Central European Journal of Biology

, Volume 9, Issue 7, pp 668–677 | Cite as

Chemical composition, antimicrobial, antioxidative and anticholinesterase activity of Satureja Montana L. ssp montana essential oil

  • Tatjana Mihajilov-Krstev
  • Dragan Radnović
  • Dušanka Kitić
  • Vesna Stankov Jovanović
  • Violeta Mitić
  • Zorica Stojanović-RadićEmail author
  • Bojan Zlatković
Research Article


The present study investigates the chemical compositions of three Satureja montana L. ssp montana essential oils and correlates chemical variability with biological activities. GC/MS analysis showed that with an increase in altitude (100–500–800 m), a higher content of linalool, terpinen-4-ol and cis-sabinene hydrate was found, while the percentage of phenolic compounds, thymol and carvacrol decreased. Antimicrobial activity of the essential oils was tested against 7 fungal and 23 bacterial strains. The essential oil characterized by the highest content of phenols and alcohols exhibited the highest antimicrobial potential. The correlation analysis showed that the major carriers of the obtained antioxidant activity are oxygenated monoterpenes. All essential oils inhibited human serum cholinesterase activity. High antimicrobial potential, together with moderate antioxidant capacity and strong inhibition of human serum cholinesterase, classifies S. montana essential oil as a natural source of compounds that can be used in the treatment of foodborne and neurological diseases, wound and other infections, as well as for general health improvement.


Satureja montana L. ssp montana Essential oil Antimicrobial activity Antioxidant activity Cholinesterase inhibiton 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. [1]
    Redžić S., Wild edible plants and their traditional use in the human nutrition in Bosnia-Herzegovina, Free Radic. Res., 2006, 45, 189–232Google Scholar
  2. [2]
    Leporatti M.L., Ivancheva S., Preliminary comparative analysis of medicinal plants used in the traditional medicine of Bulgaria and Italy, J. Ethnopharmacol., 2003, 87, 123–142PubMedCrossRefGoogle Scholar
  3. [3]
    Zavatti M., Zanoli P., Benelli A., Rivasi M., Baraldi C., Baraldi M., Experimental study on Satureja montana as a treatment for premature ejaculation, J. Ethnopharmacol., 2011, 133, 2629–633CrossRefGoogle Scholar
  4. [4]
    Ćavar S., Maksimović M., Šolić M., Jerković-Mujkić E., Bešta R., Chemical composition and antioxidant and antimicrobial activity of two Satureja essential oils, Food Chem., 2008, 111, 648–653CrossRefGoogle Scholar
  5. [5]
    Radonić A., Miloš M., Chemical Composition and In Vitro Evaluation of Antioxidant Effect of Free Volatile Compounds from Satureja montana L., Free Radic. Res., 2003, 37, 673–679PubMedCrossRefGoogle Scholar
  6. [6]
    Skočibušić M., Bežić N., Chemical composition and antidiarrhoeal activities of winter savory (Satureja montana L.) Essential Oil, Pharm. Biol., 2003, 41, 622–626CrossRefGoogle Scholar
  7. [7]
    Skočibušić M., Bežić N., Chemical composition and antimicrobial variability of Satureja montana L. essential oils produced during ontogenesis, J. Essent. Oil. Res., 2004, 16, 387–391CrossRefGoogle Scholar
  8. [8]
    Oussalah M., Caillet S., Saucier L., Lacroix M., Inhibitory effects of selected plant essential oils on the growth of four pathogenic bacteria: E. coli O157:H7, Salmonella typhimurium, Staphylococcus aureus and Listeria monocytogenes, Food Control, 2007, 18, 414–420CrossRefGoogle Scholar
  9. [9]
    Carramiñana J.J., Rota C., Burillo J., Herrera A., Antibacterial efficiency of spanish Satureja Montana essential oil against Listeria monocytogenes among natural flora in minced pork, J. Food Protect, 2008, 71, 502–508Google Scholar
  10. [10]
    Nedorostova L., Kloucek P., Kokoska L., Stolcova M., Pulkrabek J., Antimicrobial properties of selected essential oils in vapor phase against foodborne bacteria, Food Control, 2009, 20, 157–160CrossRefGoogle Scholar
  11. [11]
    Ciani M., Menghini L., Mariani F., Pagiotii R., Menghini A., Fatichenti F., Antimicrobial properties of essential oil of Satureja montana L., on pathogenic and spoilage yeasts, Biotechnol. Lett., 2000, 22, 1007–1010CrossRefGoogle Scholar
  12. [12]
    Lampronti I., Saab A.M., Gambari R., Antiproliferative activity of essential oils derived from plants belonging to the Magnoliophyta division, Int. J. Oncol., 2006, 29, 989–995PubMedGoogle Scholar
  13. [13]
    Prieto J.M., Iacopini P., Cioni P., Chericoni S., In vitro activity of the essential oils of Origanum vulgare, Satureja montana and their main constituents in peroxynitrite-induced oxidative processes, Food Chem., 2007, 104, 889–895CrossRefGoogle Scholar
  14. [14]
    Slavkovska V., Jančić J., Bojović S., Milosavljević S., Đoković D., Variability of essential oils of Satureja montana L. and Satureja kitaibelii Wierzb. ex Heuff. from the central part of the Balkan peninsula, Phytochem., 2001, 57, 71–76CrossRefGoogle Scholar
  15. [15]
    Miloš M., Radonić A., Bežić N., Dunkić V., Localities and seasonal variations in the chemical composition of essential oils of Satureja montana L. and S. cuneifolia Ten., Flavour. Fragr. J., 2001, 16, 157–160Google Scholar
  16. [16]
    Ibraliu A., Dhillon B.S., Faslia N., Stich B., Variability of essential oil composition in Albanian accessions of Satureja montana L., J. Med. Plants. Res., 2010, 4, 1359–1364Google Scholar
  17. [17]
    Mastelić J., Jerković I., Gas chromatography-mass spectrometry analysis of free and glycoconjugated aroma compounds of seasonally collected Satureja montana L., Food Chem., 2003, 80, 135–140CrossRefGoogle Scholar
  18. [18]
    Šilić Č., Monograph of the genera Satureja L. Calamintha Miller, Micromeria Bentham, Acinos Miller and Clinopodium L. in flora of Yugoslavia [Monografija rodova Satureja L. Calamintha Miller, Micromeria Bentham, Acinos Miller i Clinopodium L. u flori Jugoslavije], Zemaljski Muzej BiH, Sarajevo, 1979Google Scholar
  19. [19]
    Bežić N., Šamanić I., Dunkić V., Besendorfer V., Puizina J., Essential Oil Composition and internal transcribed spacer (ITS) sequence variability of four south-Croatian Satureja species (Lamiaceae), Molecules, 2009, 14, 925–938PubMedCrossRefGoogle Scholar
  20. [20]
    Clevenger J.P., Content o essential oil in plants, American Perfumer and Essential Oil Review, 1928, 23, 467–503Google Scholar
  21. [21]
    Adams R.P., Identification of essential oil components by gas hromatography/mass spectrometry. 4th Ed., Allured Publishing Corporation, Carol Stream, IL, 2007Google Scholar
  22. [22]
    Mihajilov-Krstev T., Radnović D., Kitić D., Zlatković B., Ristić M., Branković S., Chemical composition and antimicrobial activity of Satureja hortensis L. essential oil., Cent. Eur. J. Biol., 2009, 4, 411–416CrossRefGoogle Scholar
  23. [23]
    Kulišić T., Radonić A., Katalinić V., Miloš M., Use of different methods for testing antioxidative activity of oregano essential oil, Food Chem., 2004, 85, 633–640CrossRefGoogle Scholar
  24. [24]
    Stojanović G., Stojanović I., Stankov-Jovanović V., Mitić V., Kostić D., Reducing power and radical scavenging activity of four Parmeliaceae species, Cent. Eur. J. Biol., 2010, 5, 808–813CrossRefGoogle Scholar
  25. [25]
    Re R., Pellegrini N., Proteggente A., Pannula A., Yang M., Rice-Evans C., Antioxidant activity applying an improved abts radical cation decolorization assay, Free Radic. Biol. Med., 1999, 26, 1231–1237PubMedCrossRefGoogle Scholar
  26. [26]
    Sanchez-Moreno C., Methods used to evaluate the free radical scavenging activity in foods and biological systems, Food Sci. Technol. Int., 2002, 8, 121–137CrossRefGoogle Scholar
  27. [27]
    Stankov-Jovanović V.P., Nikolić-Mandić S.D., Mandić Lj.M., Mitić V.D., Modification of the kinetic determination of pancuronium bromide based on its inhibitory effect on cholinesterase, J. Clin.Lab. Anal., 2007, 21, 124–131PubMedCrossRefGoogle Scholar
  28. [28]
    Hadian J., Tabatabaei S.M.F., Naghavi M.R., Jamzad Z., Ramak-Masoumi T., Genetic diversity of Iranian accessions of Satureja hortensis L. based on horticultural traits and RAPD markers, Sci. Hortic., 2008, 115, 196–202CrossRefGoogle Scholar
  29. [29]
    Dunkić V., Bezić N., Vuko E., Cukrov D., Antiphytoviral Activity of Satureja montana L. ssp. variegata (Host) P. W. Ball Essential Oil and Phenol Compounds on CMV and TMV, Molecules, 2010, 15, 6713–6721CrossRefGoogle Scholar
  30. [30]
    Angelini L.G., Carpanese G., Cioni P.L., Morelli I., Macchia M., Flamini G., Essential oils from Mediterranean Lamiaceae as weed germination inhibitors, J. Agric. Food Chem., 2003, 51, 6158–6164PubMedCrossRefGoogle Scholar
  31. [31]
    Nikaido H., Prevention of drugs access to bacterial targets: permeability barriers and active efflux, Science, 1994, 264, 382–388PubMedCrossRefGoogle Scholar
  32. [32]
    Dorman H.J.D., Deans S.G., Antimicrobial agents from plants: antibacterial activity of plant volatile oils, J. App. Microbiol., 2000, 88, 308–316CrossRefGoogle Scholar
  33. [33]
    Zhou F., Ji B., Zhang H., Jiang H., Yang Z., Li J., Li J., Yan W., The antibacterial effect of cinnamaldehyde, thymol, carvacrol and their combinations against the foodborne pathogen Salmonella typhimurium, J. Food Safety, 2007, 27, 124–133CrossRefGoogle Scholar

Copyright information

© Versita Warsaw and Springer-Verlag Wien 2014

Authors and Affiliations

  • Tatjana Mihajilov-Krstev
    • 1
  • Dragan Radnović
    • 2
  • Dušanka Kitić
    • 3
  • Vesna Stankov Jovanović
    • 4
  • Violeta Mitić
    • 4
  • Zorica Stojanović-Radić
    • 1
    Email author
  • Bojan Zlatković
    • 1
  1. 1.Faculty of Science and Mathematics, Department of Biology and EcologyUniversity of NišNišSerbia
  2. 2.Department of Biology and EcologyUniversity of Novi SadNovi SadSerbia
  3. 3.Faculty of Medicine, Department of PharmacyUniversity of NišNišSerbia
  4. 4.Faculty of Science and Mathematics, Department of ChemistryUniversity of NišNišSerbia

Personalised recommendations