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Induction of hairy roots in Arnica montana L. by Agrobacterium rhizogenes

  • Research Article
  • Published:
Central European Journal of Biology

Abstract

The induction of hairy roots in Arnica montana L. by Agrobacterium rhizogenes mediated system was established. The frequency of genetic transformation varied from 4.8 to 12% depended on method of infection. The cefotaxime at concentration of 200 mg/l proved to suppress effectively the growth of A. rhizogenes after co-cultivation. Among the three tested nutrient media: Murashige and Skoog (MS), Gamborg’s (B5) and Schenk and Hildebrandt (SH), MS medium was superior for growth and high biomass production of transformed roots compared to other culture media. After culturing for 40 days the fresh weight of clone T4 increased 7.6 fold over the non-transformed roots. The transfer of rol A, rol B and rol C genes into Arnica genome was confirmed by PCR analysis. Established genetic transformation techniques in A. montana efficiently provided and generated a large number of transformed roots — an excellent system for studying gene function and could be used for the production of secondary metabolites synthesized in roots.

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Abbreviations

MS:

Murashige and Skoog medium

B5:

Gamborg’s medium

SH:

Schenk and Hildebrandt medium

PCR:

polymerase chain reaction

MP:

micropropagated plants

RP:

regenerated plants from callus culture

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Authors and Affiliations

  1. Department of Applied Genetics and Plant Biotechnology, Bulgarian Academy of Sciences, Institute of Plant Physiology and Genetics, Sofia, 1113, Bulgaria

    Mariya Petrova & Ely Zayova

  2. Department of Functional Genetics, Bulgarian Agricultural Academy, AgroBioInstitute, Sofia, 1164, Bulgaria

    Mariana Vlahova

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  1. Mariya Petrova
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  2. Ely Zayova
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  3. Mariana Vlahova
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Correspondence to Mariya Petrova.

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Petrova, M., Zayova, E. & Vlahova, M. Induction of hairy roots in Arnica montana L. by Agrobacterium rhizogenes . cent.eur.j.biol. 8, 470–479 (2013). https://doi.org/10.2478/s11535-013-0157-6

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  • DOI: https://doi.org/10.2478/s11535-013-0157-6

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