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Collagen-bound LDL modifies endothelial cell adhesion to type V collagen: Implications for atherosclerosis

  • Research Article
  • Published:
Central European Journal of Biology

Abstract

Low density lipoprotein (LDL) is retained in the extracellular matrix of the arterial wall where it is considered to be atherogenic, but little is known about how cell adhesion to the matrix is affected by collagen-bound LDL. We tested the effect of native, oxidized and acetylated LDL reacted with adsorbed monomeric type I, III and V collagen on endothelial cell adhesion to collagen using a colorimetric adhesion assay. We found that none of the LDL species affected adhesion to type I and III collagen, but that collagen-bound native and acetylated LDL enhanced attachment to type V collagen, whereas bound oxidized LDL inhibited adhesion to this collagen. We therefore suggest that oxidized LDL associated with type V collagen in the arterial wall would favor de-endothelialization and contribute to atherogenesis and thrombosis.

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Authors and Affiliations

  1. Institute of Nutrition, Friedrich Schiller University Jena, 07743, Jena, Germany

    Stefan Lorkowski

  2. Leibniz Institute of Arteriosclerosis Research Münster, 48149, Münster, Germany

    Jürgen Rauterberg, Bärbel Harrach-Ruprecht & David Troyer

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  1. Stefan Lorkowski
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  2. Jürgen Rauterberg
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  3. Bärbel Harrach-Ruprecht
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  4. David Troyer
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Correspondence to Stefan Lorkowski.

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Lorkowski, S., Rauterberg, J., Harrach-Ruprecht, B. et al. Collagen-bound LDL modifies endothelial cell adhesion to type V collagen: Implications for atherosclerosis. cent.eur.j.biol. 4, 536–542 (2009). https://doi.org/10.2478/s11535-009-0047-8

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  • DOI: https://doi.org/10.2478/s11535-009-0047-8

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