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Candidate plant gene homologues in grapevine involved in Agrobacterium transformation

  • Research Article
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Central European Journal of Biology

Abstract

The grapevine (Vitis vinifera) genome was analyzed in silico for homologues of plant genes involved in Agrobacterium transformation in Arabidopsis thaliana and Nicotiana spp. Grapevine homologues of the glucomannan 4-betamannosyltransferase 9 gene CslA-09 involved in bacterial attachment to the cell wall, homologues of reticulon-like proteins BTI1, 2, 3 and RAB8 GTPases, both involved in T-DNA transfer to the host cell, homologues of VirE2 interacting protein VIP1 that contributes to the targeting of T-DNA into the nucleus and to its integration, and homologues of the histone protein H2A, which promotes the expression of T-DNA encoded genes, were selected. Sequences homologous to the arabinogalactan-protein AtAGP17 were not found in the grape genome. Seventeen selected candidates were tested by semiquantitative RT-PCR analysis for changes in their expression levels upon inoculation with Agrobacterium tumefaciens C58. Of the tested homologues, the expression of VvRab8a, VvVip1a and two histone genes (VvHta2 and VvHta10) increased significantly, therefore we hypothesize that these might be involved in Agrobacterium transformation of V. vinifera.

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Authors and Affiliations

  1. Department of Viticulture, Corvinus University of Budapest, Institute for Viticulture and Enology, 1118, Budapest, Hungary

    Tamás Deák, Tünde Kupi & György D. Bisztray

  2. Department of Genetics and Plant Breeding, Corvinus University of Budapest, Faculty of Horticultural Sciences, 1118, Budapest, Hungary

    Róbert Oláh

  3. Department of Dermatology and Allergology, University of Szeged, 6720, Szeged, Hungary

    Lóránt Lakatos & Lajos Kemény

  4. Experimental Station of Kecskemét, Corvinus University of Budapest, Institute for Viticulture and Enology, 6000, Kecskemét-Katonatelep, Hungary

    Ernő Szegedi

Authors
  1. Tamás Deák
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  2. Tünde Kupi
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  3. Róbert Oláh
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  4. Lóránt Lakatos
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  5. Lajos Kemény
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  6. György D. Bisztray
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  7. Ernő Szegedi
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Correspondence to György D. Bisztray or Ernő Szegedi.

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Deák, T., Kupi, T., Oláh, R. et al. Candidate plant gene homologues in grapevine involved in Agrobacterium transformation. cent.eur.j.biol. 8, 1001–1009 (2013). https://doi.org/10.2478/s11535-013-0218-5

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  • DOI: https://doi.org/10.2478/s11535-013-0218-5

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