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Nutritional regulation of adipose tissue lipoprotein lipase is blunted in insulin resistant rats

  • Research Article
  • Published:
Central European Journal of Biology

Abstract

Background

Hypertriglyceridemia is a common lipid abnormality accompanying insulin resistance. This study was designed to assess the contribution of dysregulation of adipose tissue lipoprotein lipase (LPL) activity to the hypertriglyceridemia in a rat model of insulin resistance.

Methodology

Hereditary hypertriglyceridemic (HHTg) rats were challenged for two weeks on a high sucrose diet and LPL activity, angptl-4 expression and FFA utilisation in vitro were determined in adipose tissue.

Results

Compared to control rats (Wistar), HHTg rats exhibited hyperinsulinemia, impaired fatty acid storage in adipose tissue and elevated LPL activity both in fasting and after refeeding. The expression of angiopoietin-like protein 4 (angptl4), a fastinginduced control protein for LPL activity, was not increased in adipose tissue of fasted HHTg rats as it was in the control rats.

Conclusion

We conclude that LPL remains in its active form to a higher extent in HHTg rat adipose tissue due to the low expression of angptl4 on fasting. This is a possible consequence of the hyperinsulinemia. In combination with the impaired storage of fatty acids as triglycerides in adipose tissue in HHTg rats, the inflexibility of angptl4 expression may contribute to the establishment of hypertriglyceridemia in the insulin-resistant animals.

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Authors and Affiliations

  1. Department of Metabolism and Diabetes, Institute for Clinical and Experimental Medicine, 140 21, Prague, Czech Republic

    Monika Cahova, Zuzana Papackova, Eliska Palenickova, Helena Dankova, Jana Zdychova & Ludmila Kazdova

  2. Faculty of Food and Biochemical Technology, Institute for Chemical Technology, 166 28, Prague, Czech Republic

    Vojtech Skop

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  1. Monika Cahova
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  2. Zuzana Papackova
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  3. Eliska Palenickova
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  4. Helena Dankova
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  6. Vojtech Skop
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  7. Ludmila Kazdova
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Correspondence to Monika Cahova.

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Cahova, M., Papackova, Z., Palenickova, E. et al. Nutritional regulation of adipose tissue lipoprotein lipase is blunted in insulin resistant rats. cent.eur.j.biol. 7, 201–209 (2012). https://doi.org/10.2478/s11535-012-0002-y

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  • DOI: https://doi.org/10.2478/s11535-012-0002-y

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