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Potentiometric cholesterol biosensing application of graphene electrode with stabilized polymeric lipid membrane

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Central European Journal of Chemistry

Abstract

A novel potentiometric cholesterol biosensor has been fabricated through the immobilization of the stabilized polymeric lipid membrane onto graphene electrode. The stabilized polymeric lipid membrane is composed of cholesterol oxidase enzyme and polymerization mixture; which holds paramount influence on the properties of the cholesterol biosensor. The presented biosensor reveals an appreciable reproducibility, good selectivity and high sensing capability with a linear slope curve of ∼64 mV per decade. The strong biocompatibility among stabilized polymeric lipid membranes and human biofluids provides the possibility to use for real blood samples and other biological applications.

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Authors and Affiliations

  1. Laboratory of Inorganic & Analytical Chemistry, School of Chemical Engineering, Dept 1, Chemical Sciences, National Technical University of Athens, GR-157 80, Athens, Greece

    Georgia-Paraskevi Nikoleli & Nikolaos Tzamtzis

  2. Department of Science and Technology, Linköping University, Campus Norrköping, SE-60174, Norrköping, Sweden

    Zafar H. Ibupoto & Magnus Willander

  3. Laboratory of Environmental Chemistry, Department of Chemistry, University of Athens, Panepistimiopolis-Kouponia, GR-15771, Athens, Greece

    Dimitrios P. Nikolelis

  4. Institute of Advanced Materials, Physicochemical Processes, Nanotechnology and Microsystems — Division of Physical Chemistry, NCSR “Demokritos”, Aghia Paraskevi Attikis, GR-15341, Athens, Greece

    Vlassis Likodimos

  5. Laboratory of Inorganic Chemistry, Department of Chemistry, University of Athens, Panepistimiopolis-Kouponia, GR-15771, Athens, Greece

    Nikolas Psaroudakis

  6. Faculty of Mathematics, Physics and Informatics, Comenius University, 84248, Bratislava, Slovakia

    Tibor Hianik

Authors
  1. Georgia-Paraskevi Nikoleli
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  2. Zafar H. Ibupoto
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  3. Dimitrios P. Nikolelis
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  4. Vlassis Likodimos
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  5. Nikolas Psaroudakis
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  6. Nikolaos Tzamtzis
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  7. Magnus Willander
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  8. Tibor Hianik
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Correspondence to Dimitrios P. Nikolelis.

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Nikoleli, GP., Ibupoto, Z.H., Nikolelis, D.P. et al. Potentiometric cholesterol biosensing application of graphene electrode with stabilized polymeric lipid membrane. cent.eur.j.chem. 11, 1554–1561 (2013). https://doi.org/10.2478/s11532-013-0285-5

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  • DOI: https://doi.org/10.2478/s11532-013-0285-5

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