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Central European Journal of Medicine

, Volume 5, Issue 4, pp 470–474 | Cite as

Faecal shedding of Arcobacter species following experimental infection in rats: Public health implications

Research Article
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Abstract

Arcobacter spp. are emerging food borne pathogens associated with prolonged diarrhea and occasional systemic infections such as bactereamia and peritonitis in humans. Information on faecal shedding patterns to assess the potential role they play within the intestine however, is lacking. This study was designed to investigate faecal shedding of local isolates of Arcobacter spp. Using real time PCR for confirmation, A. cryaerophilus and A. butzleri were isolated from the stool of healthy chickens. Pathogenicity of the organisms was tested by administering a single oral challenge of 102–109 cfu/ml to 45 healthy adult male albino rats divided equally among 5 groups. Uninfected rats were used as the control group. A. cryaerophilus and A. butzleri produced infection in 100% of the animals. Experimental infection was dose dependent and caused diarrheal illness and faecal shedding was noted up to 5 weeks post infection. The present study demonstrates that rats can act as a reservoir and potential source of Arcobacter infection in humans and animals exposed to this pathogen.

Keywords

Arcobacter Feacal shedding Public health 

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References

  1. [1]
    Philips CA (2001). Arcobacter species in food: isolation, identification and control. Trends foods Sci. Technol. 12:263–275CrossRefGoogle Scholar
  2. [2]
    Moreno Y, Botella S, Alonso JL, Ferrús, MA, Hernández M and Hernández J (2003). Specific Detection of Arcobacter and Campylobacter strains in water and sewage by PCR and fluorescent in situ hybridization, Appl. Environ. Microbiol. 69:1181–1186CrossRefPubMedGoogle Scholar
  3. [3]
    Houf K, Devriese LA, Haesebrouck F, Vandenberg O, Butzler JP, Van Hoof J and Vandamme P (2004). Antimicrobial susceptibility patterns of Arcobacter Arcobacter and Arcobacter cryaerophilus strains isolated from humans and broilers, Microb. Drug Resist.10:243–247PubMedGoogle Scholar
  4. [4]
    Lastovica AJ and Skirrow MB (2000). Clinical significance of Campylobacter and related species other than Campylobacter jejuni and C. coli. In: I. Nachamkin and M.J. Blaser, Editors, Campylobacter (2nd ed.), American Society for Microbiology, Washington, DC (2000), pp. 89–120Google Scholar
  5. [5]
    Rice EW, Rodgers MR, Wesley IV, Johnson CH and Tanner SA (1999). Isolation of Arcobacter Arcobacter from ground water, Lett. Appl. Microbiol. 28:31–35CrossRefPubMedGoogle Scholar
  6. [6]
    Kiehlbauch JA, Brenner DJ, Nicholson MA, Baker CN, Pathon CM, Steigerwalt AG et al., (1991b) C. Arcobacter species nov. isolated from humans and animals with diarrhoea illness. J Clin. Microbiol.; 29:376–85PubMedGoogle Scholar
  7. [7]
    Ongor H, Cetinkaya B, Acik MN and Atabay HI (2004). Investigation of Arcobacters in meat and faecal samples of clinically healthy cattle in Turkey, Lett. Appl. Microbiol. 38: 339–344CrossRefPubMedGoogle Scholar
  8. [8]
    Van Driessche E, Houf K, Van Hoof J, De Zutter L and Vandamme P (2003). Isolation of Arcobacter species from animal feces, FEMS Microbiol. Lett. 229:243–248CrossRefPubMedGoogle Scholar
  9. [9]
    Van Driessche E, Houf K, Vangroenweghe F, Nollet N, De Zutter L, Vandamme P and Van Hoof J (2004). Occurrence and strain diversity of Arcobacter species isolated from healthy Belgian pigs, Res. Microbiol. 155:662–666CrossRefPubMedGoogle Scholar
  10. [10]
    Houf K and Stephan R (2007). Isolation and charercterisation of the emerging food borne pathogen from human stool. J. Microbiol. Method 68: 408–413CrossRefGoogle Scholar
  11. [11]
    Russel RG, Kiehlbauch JA, Gebhart CJ, Detolla LJ (1992). Uncommon Campylobacter species in infant Macaca nemestrina monkeys housed in a nursery. J. Clin. Microbiol. 30:3024–3027Google Scholar
  12. [12]
    Higgins R, Messier S, Daignault D and Lorange M (1999). Arcobacter Arcobacter isolated from a diarrhoeic non-human primate. Lab. Anim. 33:87–90CrossRefPubMedGoogle Scholar
  13. [13]
    Fernandez H, Eller G, Paillacar J, Gajardo T and Riquelme A (1995). Toxigenic and invasive capacities: possible pathogenic mechanisms in Arcobacter cryaerophilus, Mem. Inst. Oswaldo. Cruz. 90:633–634CrossRefPubMedGoogle Scholar
  14. [14]
    Van Driesse E and K. Houf (2008). Survival capacity in water of Arcobacter species under different temperature conditions. Journal of Applied Microbiology 105(2):443–51CrossRefGoogle Scholar
  15. [15]
    Wesley IV, Wells SJ, Harmon KM, Green A, Schroeder-Tucker L, Glover M and Siddique I (20000) Fecal shedding of Campylobacter and Arcobacter spp. in dairy cattle, Appl. Environ. Microbiol. 66:1994–2000CrossRefPubMedGoogle Scholar
  16. [16]
    Troutt H F, Osburn B I. Meat from dairy cows: possible microbiological hazards and risks. Rev Sci Tech Off Int Epizoot. 1997; 16:405–414Google Scholar
  17. [17]
    Epoke J and Coker A. O (1991). Intestinal colonization of rats following experimental infection with Campylobacter jejuni. East African medical journal 68: 348–351PubMedGoogle Scholar

Copyright information

© © Versita Warsaw and Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  1. 1.Department of Medical Microbiology and Parasitology, College of Health SciencesLadoke Akintola University of TechnologyOsogboNigeria

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