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Relationship of Anti-vimentin antibodies to anti-endothelial antibodies

  • Research Article
  • Published:
Central European Journal of Medicine

Abstract

The intermediate filament protein vimentin is a potential target antigen for autoantibodies in some infectious and autoimmune diseases. Because endothelial cells contain an extensive interconnecting cytoplasmic network of vimentin, we examined the relationship between anti-vimentin and anti-endothelial cell antibodies (AECAs). We measured the level of anti-vimentin antibodies in patients with systemic autoimmune diseases (n=42), healthy blood donors (n=58), and patients with acute inflammation and showing anti-vimentin immunofluorescence (n=50). AECAs were detected by indirect immunofluorescence in human umbilical vein endothelial cells. Anti-vimentin antibodies were detected by indirect immunofluorescence in HEp-2 cells, and levels of anti-vimentin antibodies were measured using an enzyme-linked immunosorbent assay. We did not find significant differences in anti-vimentin levels between AECA-positive and-negative samples for any of the groups; however, serum anti-vimentin antibody levels were significantly higher in patients with acute non-autoimmune inflammatory diseases than in patients with systemic autoimmune diseases or healthy controls. There were no differences between the levels of anti-vimentin antibodies between patients with systemic autoimmune diseases and healthy individuals. The anti-vimentin antibodies levels also did not correlate with the AECA positivity.

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Abbreviations

AECA:

anti-endothelial cell antibodies

EC:

endothelial cell

HUVEC:

human umbilical vein endothelial cell

IF:

intermediate filament

IIF:

indirect immunofluorescence

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Authors and Affiliations

  1. Institute of Immunology and Microbiology, General Faculty Hospital and First Faculty of Medicine, Charles University, 120 00, Prague, Czech Republic

    Karin Malickova, Ivana Janatkova & Terezie Fucikova

  2. Institute of Clinical Biochemistry and Laboratory Diagnostics, General Faculty Hospital and First Faculty of Medicine, Charles University, 120 00, Prague, Czech Republic

    Karin Malickova, Ivana Janatkova, Petra Sandova & Terezie Fucikova

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  1. Karin Malickova
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  2. Ivana Janatkova
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Malickova, K., Janatkova, I., Sandova, P. et al. Relationship of Anti-vimentin antibodies to anti-endothelial antibodies. cent.eur.j.med 1, 228–236 (2006). https://doi.org/10.2478/s11536-006-0028-5

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  • DOI: https://doi.org/10.2478/s11536-006-0028-5

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