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Unusual increase of Scd1 and Elovl6 expression in rat inguinal adipose tissue

  • Research Article
  • Published:
Central European Journal of Biology

Abstract

It is generally accepted that the location of body fat deposits may play an important role in the risk of developing some endocrine and metabolic diseases. We have studied the effect of food restriction and food restriction/refeeding, often practiced by individuals trying to lose body weight, on the expression of genes which are associated with obesity and certain metabolic disorders in inguinal, epididymal, and perirenal rat white adipose tissues. Gene expression was analyzed by real time semi-quantitative polymerase chain reaction and by Western blot. We found that prolonged food restriction caused a significant decrease of body and adipose tissue mass as well as the increase of Scd1 and Elovl6 gene expressions in all main rat adipose tissue deposits. Food restriction/refeeding caused increases of: a) Scd1 and Elovl6 mRNA levels in adipose tissue, b) Scd1 protein level and c) desaturation index in adipose tissue. The increased expression of both genes was unusually high in inguinal adipose tissue. The results suggest that the increase of Scd1 and Elovl6 gene expressions in white adipose tissue by prolonged food restriction and prolonged food restriction/refeeding may contribute to accelerated fat recovery that often occurs in individuals after food restriction/refeeding.

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Authors and Affiliations

  1. Department of Biochemistry, Medical University of Gdańsk, 80-211, Gdańsk, Poland

    Jacek Turyn & Julian Swierczynski

  2. Faculty of Biology, University of Gdańsk, 80-680, Gdańsk, Poland

    Adriana Mika

  3. Institute for Environmental and Human Health Protection, University of Gdańsk, 80-952, Gdańsk, Poland

    Piotr Stepnowski

Authors
  1. Jacek Turyn
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  2. Adriana Mika
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  3. Piotr Stepnowski
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  4. Julian Swierczynski
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Correspondence to Julian Swierczynski.

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Turyn, J., Mika, A., Stepnowski, P. et al. Unusual increase of Scd1 and Elovl6 expression in rat inguinal adipose tissue. cent.eur.j.biol. 7, 192–200 (2012). https://doi.org/10.2478/s11535-012-0007-6

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  • DOI: https://doi.org/10.2478/s11535-012-0007-6

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