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Central European Journal of Biology

, Volume 9, Issue 4, pp 374–382 | Cite as

QTL mapping for germination of seeds obtained from previous wheat generation under drought

  • Ilona Czyczyło-MyszaEmail author
  • Izabela Marcińska
  • Edyta Skrzypek
  • Katarzyna Cyganek
  • Katarzyna Juzoń
  • Małgorzata Karbarz
Research Article

Abstract

The QTLs controlling germination and early seedling growth were mapped using seeds acquired from mapping population and parental lines of Chinese Spring and SQ1 grown under water-limited conditions, severe drought (SDr) and well-watered plants (C). Germination ability was determined by performing a standard germination test based on the quantification of the germination percentage (GP24) of seeds incubated for 24 h at 25°C in the dark. Early seedling growth was evaluated on the basis of the length of the root and leaf at the 6th day of the experiment. QTLs were identified by composite interval mapping method using Windows QTLCartographer 2.5 software. For the traits studied, a total of thirty eight additive QTLs were identified. Seventeen QTLs were mapped in C on chromosomes: 1A, 2A, 7A, 1B, 2B, 3B, 4B, 5B, 6B, 7B, 2D, 3D, 4D and 6D, while twenty one QTLs were identified in SDr on chromosomes: 1A, 2A, 5A, 2B, 3B, 4B, 5B, 6B, 7B, 3D, 5D and 6D. Most of the QTLs for GP and early leaf growth parameters were clustered on chromosome 4B (associated with the Rht-B1 marker) both in C and SDr plants. The results indicate the complex and polygenic nature of germination.

Keywords

Germination Leaf Root Quantitative trait loci Triticum aestivum L. 

Abbreviations

QTL

quantitative trait loci

DH

double haploid

C

control (well-watering)

SDr

severe drought

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Copyright information

© Versita Warsaw and Springer-Verlag Wien 2013

Authors and Affiliations

  • Ilona Czyczyło-Mysza
    • 1
    Email author
  • Izabela Marcińska
    • 1
  • Edyta Skrzypek
    • 1
  • Katarzyna Cyganek
    • 1
  • Katarzyna Juzoń
    • 1
  • Małgorzata Karbarz
    • 2
    • 3
  1. 1.The F. Górski Institute of Plant PhysiologyPolish Academy of SciencesKrakówPoland
  2. 2.Institute of Applied Biotechnology and Basic SciencesUniversity of RzeszowKolbuszowaPoland
  3. 3.Centre of Applied Biotechnology and Basic SciencesUniversity of RzeszowKolbuszowaPoland

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