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Mediated amperometry reveals two distinct modes of yeast responses to glucose

  • Research Article
  • Published:
Central European Journal of Biology

Abstract

Mediated amperometry was exploited to monitor intracellular redox activity without cell disruption. Continuous measurements of menadione-mediated glucose currents at carbon paste electrodes with various immobilized intact wild type yeasts (Saccharomyces cerevisiae, Candida pulcherrima, Clavispora lusitaniae, Wickerhamomyces anomalus, Pichia guilliermondii, Kluyveromyces lactis var. lactis, Debaryomyces hansenii, Candida zeymolaydes and Candida tropicalis) revealed two distinct and previously unreported modes of development of the currents during the first 2 to 3 min. after subjection to glucose. A correlation among the values of the currents and the capacities of wild type yeasts to secrete various substances was observed.

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Authors and Affiliations

  1. Center for Physical Sciences and Technology, Institute of Chemistry, 01108, Vilnius, Lithuania

    Rasa Garjonyte & Albertas Malinauskas

  2. Nature Research Center, Institute of Botany, 08406, Vilnius, Lithuania

    Vytautas Melvydas, Algimantas Paškevičius & Valerijus Rašomavičius

Authors
  1. Rasa Garjonyte
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  2. Vytautas Melvydas
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  3. Algimantas Paškevičius
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  4. Valerijus Rašomavičius
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  5. Albertas Malinauskas
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Correspondence to Rasa Garjonyte.

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Garjonyte, R., Melvydas, V., Paškevičius, A. et al. Mediated amperometry reveals two distinct modes of yeast responses to glucose. cent.eur.j.biol. 9, 173–181 (2014). https://doi.org/10.2478/s11535-013-0257-y

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  • DOI: https://doi.org/10.2478/s11535-013-0257-y

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