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Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum

  • Research Article
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Central European Journal of Biology

Abstract

Soybean [Glycine max (L.) Merr.] cultivars (Meli, Alisa, Sava and 1511/99) were grown up to V1 phase (first trifoliate and one node above unifoliate) and then inoculated with Sclerotinia sclerotiorum (Lib.) de Bary under controlled conditions. Changes in L-phenylalanine ammonia-lyase (PAL) activity and isoflavone phytoalexins were recorded 12, 24, 48 and 72 h after the inoculation. Results showed an increase in PAL activity in all four examined soybean cultivars 48 h after the inoculation, being the highest in Alisa (2-fold higher). Different contents of total daidzein, genistein, glycitein and coumestrol were detected in all samples. Alisa and Sava increased their total isoflavone content (33.9% and 6.2% higher than control, respectively) as well as 1511/99, although 48 h after the inoculation its content decreased significantly. Meli exhibited the highest rate of coumestrol biosynthesis (72 h after the inoculation) and PAL activity (48 h after the inoculation). All investigated cultivars are invariably susceptible to this pathogen. Recorded changes could point to possible differences in mechanisms of tolerance among them.

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Authors and Affiliations

  1. Faculty of Agriculture, University of Novi Sad, 21000, Novi Sad, Serbia

    Djordje Malenčić & Biljana Kiprovski

  2. Department of Pharmacy, Faculty of Medicine, University of Novi Sad, 21000, Novi Sad, Serbia

    Jelena Cvejić, Vesna Tepavčević & Mira Bursać

  3. Institute of Field and Vegetable Crops, 21000, Novi Sad, Serbia

    Miloš Rajković

Authors
  1. Djordje Malenčić
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  2. Jelena Cvejić
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  3. Vesna Tepavčević
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  4. Mira Bursać
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  5. Biljana Kiprovski
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  6. Miloš Rajković
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Correspondence to Djordje Malenčić.

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Malenčić, D., Cvejić, J., Tepavčević, V. et al. Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum . cent.eur.j.biol. 8, 921–929 (2013). https://doi.org/10.2478/s11535-013-0201-1

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  • DOI: https://doi.org/10.2478/s11535-013-0201-1

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