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A rapid microtitre plate Folin-Ciocalteu method for the assessment of polyphenols

Central European Journal of Biology volume 8, pages 48–53 (2013)Cite this article

Abstract

Several methods have been described for the determination of phenolic compounds in animal and plant products using the Folin-Ciocalteu (FC) assay. Most of these methods describe the use of this reagent and sodium carbonate in spectrophotometric methods. The macro FC assay was compared with two micro FC assays carried out on a microplate reader. Excellent correlation was obtained among the three assays with a molar extinction coefficient of 5.228±0.187x103 M−1 cm−1. The micro assay may serve as a high throughput method for the rapid determination of polyphenols in various samples.

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Authors and Affiliations

  1. Institute of Earth Systems, Division of Rural Sciences and Food Systems, University of Malta, MSD 2080, Msida, Malta

    Everaldo Attard

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  1. Everaldo Attard
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Correspondence to Everaldo Attard.

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Attard, E. A rapid microtitre plate Folin-Ciocalteu method for the assessment of polyphenols. cent.eur.j.biol. 8, 48–53 (2013). https://doi.org/10.2478/s11535-012-0107-3

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  • DOI: https://doi.org/10.2478/s11535-012-0107-3

Keywords

  • UV-VIS spectrophotometry
  • Microplate reader
  • Methanolic extracts
  • Tannic acid