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Thyroid hormonal axis regulates protein C anticoagulation pathway in rats

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Central European Journal of Biology

Abstract

Effects of the hormones of the hypothalamic-pituitary-thyroid axis on some basic parameters of the activity of protein C anticoagulation pathway in rats are studied. Thyrotropin-releasing hormone (0.06 mg/kg body mass), thyrotropin (1 IU/kg), triiodothyronine (T3) (0.08 mg/kg), thyroxine (T4) (0.08 mg/kg), administered subcutaneously for three consecutive days on four different groups of rats increased significantly activated protein C, free protein S and protein S activity, and reduced the soluble endothelial protein C receptor. Protein C antigen and total protein S were significantly elevated only by thyrotropin-releasing hormone and thyroid-stimulating hormone, but they were not affected by T3 and T4 treatment. The data indicate the hypothalamic-pituitary-thyroid axis is involved in the regulation of the protein C anticoagulation pathway in rats by activation of this system, suggesting a tendency of hypocoagulability.

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Abbreviations

TRH:

Thyrotropin releasing hormone

TSH:

Thyrotropin (thyroid-stimulating hormone)

T3:

Trijodthyronin

T4:

Thyroxine

EPCR:

endothelial protein C receptor

sEPCR:

soluble form of endothelial protein C receptor

PC:

protein C

aPC:

activated protein C

References

  1. Castellino F.J., Ploplis V.A., The protein C pathway and pathologic processes, J. Thromb. Haemost., 2009, 7, 140–145

    Article  PubMed  CAS  Google Scholar 

  2. Dahlback B., Blood coagulation and its regulation by anticoagulant pathways: genetic pathogenesis of bleeding and thrombotic diseases, J. Int. Med., 2005, 257, 209–223

    Article  Google Scholar 

  3. Tanaka K., Nigel S.K., Jerrold H.L., Blood Coagulation: Hemostasis and Thrombin Regulation, Anesth. Analg., 2009, 108, 1433–1446

    Article  PubMed  CAS  Google Scholar 

  4. Joyce D.E., Gelbert L., Ciaccia A., DeHoff B., Grinnell B.W., Gene expression profile of antithrombotic protein C defines new mechanisms modulating inflammation and apoptosis, J. Biol. Chem., 2001, 276, 11199–11203

    Article  PubMed  CAS  Google Scholar 

  5. Minami T., Sugiyama A., Wu S.Q., Abid R., Kodama T., Aird W.C., Thrombin and phenotypic modulation of the endothelium, Arterioscler. Thromb. Vasc. Biol., 2004, 24, 41–53

    Article  PubMed  CAS  Google Scholar 

  6. Erem C., Ersoz H.O., Karti S.S., Ukinc K., Hacihasanoglu A., Deger O., et al., Blood coagulation and fibrinolysis in patients with hyperthyroidism, J. Endocrinol. Invest., 2002, 25, 345–350

    PubMed  CAS  Google Scholar 

  7. Canturk Z., Cetinarslan B., Tarkun I., Canturk N.Z., Ozden M., Duman C., Hemostatic system as a risk factor for cardiovascular disease in women with subclinical hypothyroidism, Thyroid, 2003, 13, 971–977

    Article  PubMed  Google Scholar 

  8. Gullu S., Sav H., Kamel N., Effects of levothyroxine treatment on biochemical and hemostasis parameters in patients with hypothyroidism, Eur. J. Endocrinol., 2005, 152, 355–361

    Article  PubMed  CAS  Google Scholar 

  9. Franchini M., Hemostatic changes in thyroid diseases, Haemost. Thromb., Hematol., 2006, 11, 203–208

    CAS  Google Scholar 

  10. Debeij J, Cannegieter S., Van Zaane B., Smit J., Corssmit E., Rosendaal F., et al., The effect of changes in thyroxine and thyroid-stimulating hormone levels on the coagulation system, J. Thromb. Haemost., 2010, 8, 2823–2826

    Article  PubMed  CAS  Google Scholar 

  11. Zhang W., Tian L., Han Y., Ma H., Wang L., Guo J., et al., Presence of thyrotropin receptor in hepatocytes: not a case of illegitimate transcription, J. Cell. Mol. Med., 2009, 13, 4636–4642

    Article  PubMed  CAS  Google Scholar 

  12. Yen P., Physiological and molecular basis of thyroid hormone action, Physiol. Rev., 2001, 81, 1097–1142

    PubMed  CAS  Google Scholar 

  13. Lifschitz B., Defesi C., Surks M., Thyrotropin response to thyrotropin-releasing hormone in the euthyroid rat: Dose-response, time course, and demonstration of partial refractoriness to a second dose of thyrotropin-releasing hormone, Endocrinology, 1978, 102, 1775–1782

    Article  PubMed  CAS  Google Scholar 

  14. Huff M., Culm-Merdek K., Fogle R., Gotschall R., Sampath K., Sendak R., et al., Pharmacokinetics and pharmacodynamics following low dose TSH administration in aged ovariectomized rats, FASEB J., 2007, 21, 5711–5713

    Google Scholar 

  15. Greeley G., Lipton M., Kizer J., Serum Thyroxine, Triiodothyronine, and TSH levels and TSH Release after TRH in aging male and female rats, Endocr. Res. Commun., 1982–1983, 9, 169–177

    Article  PubMed  Google Scholar 

  16. Negrev N.N., Radev R.Z., Velikova M.S., Anogeianaki A., Screening study of the effects of the hypothalamic-pituitary-thyroid axis on hemostasis in rats, J. Biol. Regul. Homeostat. Agent., 2006, 20, 53–57

    CAS  Google Scholar 

  17. España F., Vagà A., Mira Y., Medina P., Estellés A., Villa P., et al., Low level of circulating activated protein C is a risk factor for venous thromboembolism, Thromb. Haemost., 2001, 86, 1368–1373

    PubMed  Google Scholar 

  18. Drury, R.A.B., Wallington E.A., Carleton’s histological technique, 5th Ed., Oxford University Press, Oxford, UK, 1980

    Google Scholar 

  19. Esmon C.T., The protein C pathway, Chest, 2003, 124, 26S–32S

    Article  PubMed  CAS  Google Scholar 

  20. Van de Wouwer M., Collen D., Conway E.M., Thrombomodulin-protein C-EPCR system: integrated to regulate coagulation and inflammation, Arterioscler. Thromb. Vasc. Biol., 2004, 24, 1374–1383

    Article  PubMed  Google Scholar 

  21. Liaw P.C.Y, Neuenschwander P.F., Smirnov M.D., Esmon C.T., Mechanisms by which soluble endothelial cell protein C receptor modulates protein C and activated protein C function, J. Biol. Chem., 2000, 275, 5447–5452

    Article  PubMed  CAS  Google Scholar 

  22. Castoldi E., Hackeng T.M., Regulation of coagulation by protein S, Curr. Opin. Hematol., 2008, 15, 529–536

    Article  PubMed  CAS  Google Scholar 

  23. Rosing J., Maurissen L.F.A., Tchaikovski S.N., Tans G., Hackeng T.M., Protein S is a cofactor for tissue factor pathway inhibitor, Thromb. Res., 2008, 122, S60–S63

    Article  PubMed  CAS  Google Scholar 

  24. Negrev N.N., Radev R.Z., Velikova M.S., Anogeianaki A., Effects of the hormones of the thyroid axis on the vitamin K-dependent plasma factors of blood coagulation (II, VII, IX and X), Int. J. Immunopathol. Pharmacol., 2008, 21, 221–226

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Physiology, Medical University, 9300, Varna, Bulgaria

    Negrin Negrev, Margarita Stefanova & Emiliya Stancheva

  2. Department of Physiology, Medical University, 1432, Sofia, Bulgaria

    Yuri Nyagolov

Authors
  1. Negrin Negrev
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  2. Yuri Nyagolov
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  3. Margarita Stefanova
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  4. Emiliya Stancheva
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Correspondence to Yuri Nyagolov.

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Negrev, N., Nyagolov, Y., Stefanova, M. et al. Thyroid hormonal axis regulates protein C anticoagulation pathway in rats. cent.eur.j.biol. 6, 518–523 (2011). https://doi.org/10.2478/s11535-011-0031-y

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  • DOI: https://doi.org/10.2478/s11535-011-0031-y

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