with increasing AMD concentrations (1–20 μM) with or without vitamin C (1000 μg/ml), for 24 hours. Cells treatment with AMD resulted in a concentration-dependent increase of hypodiploid cells and a significant decrease in cellular glutathione content. Vitamin C combined with AMD significantly decreased the proportion of hypodiploid cells and markedly increased the cellular glutathione content, compared with AMD treatment alone. These results suggest that treatment with vitamin C may prevent AMD-induced toxicity in rat thymocytes by restoring cellular glutathione content.

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Authors and Affiliations

  1. Institute of Physiology, Medical Faculty University in Nis, 18000, Nis, Serbia

    S. Cekic & V. Pavlovic

  2. Institute of Biochemistry, Medical Faculty University in Nis, 18000, Nis, Serbia

    D. Pavlovic

  3. Medical Faculty University in Nis, 18000, Nis, Serbia

    M. Sarac

  4. Clinic of Pediatrics, Medical Faculty University in Nis, 18000, Nis, Serbia

    B. Kamenov

  5. Institute for Prevention, Treatment and Rehabilitation of Rheumatic and Cardiovascular Diseases, Medical Faculty University in Nis, 18000, Nis, Serbia

    A. Dimic

Authors
  1. S. Cekic
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  2. D. Pavlovic
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  3. M. Sarac
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  4. B. Kamenov
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  5. A. Dimic
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  6. V. Pavlovic
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Correspondence to V. Pavlovic.

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Cekic, S., Pavlovic, D., Sarac, M. et al. The effect of vitamin C on amiodarone-induced toxicity in rat thymocytes. cent.eur.j.med 6, 58–63 (2011). https://doi.org/10.2478/s11536-010-0050-5

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  • DOI: https://doi.org/10.2478/s11536-010-0050-5

Keywords

  • Amiodarone
  • Vitamin
  • Thymocytes
  • Apoptosis
  • Glutathione