Skip to main content
Log in

Total phenolic content, flavonoid concentration, antioxidant and antimicrobial activity of methanol extracts from three Seseli L. taxa

  • Research Article
  • Published:
Central European Journal of Biology


The present study describes the total phenolic content, concentrations of flavonoids and in vitro antioxidant and antimicrobial activity of methanol extracts from Seseli pallasii Besser, S. libanotis (L.) Koch ssp. libanotis and S. libanotis (L.) Koch ssp. intermedium (Rupr.) P. W. Ball, growing wild in Serbia. The total phenolic content in the extracts was determined using Folin-Ciocalteu reagent and their amounts ranged between 84.04 to 87.52 mg GA (gallic acid)/g. The concentrations of flavonoids in the extracts varied from 4.75 to 19.37 mg Qu (quercetin)/g. Antioxidant activity was analyzed using DPPH reagent. Antioxidant activity ranged from 0.46 to 4.63 IC50 (mg/ml) and from 1.98 to 2.19 mg VitC (vitamin C)/g when tested with the DPPH and ABTS reagents, respectively, using BHA and VitC as controls. The antimicrobial activity of the extracts was investigated using a micro-well dilution assay for the most common human gastrointestinal pathogenic bacterial strains: Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 9027, Salmonella enteritidis ATCC 13076, Bacillus cereus ATCC 10876, Listeria monocytogenes ATCC15313, Staphylococcus aureus ATCC 25923 and Candida albicans ATCC 10231. This finding suggests that Seseli species may be considered as a natural source of antioxidants and antimicrobial agents.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others


  1. Nikolić V., Genus Seseli, In: Josifović M. (Ed.), Flora of Serbia 5, Belgrade, SANU, 1973

    Google Scholar 

  2. Ball P.W., Genus Seseli L., In: Tutin T.G., Heywood V.H., Burges N.A., Moore D.M., Valentine D.H., Walters S.M., Webb D.A. (Eds.), Flora Europaea 2, Cambridge University Press, London, 1968

    Google Scholar 

  3. Pavela R., Screening of Eurasian plants for insecticidal and growth inhibition activity against Spodoptera littoralis larvae, Afr. J. Agric. Res., 2011, 6, 2895–2907

    Google Scholar 

  4. Ozturk A., Ozturk S., Kartal, S., The characteristics and uses of herbs added to herby cheeses in Van, Herb J. Syst. Bot., 2000, 7, 167–181

    Google Scholar 

  5. Baytop T., Treatment with Plants in Turkey, Addition, 2nd ed., Nobel Medical Publications, Ankara, 1999

    Google Scholar 

  6. Ozturk S., Ercisli S., Chemical composition and in vitro antibacterial activity of Seseli libanotis, World J. Microbiol. Biotechnol., 2006, 22, 261–265

    Article  CAS  Google Scholar 

  7. Milosavljević S., Tesević V., Vucković I., Jadranin M., Vajs V., Soković M., et al., Composition and antifungal activity of the essential oil of Seseli annuum wildgrowing in Serbia, Fitoterapia., 2007, 78, 319–322

    Article  PubMed  Google Scholar 

  8. Janaćković P., Soković M., Vujisić L., Vajs V., Vucković I., Krivosej Z., et al., Composition and antimicrobial activity of Seseli globiferum essential oil, Nat. Prod. Commun., 2011, 6, 1163–1166

    PubMed  Google Scholar 

  9. Siljegović J., Glamoclija J., Soković M., Vucković I., Tesević V., Milosavljević S., et al., Composition and antimicrobial activity of Seseli montanum subsp. ommasinii essential oil, Nat. Prod. Commun., 2011, 6, 263–266

    PubMed  Google Scholar 

  10. Stojković S., Petrović S., Kukić J., Dzamić A., Ristić M., Milenković M., et al., Chemical composition and antimicrobial and antioxidant activity of Seseli rigidum flower essential oil, Chem. Nat. Comp., 2007, 45, 253–256

    Article  Google Scholar 

  11. Arnao M.B., Some methodological problems in the determination of antioxidant activity using chromogen radicals: A practical case, Trends Food Sci. Tech., 2000, 11, 419–421

    Article  CAS  Google Scholar 

  12. Singleton V.L., Orthofer R., Lamuela-Raventos R.M., Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent, Meth Enzymol., 1999, 299, 152–178

    Article  CAS  Google Scholar 

  13. Woisky R., Salatino A., Analysis of propolis: some parameters and procedures for chemical quality control, J. Apic. Res., 1998, 37, 99–105

    CAS  Google Scholar 

  14. Blois M.S., Antioxidant determinations by the use of a stable free radical, Nature., 1958, 181, 1199–1200

    Article  CAS  Google Scholar 

  15. Miller N., Rice-Evans C., Factors influencing the antioxidant activity determined by the ABTS radical cation assay, Free Radic Res., 1997, 26, 195–199

    Article  PubMed  CAS  Google Scholar 

  16. NCCLS — National Committee for Clinical Laboratory Standards, Performance standards for anti-microbial susceptibility testing: eleventh informational supplement, Document M100-S11, National Committee for Clinical Laboratory Standard, Wayne, PA, USA, 2003

    Google Scholar 

  17. Sartoratto A., Machado A.L.M., Delarmelina C., Figueira G.M., Duarte M.C.T., Rehder V.L.G., Composition and antimicrobial activity of essential oils from aromatic plants used in Brazil, Braz. J. Microbiol., 2004, 35, 275–280

    Article  CAS  Google Scholar 

  18. Stobiecki M., Kachlicki P., Isolation and identification of flavonoids, In: Grotewold E. (Ed.), The Science of Flavonoids, 2nd ed., Springer Science and Business Media, New York, 2006

    Google Scholar 

  19. Molyneux P., The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity, Songklanakarin J. Sci. Technol., 2004, 26, 211–219

    CAS  Google Scholar 

  20. Tosun A., Ozkal N., Yildiz S., Antimicrobial activity screening of some Seseli L. species growing in Turkey, J. Fac. Pharm., 2004, 33, 151–155

    Google Scholar 

  21. Seyd M., Chaudhary F.M., Bhatty M.K., Antimicrobial activity of Essential Oils of Umbelliferae Family. Part III. Seseli libanotis, Ligusticum stewartii and Pycnocycla aucheriana Oils, Pak. J. Sci. Ind. Res., 1989, 32, 316–319

    Google Scholar 

  22. Meene S., Chaudhary F.M., Bhatty M.K., Antimicrobial activity of seeds of Seseli libanotis, Pak. J. Sci. Ind. Res., 1989, 32, 316–319

    Google Scholar 

Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to Petar D. Marin.

About this article

Cite this article

Matejić, J.S., Džamić, A.M., Mihajilov-Krstev, T. et al. Total phenolic content, flavonoid concentration, antioxidant and antimicrobial activity of methanol extracts from three Seseli L. taxa. cent.eur.j.biol. 7, 1116–1122 (2012).

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: