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Production of hairy root cultures of lettuce (Lactuca sativa L.)

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Central European Journal of Biology

Abstract

Hairy root cultures of lettuce (Lactuca sativa L.) were obtained by inoculation of cotyledonary leaves of in vitro lettuce seedlings (cvs. Nansen and Ljubljanska ledenka) with Agrobacterium rhizogenes A4M70GUS. Approximately in 96.7% cvs. Nansen and in 91.2% Ljubljanska ledenka inoculated explants produced hairy root when they were incubated on Murashige and Skoog (MS) half-strength medium without plant growth regulators. A total of 54% of all hairy root cultures expressed GUS activity. Every hairy root represented an independent transformation event. Line Ljubljanska ledenka 18 showed the highest biomass (5.5 times the biomass of control root). A PCR analysis of the genomic DNA confirmed the presence of marker and target genes in 15 hairy roots examined.

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Abbreviations

GUS:

ß-glucuronidase

MS:

Murashige and Skoog medium

X-Gluc:

5-bromo-4-chloro-3-indolyl-ß-D-glucuronide

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Authors and Affiliations

  1. Department of Plant Physiology, Institute for Biological Research „Siniša Stanković“, University of Belgrade, 11060, Belgrade, Serbia

    Tadić Vojin, Milošević Snežana, Cingel Aleksandar, Petrić Marija, Trifunović Milana, Antonić Dragana & Subotić Angelina

  2. Department of Global Ecology, Carnegie Institution for Science, Stanford, CA, 94305, USA

    Tadić Jovan

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  1. Tadić Vojin
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  2. Milošević Snežana
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Vojin, T., Snežana, M., Aleksandar, C. et al. Production of hairy root cultures of lettuce (Lactuca sativa L.). cent.eur.j.biol. 9, 1196–1205 (2014). https://doi.org/10.2478/s11535-014-0351-9

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  • DOI: https://doi.org/10.2478/s11535-014-0351-9

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