Central European Journal of Biology

, Volume 7, Issue 4, pp 648–654 | Cite as

Glucose inhibits the shoot bud formation in the moss Bryum billarderi

  • Arturo Martínez Zavala
  • Netzahualcoyotl Mayek Pérez
  • Analilia Arroyo Becerra
  • Miguel Angel Villalobos López


Plant development is controlled by certain factors such as nutrient availability, environmental cues and the presence of signalling molecules. It has been proposed that phytohormones interact with sugars to modulate important processes in vascular plants. Cytokinins are key hormones because they regulate a large number of metabolic events, and sugars act as regulatory signals at several points in the life cycle. Bryum bilarderi Schwägr is a moss that was isolated by our group in the central highlands of Mexico and has demonstrated the ability to tolerate abiotic stresses. To study the effect of cytokinins and their interaction with glucose in bud induction, different concentrations of cytokinins with glucose were tested. One micromolar N-6-benzylaminopurine provided the best results for bud induction, but when 100 mM glucose was added, bud formation was inhibited. This glucose concentration also favoured the spread of the protonemal colony. These data demonstrate that N-6-benzylaminopurine is more effective than kinetin in inducing buds, and that glucose plays an important role as an inhibitory signalling molecule for the bud induction process that is mediated by cytokinins in the moss B. billarderi.


Bryophyte developmental biology Bud formation Bryum billarderi Cytokinins Glucose 









Murashige-Skoog medium


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  1. [1]
    Gibson S.I., Sugar and phytohormone response pathways: navigating a signalling network, J. Exp. Bot., 2004, 55, 253–264PubMedCrossRefGoogle Scholar
  2. [2]
    Inoue T., Higuchi M., Hashimoto Y., Seki M., Kobayashi M., Kato T., et al., Identification of CRE1 as a cytokinin receptor from Arabidopsis, Nature, 2001, 409, 1060–1063PubMedCrossRefGoogle Scholar
  3. [3]
    Gibson S.I., Control of plant development and gene expression by sugar signalling, Curr. Opin. Plant Biol., 2005, 8, 93–102PubMedCrossRefGoogle Scholar
  4. [4]
    Cove D.J., Knight C.D., The moss Physcomitrella patens, a model system with potential for the study of plant reproduction, Plant Cell, 1993, 5, 1483–1488PubMedGoogle Scholar
  5. [5]
    Cove D.J., Bezanilla M., Harries P., Quatrano R.S., Mosses as model systems for the study of metabolism and development, Ann. Rev. Plant Biol., 2006, 57, 497–520CrossRefGoogle Scholar
  6. [6]
    Decker E.L., Frank W., Sarnighausen E., Reski R., Moss systems biology en route: phytohormones in Physcomitrella development, Plant Biol., 2006, 8, 397–405PubMedCrossRefGoogle Scholar
  7. [7]
    Cove D.J., The moss Physcomitrella patens, Ann. Rev. Genet., 2005, 39, 339–358PubMedCrossRefGoogle Scholar
  8. [8]
    Brandes H., Kende H., Studies on cytokinin-controlled bud formation in moss protonemata, Plant Physiol., 1968, 43, 827–837PubMedCrossRefGoogle Scholar
  9. [9]
    Reski R., Abel W.O., Induction of budding on chloronemata and caulonemata of the moss, Physcomitrella patens, using isopentenyladenine, Planta, 1985, 165, 354–358CrossRefGoogle Scholar
  10. [10]
    Christianson M.L., The quantitative response to cytokinin in the moss Funarya hygrometrica does not reflect differential sensitivity of initial target cells, Am. J. Bot., 1998, 85, 144–148PubMedCrossRefGoogle Scholar
  11. [11]
    Thelander M., Olsson T., Ronne H., Effect of the energy supply on filamentous growth and development in Physcomitrella patens, J. Exp. Bot., 2005, 56, 653–662PubMedCrossRefGoogle Scholar
  12. [12]
    Murashige T., Skoog F., A revised medium for rapid growth and bioassays with tobacco tissue culture, Physiol. Plant., 1962, 15, 473–497CrossRefGoogle Scholar
  13. [13]
    Christianson M.L., Hornbuckle J.S., Phenylurea cytokinins assayed for induction of shoot buds in the moss Funaria hygrometrica, Am. J. Bot., 1999, 86, 1645–1648PubMedCrossRefGoogle Scholar
  14. [14]
    Christianson M.L., ABA prevents the second cytokinin-mediated event during the induction of shoot buds in the moss Funaria hygrometrica, Am. J. Bot., 2000, 87, 1540–1545PubMedCrossRefGoogle Scholar
  15. [15]
    Rolland F., Winderickx J., Thevelein J.M., Glucosesensing mechanisms in eukaryotic cells, Trends Biochem. Sci., 2001, 26, 310–317PubMedCrossRefGoogle Scholar
  16. [16]
    Rolland F., Moore B., Sheen J., Sugar sensing and signalling in plants, Plant Cell, 2002, 14, S185–S205PubMedGoogle Scholar
  17. [17]
    Sheen J., C4 gene expression, Ann. Rev. Plant Physiol. Plant Mol. Biol., 1999, 50, 187–217CrossRefGoogle Scholar
  18. [18]
    Olsson T., Thelander M., Ronne H., A novel type of chloroplast stromal hexokinase is the major glucosephosphorylating enzyme in the moss Physcomitrella patens, J. Biol. Chem., 2003, 278, 44439–44447PubMedCrossRefGoogle Scholar
  19. [19]
    Heintz D., Erxleben A., High A.A., Wurtz V., Reski R., Van Dorsselaer A., et al., Rapid alteration of the phosphoproteome in the moss Physcomitrella patens after cytokinin treatment, J. Proteome Res., 2006, 5, 2283–2293PubMedCrossRefGoogle Scholar
  20. [20]
    Harvey B.M.R., Lu B.C., Fletcher R.A., Benzyladenine accelerates chloroplast differentiation and stimulates photosynthetic enzyme activity in cucumber cotyledons, Can. J. Bot., 1974, 52, 2581–2586CrossRefGoogle Scholar
  21. [21]
    Feierabend J., Boer J., Comparative analysis of the action of cytokinin and light on the formation of ribulosebisphosphate carboxylase and plastid biogenesis, Planta, 1978, 142, 75–82CrossRefGoogle Scholar
  22. [22]
    Chen C.M., Leisner S.M. Cytokinin-modulated gene expression in excised pumpkin cotyledons, Plant Physiol., 1985, 77, 99–103PubMedCrossRefGoogle Scholar
  23. [23]
    Stitt M., von Schaewen A., Willmitzer L., “Sink” regulation of photosynthetic metabolism in transgenic tobacco plants expressing yeast invertase in their cell wall involves a decrease of the Calvin-cycle enzymes and an increase of glycolytic enzymes, Planta, 1990, 183, 40–45Google Scholar
  24. [24]
    Krapp A., Quick W.P., Stitt M., Ribulose-1,5-bisphosphate carboxylase-oxygenase, other Calvin-cycle enzymes, and chlorophyll decrease when glucose is supplied to mature spinach leaves via the transpiration stream, Planta, 1991, 186, 58–69CrossRefGoogle Scholar
  25. [25]
    Sheen J., Metabolic repression of transcription in higher plants, Plant Cell, 1990, 2, 1027–1038PubMedGoogle Scholar
  26. [26]
    Badenoch-Jones J., Parker C.W., Letham D.S., Singh S., Effect of cytokinins supplied via the xylem at multiples of endogenous concentrations on transpiration and senescence in derooted seedlings of oat and wheat, Plant, Cell & Environ., 1996, 19, 504–516CrossRefGoogle Scholar
  27. [27]
    Wingler A., von Schaewen A., Leegood R.C., Lea P.J., Quick W.P., Regulation of leaf senescence by cytokinin, sugars, and light. Effects on NADHdependent hydroxypyruvate reductase, Plant Physiol., 1998, 116, 329–335CrossRefGoogle Scholar

Copyright information

© Versita Warsaw and Springer-Verlag Wien 2012

Authors and Affiliations

  • Arturo Martínez Zavala
    • 1
  • Netzahualcoyotl Mayek Pérez
    • 2
  • Analilia Arroyo Becerra
    • 1
  • Miguel Angel Villalobos López
    • 1
  1. 1.National Polytechnic InstituteCenter of Applied Biotechnology ResearchTepetitlaMexico
  2. 2.National Polytechnic InstituteGenomics Biotechnology CenterReynosaMexico

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