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Antihemolytic and antioxidant activities of Allium paradoxum

Central European Journal of Biology volume 5, pages 338–345 (2010)Cite this article

Abstract

Antioxidant activity of the aerial part and bulbs of Allium paradoxum was investigated by eight in vitro assay systems. Extracts showed good antioxidant activity. IC50 for 1,1-diphenyl-2-picryl hydrazyl radical-scavenging activity was 890.9±43.2 and 984.9±33.5 µg/ml for the aerial part and bulbs, respectively. The aerial parts have better reducing power than bulb extracts but not comparable with Vitamin C (P>0.001). Extracts showed weak Fe2+ chelating ability, the IC50 being 959±47 and 530±24 µg/ml for bulbs and aerial parts, respectively. Both tested extracts exhibited good hydrogen peroxide scavenging in a concentration dependent manner. They exhibited good antioxidant activity against the hemoglobin-induced linoleic acid system that was comparable with vitamin C (P>0.01). They showed good activity against cumene hydro peroxide induced hemolysis in RBCs. In addition, they possessed antihemolytic activity. The extract from aerial parts had significantly higher total phenol and flavonoid content than did bulbs. Amounts of eight elements (Cu, Mn, Zn, Fe, Ni, Pb, Cd and Cr) were also determined in the bulb and aerial part using atomic absorption spectroscopy. They contained higher Fe and Mn contents than other elements.

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References

  1. Cadenas E., Davies K.J.A., Mitochondrial free radical generation, oxidative stress, and aging, Free Radic. Biol. Med., 2000, 29, 222–230

    Article  CAS  PubMed  Google Scholar 

  2. Marnett L., Oxyradicals and DNA damage, Carcinogenesis, 2000, 21, 361–370

    Article  CAS  PubMed  Google Scholar 

  3. Burits M., Bucar F., Antioxidant activity of Nigella sativa essential oil, Phytother. Res., 2000, 14, 323–328

    Article  CAS  PubMed  Google Scholar 

  4. Kalt W., Forney C., Martin A., Prior R.L., Antioxidant capacity, vitamin C, phenolics, and anthocyanins after fresh storage of small fruits, J. Agric. Food Chem., 1999, 47, 4638–4644

    Article  CAS  PubMed  Google Scholar 

  5. Kumari M., Gupta S., Lakshmi A., Prakash J., Iron bioavailability in green leafy vegetables cooked in different utensils, Food Chem., 2004, 86, 217–222

    Article  CAS  Google Scholar 

  6. Leterme P., Buldgen A., Estrada F., Londono A.M., Mineral content of tropical fruits and unconventional foods of the Andes and the rain forest of Colombia, Food Chem., 2006, 95, 644–652

    Article  CAS  Google Scholar 

  7. Milton K., Micronutrient intakes of wild primates: are humans different?, Comp. Biochem. Phys. A, 2003, 136, 47–59

    Article  Google Scholar 

  8. Mozaffarian V., A dictionary of Iranian plant names, Tehran, Farhang Moaser, 2006

    Google Scholar 

  9. Krest I., Glodek J., Keusgen M., Cysteine sulfoxides and alliinase activity of some allium species, J. Agric. Food Chem., 2000, 48, 3753–3760

    Article  CAS  PubMed  Google Scholar 

  10. Han C., Li J., Hui Q., Determination of trace elements in Jinqi, a traditional chinese medicine, Biol. Trace Elem. Res., 2008, 122, 122–126

    Article  CAS  PubMed  Google Scholar 

  11. Ebrahimzadeh M.A., Pourmorad F., Hafezi S., Antioxidant activities of Iranian Corn Silk, Turk. J. Biol., 2008, 32, 43–49

    CAS  Google Scholar 

  12. Singleton V.L., Orthofer R., Lamuela-Raventos R.M., Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin Ciocalteu reagent, Meth. Enzymol., 1999, 299, 152–178

    Article  CAS  Google Scholar 

  13. Ghasemi K., Ghasemi Y., Ebrahimzadeh M.A., Antioxidant activity, phenol and flavonoid contents of 13 Citrus species peels and tissues, Pak. J. Pharm. Sci., 2009, 22, 277–281

    CAS  PubMed  Google Scholar 

  14. Chang C., Yang M., Wen H., Chern J., Estimation of total flavonoid content in propolis by two complementary colorimetric methods, J. Food Drug Anal., 2002, 10, 178–182

    CAS  Google Scholar 

  15. Nabavi S.M., Ebrahimzadeh M.A., Nabavi S.F., Fazelian M., Eslami B., In vitro antioxidant and free radical scavenging activity of Diospyros lotus and Pyrus boissieriana growing in Iran, Pharmacognosy Magazine, 2009, 4, 123–127

    Google Scholar 

  16. Yamaguchi T., Takamura H., Matoba T., Terao Y., HPLC method for evaluation of the free radical-scavenging activity of food by using 1,1-diphenil,-2-picrylhydrazil, Biosci. Biotechnol. Biochem., 1998, 62, 1201–1204

    Article  CAS  PubMed  Google Scholar 

  17. Nabavi S.M., Ebrahimzadeh M.A., Nabavi S.F., Hamidinia A., Bekhradnia A.R., Determination of antioxidant activity, phenol and flavonoids content of Parrotia persica Mey, Pharmacologyonline, 2008, 2, 560–567

    Google Scholar 

  18. Yen G.C., Chen H.Y., Antioxidant activity of various tea extracts in relation to their antimutagenicity, J. Agr. Food Chem., 1995, 43, 27–32

    Article  CAS  Google Scholar 

  19. Nabavi S.M., Ebrahimzadeh M.A., Nabavi S.F., Jafari M., Free radical scavenging activity and antioxidant capacity of Eryngium caucasicum Trautv and Froriepia subpinnata, Pharmacologyonline, 2008, 3, 19–25

    Google Scholar 

  20. Sreejayan N., Rao M.N.A., Nitric oxide scavenging by curcuminoids, J. Pharm. Pharmacol., 1997, 49, 105–107

    CAS  PubMed  Google Scholar 

  21. Ebrahimzadeh M.A., Nabavi S.F., Nabavi S.M., Essential oil composition and antioxidant activity of Pterocarya fraxinifolia, Pak. J. Biol. Sci., 2009, 12, 957–963

    Article  CAS  PubMed  Google Scholar 

  22. Dinis T.C.P., Madeira V.M.C., Almeida L.M., Action of phenolic derivates (acetoaminophen, salycilate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers, Arch. Biochem. Biophys., 1994, 315, 161–169

    Article  CAS  PubMed  Google Scholar 

  23. Ebrahimzadeh M.A., Pourmorad F., Bekhradnia A.R., Iron chelating activity screening, phenol and flavonoid content of some medicinal plants from Iran, Afr. J. Biotech., 2008, 7, 3188–3192

    CAS  Google Scholar 

  24. Ebrahimzadeh M.A., Nabavi S.M., Nabavi S.F., Correlation between the in vitro iron chelating activity and poly phenol and flavonoid contents of some medicinal plants, Pak. J. Biol. Sci., 2009, 12, 934–938

    Article  CAS  PubMed  Google Scholar 

  25. Kuo J.M., Yeh D.B., Pan B.S., Rapid photometric assay evaluating antioxidative activity in edible plant material, J. Agric. Food Chem., 1999, 47, 3206–3209

    Article  CAS  PubMed  Google Scholar 

  26. Song-Hwan B., Hyung-Joo S., Antioxidant activities of five different mulberry cultivars in Korea, Lebenson. Wiss. Technol. Food Sci. Technol., 2007, 40, 955–962

    Google Scholar 

  27. Dehpour A.A., Ebrahimzadeh M.A., Nabavi S.F., Nabavi S.M., Antioxidant activity of the methanol extract of Ferula assafoetida and its essential oil composition, Grasas Aceites, 2009, 60, 405–412

    Article  CAS  Google Scholar 

  28. Aldini G., Piccoli A., Beretta G., Morazzoni P., Riva A., Marinello C., et al., Antioxidant activity of polyphenols from solid olive residues of c.v. Coratina, Fitoterapia, 2006, 77, 121–128

    Article  CAS  PubMed  Google Scholar 

  29. Naim M., Gestener B., Bondi A., Birk Y., Antioxidant and antihemolytic activities of soyabean isoflavones, J. Agric. Food Chem., 1976, 24, 1174–1177

    Article  CAS  PubMed  Google Scholar 

  30. Yuan X., Wang J., Yao H., Chen F., Free radical-scavenging capacity and inhibitory activity on rat erythrocyte hemolysis of feruloyl oligosaccharides from wheat bran insoluble dietary fiber, Lebenson. Wiss. Technol. Food Sci. Technol., 2005, 38, 877–883

    CAS  Google Scholar 

  31. Narendhirakannan R.T., Subramanian S., Kandaswamy M., Mineral content of some medicinal plants used in the treatment of diabetes mellitus, Biol. Trace Elem. Res., 2005, 103, 109–115

    Article  CAS  PubMed  Google Scholar 

  32. Van Acker S.A.B.E., van Den Berg D.J., Tromp M.N.J.L., Griffioen D.H., Van Bennekom W.P., van der Vijgh W.J.F., et al., Structural aspects of antioxidant activity of flavanoids, Free Radic. Biol. Med., 1996, 20, 331–342

    Article  PubMed  Google Scholar 

  33. Ebrahimzadeh M.A., Bahramian F., Antioxidant activity of Crataegus pentagina subsp. elbursis fruits extracts used in traditional medicine in Iran, Pak. J. Biol. Sci., 2009, 12, 413–419

    Article  CAS  PubMed  Google Scholar 

  34. Nabavi S.M., Ebrahimzadeh M.A., Nabavi S.F., Bahramian F., In Vitro antioxidant activity of Phytolacca Americana Berries, Pharmacologyonline, 2009, 1, 81–88

    Google Scholar 

  35. Moncada A., Palmer R.M.J., Higgs E.A., Nitric oxide: physiology, pathophysiology and pharmacology, Pharmacol. Rev., 1991, 43, 109–142

    CAS  PubMed  Google Scholar 

  36. Ebrahimzadeh M.A., Nabavi S.M., Nabavi S.F., Eslami B., Antioxidant activity of aqueous extract of Pyrus boissieriana fruit, Pharmacologyonline, 2009, 1, 1318–1323

    Google Scholar 

  37. Halliwell B., Gutteridge J.M.C., Role of free radicals and catalytic metal ions in human disease: an overview, Meth. Enzymol., 1990, 186, 81–85

    Google Scholar 

  38. Hebbel RP, Leung A, Mohandas N. 1990. Oxidation-induced changes in microheological properties of the red cell membrane, Blood 76, 1015–1022.

    CAS  PubMed  Google Scholar 

  39. Grazul M., Budzisz E., Biological activity of metal ions complexes of chromones, coumarins and flavones, Coord. Chem. Rev., 2009, 253, 2588–2598

    Article  CAS  Google Scholar 

  40. Ebrahimzadeh M.A., Nabavi S.F., Nabavi S.M., Eslami B., Antihypoxic and antioxidant activity of Hibiscus esculentus seeds, Grasas Aceites, 2010, 61, 30–36

    Article  CAS  Google Scholar 

  41. Ebrahimzadeh M.A., Nabavi S.F., Nabavi S.M., Antioxidant activities of methanol extract of sambucus ebulus L. flower, Pak. J. Biol. Sci., 2009, 12, 447–450

    Article  CAS  PubMed  Google Scholar 

  42. Ebrahimzadeh M.A., Ehsanifar S., Eslami B., Sambucus ebulus elburensis fruits: A good source for antioxidants, Pharmacognosy Magazine, 2009, 4, 213–218

    Google Scholar 

  43. Djeridane A., Yousfi M., Nadjemi B., Vidal N., Lesgards J.F., Stocker P., Screening of some Algerian medicinal plants for the phenolic compounds and their antioxidant activity, Eur. Food Res. Tech., 2006, 224, 801–809

    Article  Google Scholar 

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Authors and Affiliations

  1. Pharmaceutical Sciences Research Center, School of Pharmacy, Mazandaran University of Medical Sciences, 48189, Sari, Iran

    Mohammad Ali Ebrahimzadeh, Seyed Fazel Nabavi & Seyed Mohammad Nabavi

  2. Student Research Development Committee, Mazandaran University of Medical Sciences, 48189, Sari, Iran

    Seyed Fazel Nabavi

  3. Department of Biology, University of Mazandaran, 48181-59376, Babolsar, Iran

    Seyed Mohammad Nabavi

  4. Department of Biology, Islamic Azad University, Ghaemshahr Branch, 32549, Ghaemshahr, Iran

    Bahman Eslami

Authors
  1. Mohammad Ali Ebrahimzadeh
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  2. Seyed Fazel Nabavi
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  3. Seyed Mohammad Nabavi
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  4. Bahman Eslami
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Correspondence to Mohammad Ali Ebrahimzadeh.

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Ebrahimzadeh, M.A., Nabavi, S.F., Nabavi, S.M. et al. Antihemolytic and antioxidant activities of Allium paradoxum . cent.eur.j.biol. 5, 338–345 (2010). https://doi.org/10.2478/s11535-010-0013-5

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