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Skin extracellular matrix components accelerate the regenerative potential of Lin− cells

  • Research Article
  • Published:
Central European Journal of Biology

Abstract

Due to their unique properties, bone marrow-derived Lin− cells can be used to regenerate damaged tissues, including skin. The objective of our study was to determine the influence of the skin tissue-specific microenvironment on mouse Lin− cell proliferation and migration in vitro. Cells were analyzed for the expression of stem/progenitor surface markers by flow cytometry. Proliferation of MACS-purified cells in 3D cultures was investigated by WST-8 assay. Lin− cell migration was evaluated by in vitro scratch assay. The results obtained show that basement membrane matrix is more effective for Lin− cell proliferation in vitro. However, type I collagen matrix better enhances the re-epithelization process, that depends on the cell migratory properties. These studies are important for preparing cells to be used in transplantation.

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Authors and Affiliations

  1. Division of Stem Cell Biology, State Research Institute Center for Innovative Medicine, LT-01102, Vilnius, Lithuania

    Giedrė Ramanauskaitė, Dovilė Žalalytė, Vytautas Kašėta, Aida Vaitkuvienė & Genė Biziulevičienė

  2. Department of Biotechnology and Microbiology, Faculty of Natural Sciences, Vilnius University, LT-03101, Vilnius, Lithuania

    Lilija Kalėdienė & Genė Biziulevičienė

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  1. Giedrė Ramanauskaitė
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  2. Dovilė Žalalytė
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  3. Vytautas Kašėta
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  4. Aida Vaitkuvienė
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  5. Lilija Kalėdienė
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  6. Genė Biziulevičienė
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Correspondence to Genė Biziulevičienė.

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Ramanauskaitė, G., Žalalytė, D., Kašėta, V. et al. Skin extracellular matrix components accelerate the regenerative potential of Lin− cells. cent.eur.j.biol. 9, 367–373 (2014). https://doi.org/10.2478/s11535-013-0283-9

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  • DOI: https://doi.org/10.2478/s11535-013-0283-9

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