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Central European Journal of Biology

, Volume 4, Issue 2, pp 196–203 | Cite as

Novel bacteriocins produced by Geobacillus stearothermophilus

  • Karina Pokusaeva
  • Nomeda Kuisiene
  • Dziuginta Jasinskyte
  • Kazimiera Rutiene
  • Jordana Saleikiene
  • Donaldas Chitavichius
Research Article

Abstract

Four novel heat-stable bacteriocin-like substances were found to be produced by Geobacillus stearothermophilus strains isolated from oil-wells in Lithuania. Geobacillus stearothermophilus 32A, 17, 30 and 31 strains were identified as producers of bacteriocins with bactericidal activity against closely related Geobacillus species and several pathogenic strains: Bacillus cereus DSM 12001 and Staphylococcus haemolyticus P903. The secretion of the analysed bacteriocins started during early logarithmic growth and dropped sharply after the culture entered the stationary phase of growth. The antimicrobial activity of the bacteriocins against sensitive indicator cells disappeared after treatment with proteolytic enzymes, indicating their proteinaceous nature. Bacteriocins were stable throughout the pH range between 4 and 10, and no loss in activity was noted following temperature exposures up to 100°C. Direct detection of antibacterial activity on SDS-PAGE suggests that the inhibitory peptides have a molecular weight of 6–7.5 kDa. Such bacteriocins with broad activity spectra, including antipathogenic action, are attractive to the biotechnology industry as they could be used as antimicrobial agents in medicine, agriculture and food products.

Keywords

Bacteriocin Geobacillus stearothermophilus Antimicrobial peptide Partial purification Thermocin 

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Copyright information

© © Versita Warsaw and Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  • Karina Pokusaeva
    • 1
  • Nomeda Kuisiene
    • 1
  • Dziuginta Jasinskyte
    • 1
  • Kazimiera Rutiene
    • 2
  • Jordana Saleikiene
    • 2
  • Donaldas Chitavichius
    • 1
  1. 1.Department of Plant Physiology and Microbiology, Faculty of Natural SciencesVilnius UniversityVilniusLithuania
  2. 2.National Public Health Laboratory CentreVilniusLithuania

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