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DNA-based biosensors with external Nafion and chitosan membranes for the evaluation of the antioxidant activity of beer, coffee, and tea

  • Research Article
  • Published:
Central European Journal of Chemistry

Abstract

Novel electrochemical DNA-based biosensors with outer-sphere Nafion and chitosan protective membranes were prepared for the evaluation of antioxidant properties of beverages (beer, coffee, and black tea) against prooxidant hydroxyl radicals. A carbon working electrode of a screen-printed three-electrode assembly was modified using a layer-by-layer deposition technique with low molecular weight double-stranded DNA and a Nafion or chitosan film. The membrane-covered DNA biosensors were initially tested with respect to their voltammetric and impedimetric response after the incubation of the beverage and the medium exchange for the solution of the redox indicator [Fe(CN)6]3−/4−. While the Nafion-protected biosensor proved to be suitable for beer and black tea extracts, the chitosan-protected biosensor was successfully used in a coffee extract. Afterwards, the applicability was successfully verified for these biosensors for the detection of a deep degradation of the surface-attached DNA at the incubation in the cleavage agent (hydroxyl radicals generated via Fenton reaction) and for the evaluation of antioxidant properties of coffee and black tea extracts against prooxidant hydroxyl radicals. The investigation of the novel biosensors with a protective membrane represents a significant contribution to the field of electrochemical DNA biosensors utilization.

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Authors and Affiliations

  1. Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, 812 37, Bratislava, Slovakia

    Lenka Hlavatá, Katarína Beníková, Monika Borbélyová & Ján Labuda

  2. UNESCO Laboratory of Environmental Electrochemistry, Department of Analytical Chemistry, University Research Centre UNCE “Supramolecular Chemistry”, Charles University in Prague, Faculty of Science, 128 43, Prague 2, Czech Republic

    Vlastimil Vyskočil

Authors
  1. Lenka Hlavatá
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  2. Vlastimil Vyskočil
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  3. Katarína Beníková
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  4. Monika Borbélyová
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  5. Ján Labuda
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Corresponding authors

Correspondence to Lenka Hlavatá or Vlastimil Vyskočil.

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Hlavatá, L., Vyskočil, V., Beníková, K. et al. DNA-based biosensors with external Nafion and chitosan membranes for the evaluation of the antioxidant activity of beer, coffee, and tea. cent.eur.j.chem. 12, 604–611 (2014). https://doi.org/10.2478/s11532-014-0516-4

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  • DOI: https://doi.org/10.2478/s11532-014-0516-4

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