Advertisement

Central European Journal of Medicine

, Volume 5, Issue 1, pp 108–114 | Cite as

Does diabetes affect the intensity of staining of interstitial cells and neuronal tissue in the bladder, prostate and urethra of rabbits?

  • Abdullah Erdem Canda
  • Safiye Aktas
  • Burak Turna
  • Mehtap G. Cinar
Research Article
  • 38 Downloads

Abstract

We compared the intensity of staining of interstitial cells (ICs) and neural tissue in the lower urinary tract of rabbits with diabetes with the intensity in normal subjects. Diabetes was induced by injecting alloxane (65mg/kg) in adult male rabbits. After 3 days, rabbits with a blood glucose level >300 mg/dL were considered to have diabetes. After 8 weeks, the rabbits were killed, and tissue specimens from the bladder, prostate and urethra were obtained. ICs were stained with anti-human CD117 (c-kit) rabbit polyclonal antibody, and neural tissue was stained with synaptophysin. The streptavidin-biotin method was used for immunohistochemical staining. The intensity of c-kit and synaptophysin staining were scored as negative (0), weak (+), moderate (++), and strong (+++). Staining intensity of ICs and neural tissue was assessed and compared in tissues obtained from rabbits with diabetes (n=8) and from control subjects (n=7). Although staining intensity of both ICs and neural tissue was found to be significantly decreased in the bladder tissue of rabbits with diabetes compared to that in the control group (p=0.0001 [ICs] and p=0.021 [neural tissue]), no significant differences in staining intensity of ICs and neural tissue in the urethra and in the prostate was found when rabbits with diabetes were compared to the control group. Diabetes may cause dysfunction of the lower urinary tract, particularly in the urinary bladder, as shown by the staining intensity of ICs and neural tissue.

Keywords

Diabetes Interstitial cells Neural tissue Bladder Prostate Urethra 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    Aubert R.E., Geiss L.S., Ballard D.J., Cocanougher B., Herman W.H., Diabetes related hospitalization and hospital utilization., In: Diabetes in America, 2nd Edition: National Institutes of Health; 553–569,1995Google Scholar
  2. [2]
    Poladia D.P., Schanbacher B., Wallace L.J., Bauer J.A., Innervation and connexin isoform expression during diabetes-related bladder dysfunction: early structural vs. neuronal remodeling, Acta Diabetol., 2005,42(3),147–152CrossRefPubMedGoogle Scholar
  3. [3]
    Kubota Y., Nakahara T., Mitani A., Maruko T., Sakamoto K., Ishii K., Augmentation of rat urinary bladder relaxation mediated by beta1-adrenoceptors in experimental diabetes, Eur J Pharmacol., 2003,467(1–3),191–195CrossRefPubMedGoogle Scholar
  4. [4]
    Su X., Changolkar A., Chacko S., Moreland R.S., Diabetes decreases rabbit bladder smooth muscle contraction while increasing levels of myosin light chain phosphorylation, Am J Physiol Renal Physiol., 2004,287(4),690–699CrossRefGoogle Scholar
  5. [5]
    Liu G., Lin Y.H., Yamada Y., Daneshgari F., External urethral sphincter activity in diabetic rats, Neurourol Urodyn., 2008,27(5),429–434CrossRefPubMedGoogle Scholar
  6. [6]
    Yang Z., Dolber P.C., Fraser M.O., Diabetic urethropathy compounds the effects of diabetic cystopathy, J Urol., 2007,178(5),2213–2219CrossRefPubMedGoogle Scholar
  7. [7]
    Torimoto K., Fraser M.O., Hirao Y., De Groat W.C., Chancellor M.B., Yoshimura N., Urethral dysfunction in diabetic rats, J Urol., 2004,171(5),1959–19564CrossRefPubMedGoogle Scholar
  8. [8]
    van der AA F., Roskams T., Blyweert W., Ost D., Bogaert G., De Ridder D., Identification of kit positive cells in the human urinary tract, J Urol., 2004,171(6 Pt 1),2492–2496Google Scholar
  9. [9]
    Wiseman O.J., Fowler C.J., Landon D.N., The role of the human bladder lamina propria myofibroblast, BJU Int., 2003,91(1),89–93CrossRefPubMedGoogle Scholar
  10. [10]
    Shafik A., El-Sibai O., Shafik A.A., Shafik I., Identification of interstitial cells of Cajal in human urinary bladder: concept of vesical pacemaker, Urology., 2004,64(4),809–813CrossRefPubMedGoogle Scholar
  11. [11]
    Sergeant G.P., Hollywood M.A., McCloskey K.D., Thornbury K.D., McHale N.G.,Specialised pacemaking cells in the rabbit urethra, J Physiol., 2000,526,359–366CrossRefPubMedGoogle Scholar
  12. [12]
    Seifert P., Benedic M., Effert P., Nerve fibers in tumors of the human urinary bladder, Virchows Arch., 2002,440(3),291–297CrossRefPubMedGoogle Scholar
  13. [13]
    Felkl M., Leube R.E., Interaction assays in yeast and cultured cells confirm known and identify novel partners of the synaptic vesicle protein synaptophysin, Neuroscience., 2008,156(2),344–352CrossRefPubMedGoogle Scholar
  14. [14]
    Wu J., Ohlsson M., Warner E.A., Loo K.K., Hoang T.X., Voskuhl R.R., et al., Glial reactions and degeneration of myelinated processes in spinal cord gray matter in chronic experimental autoimmune encephalomyelitis, Neuroscience., 2008,156(3),586–596CrossRefPubMedGoogle Scholar
  15. [15]
    Hermann G.G., Andersen C.B., Transitional cell carcinoma Express vitamin D receptors, Scand J Urol Nephrol., 1996,31,161–166CrossRefGoogle Scholar
  16. [16]
    Ristimaki A., Honkanen N., Jankala H., Sipponen P., Harkönen M., Expression of cyclooxygenase-2 in human gastric carcinoma, Cancer Res., 1997,57,1276–1280PubMedGoogle Scholar
  17. [17]
    Metzger R., Neugebauer A., Rolle U., Böhlig L., Till H., C-Kit receptor (CD117) in the porcine urinary tract, Pediatr Surg Int., 2008,24(1),67–76.CrossRefPubMedGoogle Scholar
  18. [18]
    Kubota Y., Hashitani H., Shirasawa N., Kojima Y., Sasaki S., Mabuchi Y., Altered distribution of interstitial cells in the guinea pig bladder following bladder outlet obstruction, Neurourol Urodyn., 2008,27(4),:330–340CrossRefPubMedGoogle Scholar
  19. [19]
    Højlund K., Mogensen M., Sahlin K., Beck-Nielsen H., Mitochondrial dysfunction in type 2 diabetes and obesity, Endocrinol Metab Clin North Am., 2008,37(3),713–731CrossRefPubMedGoogle Scholar
  20. [20]
    Abdul-Ghani M.A., DeFronzo R.A., Mitochondrial dysfunction, insulin resistance, and type 2 diabetes mellitus, Curr Diab Rep., 2008,8(3),173–178CrossRefPubMedGoogle Scholar
  21. [21]
    McCloskey KD., Characterization of outward currents in interstitial cells from the guinea pig bladder, J Urol., 2005,173,296–301PubMedCrossRefGoogle Scholar
  22. [22]
    Canda AE., Editorial Comment on: Molecular Mechanisms Related to Parturition-Induced Stress Urinary Incontinence, Eur Urol., 2008,Mar 18, [Epub ahead of print]Google Scholar
  23. [23]
    Canda A.E., Cross R.L., Chapple C.R., Pharmacology of the lower urinary tract and management of overactive bladder, J Turkish-German Gynecol Assoc., 2006,7,146–158Google Scholar
  24. [24]
    Canda A.E., Cinar M.G., Turna B., Sahin M.O., Pharmacologic targets on the female urethra, Urol Int., 2008,80(4),341–354CrossRefPubMedGoogle Scholar
  25. [25]
    Edwards F.R., Hirst G.D., Suzuki H., Unitary nature of regenerative potentials recorded from circular smooth muscle of guinea-pig antrum, J Physiol., 1999,519,235–250CrossRefPubMedGoogle Scholar
  26. [26]
    Sanders K.M., A case for interstitial cells of Cajal as pacemakers and mediators of neurotransmission in the gastrointestinal tract, Gastroenterology., 1996,111,492–515CrossRefPubMedGoogle Scholar
  27. [27]
    Collins C., Klausner A.P., Herrick B., Ko H.P., Miner A.S., Henderson S.C., Ratz P.H., Potential for control of detrusor smooth muscle spontaneous rhythmic contraction by cyclooxygenase products released by interstitial cells of Cajal, J Cell Mol Med., 2009, Feb 20, [Epub ahead of print]Google Scholar
  28. [28]
    Johnston L., Carson C., Lyons A.D., Davidson R.A., McCloskey K.D., Cholinergic-induced Ca2+ signaling in interstitial cells of Cajal from the guinea pig bladder, Am J Physiol Renal Physiol., 2008,294(3),645–655CrossRefGoogle Scholar
  29. [29]
    Lyons A.D., Gardiner T.A., McCloskey K.D., Kit-positive interstitial cells in the rabbit urethra: structural relationships with nerves and smooth muscle, BJU Int. 2007,99(3),687–694CrossRefPubMedGoogle Scholar
  30. [30]
    McCloskey K.D., Calcium currents in interstitial cells from the guinea-pig bladder, BJU Int., 2006,97(6),1338–1343CrossRefPubMedGoogle Scholar
  31. [31]
    Nobe K., Yamazaki T., Tsumita N., Hashimoto T., Honda K., Glucose-dependent enhancement of diabetic bladder contraction is associated with a rho kinase-regulated protein kinase C pathway, J Pharmacol Exp Ther., 2009,328(3),940–950CrossRefPubMedGoogle Scholar
  32. [32]
    Bradley W.E., Diagnosis of urinary bladder dysfunction in diabetes mellitus, Ann Intern Med 1980,92,323–326PubMedGoogle Scholar
  33. [33]
    Mitsui T., Kakizaki H., Kobayashi S., Morita H., Matsumura K., Koyanagi T., Vesicourethral function in diabetic patients: association of abnormal nerve conduction velocity with vesicourethral dysfunction, Neurourol Urodyn., 1999,18:639–645CrossRefPubMedGoogle Scholar
  34. [34]
    Andersson K.E., Treatment-resistant detrusor overactivity—underlying pharmacology and potential mechanisms, Int J Clin Pract Suppl., 2006,(151),8–16Google Scholar

Copyright information

© © Versita Warsaw and Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  • Abdullah Erdem Canda
    • 1
  • Safiye Aktas
    • 2
  • Burak Turna
    • 3
  • Mehtap G. Cinar
    • 4
  1. 1.1st Urology DepartmentAnkara Atatürk Training and Research HospitalAnkaraTurkey
  2. 2.Institute of Oncology, Department of Basic OncologyDokuz Eylül UniversityIzmirTurkey
  3. 3.Department of UrologyEge University School of MedicineIzmirTurkey
  4. 4.Department of Pharmacology and Clinical PharmacologyEge University, School of MedicineIzmirTurkey

Personalised recommendations