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Central European Journal of Biology

, Volume 5, Issue 6, pp 853–863 | Cite as

Bulgarian golden root in vitro cultures for micropropagation and reintroduction

  • Krasimira Tasheva
  • Georgina KosturkovaEmail author
Research Article
  • 160 Downloads

Abstract

Rhodiola rosea is an endangered medicinal plant used for cancer, cardiovascular, and nervous system diseases therapy. Due to its limited distribution and sustainability alternative methods for production of its valuable substances are under investigation. Using in vitro techniques apical and rhizome buds, leaf nodes, stem and radix segments from wild plants and in vitro seedlings were plated on 24 modified Murashige and Skoog (1962) media. Decontamination of plant material was successful only in 21% of the schemes. The best shoot induction was obtained from seedling explants on media containing 2 mg/l zeatin or N6-benzylaminopurine, each. Their reduction stimulated shoot formation in the next passages (multiplication rate up to 5). Efficient rooting was induced on half-strength MS with 2 mg/l Indole-3-butyric acid and stimulated by adding 0.2 mg/l Indolyl-3-acetic acid. Regenerants rooted in perlite, peat, and soil (1:1:2), adapted in greenhouse, and transplanted in the mountains survived (70%) and developed like the wild plants. Salidroside content of these plants after one or two years was high (0.64 and 0.61% in rhizomes and 0.62 and 0.53% in roots, respectively). This is the first established efficient scheme for micropropagation of Bulgarian R. rosea allowing habitats restoration, germplasm conservation, and potential application of biotechnology for production of valuable substances.

Keywords

Rhodiola rosea Golden root Medicinal herb In vitro response Micropropagation Germplasm conservation Salidroside 

Abbreviations

BAP

N6-benzylaminopurine

GA3

Gibberellic acid

IAA

Indolyl-3-acetic acid

IBA

Indole 3-butyric acid

Kin

Kinetin

MS

Murashige and Skoog medium, 1962

NAA

α- naphthyl acetic acid

PFD

photon flux density

TDZ

Thidiazuron

2-iP

6-(y,y-dimethylallyl amino) purine

2,4-D

2,4- dichlorophenoxyacetic acid

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Copyright information

© © Versita Warsaw and Springer-Verlag Wien 2010

Authors and Affiliations

  1. 1.Institute of GeneticsBulgarian Academy of SciencesSofiaBulgaria

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