Abstract
The goal of this work was to determine the best method of breaking the achene dormancy in Potamogeton praelongus Wulfen. The ways of breaking achene dormancy studied in this experiment included methods of achene storage, stratification, UVA radiation, anaerobic conditions, mechanical disruption of achenes?outer layers and their chemical disruption by NaClO. Nine different treatments of achenes were combined with two methods of achene storage. Particular achene treatments and storage conditions were proven to have a significant impact on breaking dormancy. Although the highest germination rate (83.3%) was achieved when the dormancy was broken chemically by long effect of 100% concentrations of Savo detergent (containing 5% NaClO), the growth of the sprouts was subsequently inhibited due to toxic effects of Savo. Thus the most successful treatment was based on changing temperature, e.g. 2.5 months of cold storage followed by 14 days at room temperature (germination rate 32.7%). This treatment was also most similar to the natural process. Germinated achenes were also found in Petri dishes exposed to UVA radiation, anaerobic conditions and chemical disruption of the outer layers. Results of these treatments were influenced by the storage method.
Similar content being viewed by others
References
Vöge M., Development of Potamogeton praelongus in Grossensee lake near Hamburg [Die Entwicklung von Potamogeton praelongus im Grossensee bei Hamburg], Tuexenia, Göttingen, 1992, 12, 275–284 (in German)
Kaplan Z., Potamogeton L. — rdest, In: Chrtek J. Jr., Kaplan Z., Štěpánková J. (Eds), Květena České republiky 8. Vyd. 1., Academia, Prague, 2010
Koizumi Y., Hara Y., Yazaki Y., Sakano K., Ishizawa K., Involvement of plasma membrane H+-ATPase in anoxic elongation of stems in pondweed (Potamogeton distinctus) turions, New Phytologist., 2011, 190, 421–430
Martin A.C., The comparative internal morphology of seeds, Am. Midl. Nat., 1946, 36, 513–660
Goebel K., Organography of plants, Part II, Clarendon Press, Oxford, 1905
Dahlgren R.M.T., Clifford H.T., The Monocotyledons A Comperative Study, Academic Press, New York, 1982
[7] BaskinC.C., Baskin J.M., Seeds: Ecology, biogeography, and evolution of dormancy and germination, Academic Press, San Diego, 1998
Santamaria L., Charalambidou I., Figuerola J., Green A.J., Effect of passage through duck gut on germination of fennel pondweed seeds, Arch Hydrobiol, 2002, 156, 11–22
Waycott M., Assessment of genetic variation and clonality in the seagrass Posidonia australis using RAPD and allozyme analysis, Mar. Ecol. Prog. Ser., 1995, 116, 289–295
Loveless M.D., Hamrick J.L., Ecological determinants of genetic structure in plant populations, Ann. Rev. Ecol. Syst., 1984, 15, 65–69
Prausová R., Janová J., Adamec L., Rescue of the critically endangered long stalked pondweed (Potamogeton praelongus) in the Czech Republic, Acta Biologica Slovenica, 2011, 54, 43–54
Janová J., Long stalked pondweed (Potamogeton praelongus WULFEN) in the Czech Republic [Rdest dlouholistý (Potamogeton praelongus WULFEN) v České republice], MSc thesis, University Hradec Králové, Czech Republic, 2010, (in Czech)
Hintze J., NCSS 2001 — Number Cruncher Statistical System, Kaysville, Utah, USA, 2001
Less D.H., Breeding systems, population structure, and evolution in hydrophilous angiosperms, Ann Mo Bot Gard, 1988, 75, 819–835
Muenscher W.C., The germination of seeds of Potamogeton, Ann. Bot., 1936, 50, 805–821
Wehrmeister J.R., An ecological life history of the pondweed Potamogeton crispus L. in North America, MSc thesis, Ohio State University, Columbus, Ohio, 1978
Rodgers K.H., Breen C.M., Growth and reproduction of Potamogeton crispus in a South African lake, J. Ecol., 1980, 68, 561–571
Teltscherová L., Hejný S., The Germination of Some Potamogeton Species from South Bohemian Fishponds, Folia Geobot. Phytotax, 1973, 8, 231–239
Hay F., Probert R., Dawson M., Laboratory germination of seeds from 10 British species of Potamogeton, Aquat Bot, 2008, 88, 353–357
Waisel Y., Seasonal activity and reproductive behaviour of some submerged hydrophytes in Israel, Hydrobiologia, 1971, 12, 219–227.
Brux H., Toeskino D., Wiegleb G., Growth and reproduction of Potamogeton alpinus, Arch Hadrobiol. Beih. Ergebn. Limnol., 1987, 27, 115–127
Fischer A., Hydrogen and hydroxyl such conditions germination [Wasserstoff- und hydroxylionen als Keimungsreize], Ber. Deutsch. Bot. Gesells., 1907, 15, 108–122, (in German)
Crocker W., Germination of Seeds of Water Plants, Bot. Gaz., 1907, 375–380
Prausová R., Adamec L., Kitner M., Pásek K., Dvořák V., Janová J., et al., Rescue of the long stalked pondweed (Potamogeton praelongus) in the Czech Republic [Záchrana rdestu dlouholistého (Potamogeton praelongus) v České republice], Final report, Agency for Nature Protection of the Czech Republic, Prague, 2010, (in Czech)
Guppy H.B., Germination of seed of aquatic plants, Proc. Royal Phys. Soc. Edinb., 1894–1897, 13, 344–360
Farokh P., Mahmoodzadeh H., Satari T.N., Response of Seed Germination of Safflower to UV-B radiation, RJES, 2010, 4, 70–74
Schulz M.R., Klein R.M., Effects of visible and ultraviolet radiation on the germination of Phacelia tanacetifolia, Amer. Jour. Bot., 1963, 50, 430–434
Tosserams M., Bolink E., Rozema J., The effect of anhanced ultraviolet-B radiation on germination and seedling development of plant species occurring in a dune grassland ecosystem, Plant Ecol, 1997, 128, 139–147
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Prausová, R., Janová, J. & Šafářová, L. Testing achene germinationof Potamogeton praelongus Wulfen. cent.eur.j.biol. 8, 78–86 (2013). https://doi.org/10.2478/s11535-012-0114-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.2478/s11535-012-0114-4