Abstract
This article deals with the polarity of electrostatic charges that are carried on ballistic basidiospores after their liberation from fruiting bodies. The spores were collected by placing a portable device beneath the basidiome and allowing them to fall in a horizontal homogeneous electrostatic field, created by vertical parallel plane electrodes. Thus, the experimental setup enabled to investigate the primary charges, the charges present on spores immediately after the release from spore bearing cells. Spores of 135 basidiomes of 50 species of hymenomycetous fungi were collected in various natural conditions. The non-turgescent (drying up, collapsing or ceasing to sporulate) basidiomes were excluded from the taxonomical analysis; the 128 turgescent basidiomes (223 spore samples) of 47 species were taxonomically analyzed. These species represented eight orders (Agaricomycetes, Basidiomycota), covering 21 families and 36 genera. The analysis showed that the spore charges were distributed according to the polarity similarly in all samples in a species, and in a genus. In most cases also genera of one family had the same type of polarity distribution of spore charges. Possibly all taxa from species to monophyletic families are characterized by specific type of polarity of the primary electrostatic charge of basidiospores. Depending on the taxonomical group, all spore charges were negative, positive, or both negative and positive charges were present. This information could be useful in investing the ballistosporic discharge mechanism and for constructing higher-level phylogeny.
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Buller A.H.R., Researches on fungi, I, An account of the production, liberation, and dispersion of the spores of hymenomycetes treated botanically and physically, Also some observations upon the discharge and dispersion of the spores of ascomycetes and of Pilobolus, Longmans, Green & Co., London, 1909
Gregory P.H., Electrostatic charges on spores of fungi, Nature, 130, 330, 1957
Swinbank P., Taggart J., Hutchinson S.A., The measurement of the electrostatic charges on spores of Merulius lacrymans (Wulf.) Fr., Ann Bot-London, 28, 239–249, 1964
Gregory P.H., Microbiology of the atmosphere, 2nd ed., John Wiley & Sons, New York-Toronto, 1973
Webster J., Procton M.C.F., Davey R.A., Duller, D.A., Measurement of the electrical charge on some basidiospores and an assessment of two possible mechanisms of ballistospore propulsion, Trans Br Myc Soc, 91, 193–203, 1988
Clémençon H., Cytology and plectology of the Hymenomycetes. 2nd revised ed., J. Cramer, Stuttgart, 2012
Webster J., Davey R.A., Duller, D.A., Ingold C.T., Ballistospore discharge in Itersonilia perplexance, Trans Br Myc Soc, 82, 13–29, 1984
McLaughlin D.J., Beckett A., Yoon K.S., Ultrastructure and evolution of ballistosporic basidiospores, Bot J Lin Soc, 91(1–2), 253–271, 1985
Yoon K.S., McLaughlin D.J., Basidiosporogenesis in Boletus rubinellus, II, Late spore development, Mycologia 78(2), 185–197, 1986
Webster J., Davey R.A., Ingold C.T., Origin of the liquid in Buller’s drop, Trans Br Myc Soc, 83, 524–527, 1984
Goates B.J., Hoffmann J.A., Formation and discharge of secondary sporidia of the bunt fungus, Tilletia foetida, Mycologia, 78(3), 371–379, 1986
Webster J., Davey R.A., Turner J.C.R., Vapor as a source of water in Buller’s drop, Mycol Res, 93(3), 297–302, 1989
Webster J., Introduction to fungi, Cambridge University Press, 1970
Gregory P.H., Speculations on basidiospore liberation. Bulletin of the British Mycological Society, 13(2), 128–131, 1979
Seinfeld J. H., Pandis S. N., Atmospheric chemistry and physics: from air pollution to climate change, 2nd ed., Hoboken (N.J.): John Wiley & Sons, 2006
Stolze-Rybczynski J.L., Biomechanics of spore discharge in the Basidiomycota, PhD thesis, Miami University, USA, 2009
Money N.P., Fischer M.W.F, Biomechanics of spore release in phytopathogens, In: Deising H. (Ed.), Plant relationships, 2nd ed., The Mycota, V, Springer-Verlag, Berlin, Heidelberg, 2009
Saar M., Investigation of the electrostatic charge of basidiospores of the Phellinus igniarius group, Central European Journal of Biology, 8(5), 410–422, 2013
Saar M., Salm J., Emission rate of charged spores in basidiomycetous fungi and the relaxation time of their electric charges, Aerobiologia, 30(1), 71–89, 2014
Hibbett D.S., A phylogenetic overview of the Agaricomycotina, Mycologia, 98(6), 917–925, 2007
Matheny P.B., Curtis J.M., Hofstetter V. et al., Major clades of Agaricales: a multilocus phylogenetic overview, Mycologia 98(6), 982–995, 2007
Loeb L.B., Static electrification, Springer-Verlag, Berlin, 1958
Corner E.J.H., Studies in the basidium-sporespacing and the Boletus spores, The Garden’s Bulletin Singapore, 26, 159–194, 1972
Keller J., Contribution à la connaissance de l’infrastructure de la poroi sporique des Aphyllophorales, Thèse de doctorat, Université de Neuchătel, Suisse, 1974
Schaffert R.M., Electrophotography, Wiley & Sons, New York, 1975
Potapova T.V., Aslanidi K.B., Belozerskaya T.A., Levina N.N., Transcellular ionic studies by intracellular potential recordings in Neurospora crassa hyphae: Transfer of energy from proximal to apical cells, FEBS Letters, 241(1,2), 173–176, 1988
Belozerskaya T.A., Potapova T.V., Intrahyphal communication in segmented mycelium, Experimental Mycology, 17, 157–169, 1993
Belozerskaya T.A., Kritskii M.S., Sokolovskii V.Yu., Membrane electrogenesis and the problems of cell differentiation in mycelial fungi, Mikrobiologiya, 64(3), 293–300, 1995, (in Russian, with summary in English)
Belozerskaya T.A., Cell-to-cell communication in differentiation of mycelial fungi, Membrane & Cell Biology/Biologicheskie Membrany 11(6), 831, 1998, PMID 9718578
Belozerskaya T.A., Gessler N.N., Isakova E.P., Deryabina Yu.I., Neurospora crassa light signal transduction is affected by ROS, Journal of Signal Transduction, Vol. 2012, Art. ID 791963, 13 pages, 2012
Saar M., Noppel M., Salm, J., Enhancement of the collision efficiency between basidiospores and cloud droplets by electrostatic charges carried on freshly emitted basidiospores, European Aerosol Conference, Prague, 1st-6th September 2013, 2013
Buller A.H.R., Researches on fungi, II, Further investigations upon the production and liberation of spores in hymenomycetes, Longmans, Green & Co., London, 1922
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Saar, M., Parmasto, E. Primary basidiospore charge and taxonomy of Agaricomycetes. cent.eur.j.biol. 9, 874–887 (2014). https://doi.org/10.2478/s11535-014-0316-z
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DOI: https://doi.org/10.2478/s11535-014-0316-z