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Central European Journal of Biology

, Volume 9, Issue 10, pp 915–921 | Cite as

Hydrogen peroxide induced by modulated electromagnetic radiation protects the cells from DNA damage

  • Andrew B. GapeyevEmail author
  • Nina A. Lukyanova
  • Sergey V. Gudkov
Research Article

Abstract

It is believed that non-ionizing electromagnetic radiation (EMR) and low-level hydrogen peroxide (H2O2) may change nonspecific resistance and modify DNA damage caused by ionizing radiation. To check this assumption, the combined effects of extremely high-frequency EMR (EHF EMR) and X-rays on induction of DNA damage in mouse whole blood leukocytes were studied. The cells were exposed to X-rays with or without preliminary treatment with EHF EMR or low-level H2O2. With the use of enhanced chemiluminescence, it was shown for the first time that pulse-modulated EHF EMR (42.2 GHz, incident power density of 0.1 mW/cm2, exposure duration of 20 min, modulation frequency of 1 Hz) induced H2O2 at a concentration of 4.6 ± 0.3 nM L−1 in physiological saline. With the use of an alkaline comet assay, it was found that the exposure of cells to the pulse-modulated EHF EMR, 25 min prior to treatment with X-rays at a dose of 4 Gy reduced the level of ionizing radiation-induced DNA damage. Continuous EHF EMR was inefficient. In turn, it was shown that low-level H2O2 (30–500 nM L−1) protected the cells against X-irradiation. Thus, the mechanisms of radiation protective effect of EHF EMR are connected with the induction of the adaptive response by nanomolar concentrations of reactive oxygen species formed by pulse-modulated EHF EMR.

Keywords

Extremely high-frequency electromagnetic radiation Pulse modulation X-rays Hydrogen peroxide DNA damage Comet assay Protective effect 

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Copyright information

© Versita Warsaw and Springer-Verlag Wien 2014

Authors and Affiliations

  • Andrew B. Gapeyev
    • 1
    • 2
    Email author
  • Nina A. Lukyanova
    • 1
    • 2
  • Sergey V. Gudkov
    • 2
    • 3
  1. 1.Institute of Cell Biophysics of Russian Academy of SciencesPushchino, Moscow RegionRussian Federation
  2. 2.Pushchino State Institute of Natural SciencesPushino, Moscow regionRussian Federation
  3. 3.Institute of Theoretical and Experimental Biophysics of Russian Academy of SciencesPushchino, Moscow RegionRussian Federation

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