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The relationship of glycerol and glycolysis metabolism patway under hyperosmotic stress in Dunaliella salina

  • Research Article
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Central European Journal of Biology

Abstract

This study detected the upstream and downstream key genes of glycolysis in Dunaliella Salina by using Real-time Fluorescence Quantitative PCR assays and measurement of enzyme activity. The results were as follows: the levels of transcription, enzyme activity, and protein of D. salina PFK were up-regulated under hyperosmotic stress while D. salina ENO were down-regulated. At the same time we monitored the change of intracellular degradation of starch, the synthesis of glycerol and PEP concentration in Dunaliella Salina under hyperosmotic stress. We found that lower expression of DsENO reduced the concentration of intracellular PEP which promoted the degradation of starch, and decreased the flow of carbon into the tricarboxylic acid cycle which would favor the synthesis of glycerol.

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Authors and Affiliations

  1. Key Laboratory of Bio-Resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, Sichuan, People’s Republic of China

    Bing-Bing Xia, Shuang-Hui Wang, Jing-Bo Duan & Lin-Han Bai

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  1. Bing-Bing Xia
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  2. Shuang-Hui Wang
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  4. Lin-Han Bai
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Correspondence to Lin-Han Bai.

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Xia, BB., Wang, SH., Duan, JB. et al. The relationship of glycerol and glycolysis metabolism patway under hyperosmotic stress in Dunaliella salina . cent.eur.j.biol. 9, 901–908 (2014). https://doi.org/10.2478/s11535-014-0323-0

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  • DOI: https://doi.org/10.2478/s11535-014-0323-0

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