Apoptosis quantification at the respiratory epithelium level in asthma
- 44 Downloads
The „changes” occurring in the expression of the factors involved in the apoptotic chain at the level of the respiratory epithelium in asthma is still an unsolved issue. At this level an important role is played by the mitochondria and the factors that influence the membrane permeability, especially the Bcl-2 super family. The purpose of this study is to evaluate both the changes in the expression of the Bcl-2 and of the Bax proapoptotic factors at the respiratory epithelium level in 21 patients with bronchial asthma of different degrees of severity of disease (aaccording to GINA — Global Initiative For Asthma). To accomplish this, fragments of the bronchial mucosa were obtained through fiberbronchoscopy, being afterwords hystologically prepare in view of the immunomarking with anti Bcl-2 and anti-Bax antibodies. Microscopic examination revealed an important decrease in the level of proapoptotic factor Bcl-2 in patients with persistent severe forms of the disease and a significant decrease in the expression of the proapototic Bax factor at the respiratory epithelium level even in the early stages of the disease. Knowing all the factors involved in apoptosis at the respiratory epithelium level in bronchial asthma, as well as of their expression changes will be at the core of new therapeutical approaches to of this disease.
KeywordsBronchial asthma Apoptosis Bax Bcl-2 Respiratory epithelium
Unable to display preview. Download preview PDF.
- Dorschei D.R, Wojcik K.R., Sun S., Marroquin B., White S.R., Apoptosis of airway epithelial cells induced by corticosteroids, Am. J. Respir. Crit. Care Med., 2001, 164:1939–47Google Scholar
- Cohen L., Tarsi J., Ramkumar T., Horiuchi T.K., Cochran R., DeMartino S., et al., and the NHLBI Severe Asthma Research Program (SSARP), Epithelial cell proliferation contributes to airway remodeling in severe asthma, Am. J. Respir. Crit. Care Med., 2007, 15;176(2):138–45. Epub 2007 Apr 26CrossRefGoogle Scholar
- White S.R., Dorscheid D.R., Corticosteroid-Induced Apoptosis of Airway Epithelium — A Potential Mechanism for Chronic Airway Epithelial Damage in Asthma, Chest, 2002, 22:2278S–2284SGoogle Scholar
- Fahy J.V., Goblet cell and mucin gene abnormalities in sthma, Chest, 2002, 22(66 Suppl):3320S–3326SGoogle Scholar
- Xiang J., Rir-Sim-Ah J., Tesfaigzi Y., IL-9 and IL-13 induce mucous cell metaplasia that is reduced by IFN-gamma in a Bax-mediated pathway, Am. J.Respir. Cell. Mol. Biol., 2008, 38(33):3310–7. Epub 2007 ep 7Google Scholar